PMC:7796291 / 22189-26231 JSONTXT 2 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T180 0-4 Sentence denotes 3.4.
T181 5-56 Sentence denotes Bactericidal Performance of Disinfection Treatments
T182 57-189 Sentence denotes To investigate the disinfection efficacy, the number of survived colony on respirators after disinfection treatments was quantified.
T183 190-372 Sentence denotes The E. coli culture was loaded on the respirators applying physical pressure; the bacteria culture penetrated up to the stiffener, and did not penetrate beyond this layer (Figure 5).
T184 373-469 Sentence denotes Without the physical pressure, the loaded culture drop rolled around on the surface of coverweb.
T185 470-594 Sentence denotes The number of loaded E. coli by each layer was correlated with OD470 measurement and expressed as CFU/cm2 substrate surface.
T186 595-876 Sentence denotes Bacteria inactivation or removal efficiency was calculated by: (B0−Bn)B×100 (%), where B0 is the number of live bacteria in the untreated sample area, and Bn is the number of live bacteria in the treated sample area (n = 1~8, each number indicating the eight disinfection methods).
T187 877-929 Sentence denotes Bactericidal or bacteria removal efficiency of Resp.
T188 930-967 Sentence denotes A is shown in Figure 6 and Figure S7.
T189 968-1124 Sentence denotes In the 2.5 cm × 2.5 cm designated area of each web, non-treated samples showed ~1.46 × 106 and ~2.53 × 106 CFUs on the coverweb and stiffener, respectively.
T190 1125-1328 Sentence denotes Microwaving, oven-dry, and UV treatment showed similar bactericidal effect up to about 82%; in fact, this is inconsistent with previous studies [18,20], where 99.99% inactivation efficiency was achieved.
T191 1329-1480 Sentence denotes This discrepancy can be attributed to the different treatment conditions, such as irradiation intensity, treatment time, and amount of bacteria loaded.
T192 1481-1706 Sentence denotes In a previous study, 1.0 × 106 CFU of bacteria was loaded on the respirator with a nebulizer; and the UV irradiation (wavelength; 254 nm, 5 min) and microwaving (400 W, 10 min) resulted in complete inactivation of cells [18].
T193 1707-1887 Sentence denotes The case of an oven-dry treatment (100 °C, 15 min) also achieved 99.99% inactivation efficiency, when 5.0 × 104 CFU of bacteria was dropped onto the surface of the respirator [20].
T194 1888-2018 Sentence denotes Compared to previous studies, this study built a harsher experimental condition with a higher loading of bacteria (5.0 × 106 CFU).
T195 2019-2211 Sentence denotes As a result, the intensity of microwaving (750 W, 2 min), UV irradiation (wavelength; 253.7 nm, 2 h), and oven-dry (90 °C, 1 h) of this study were insufficient for complete sterilization [51].
T196 2212-2406 Sentence denotes Chemical solvents (ClO−, EtOH, and IPA) and laundering treatments showed no color change with cell staining, indicating 100% bactericidal or bacteria removal efficiency (Figure 6 and Figure S7).
T197 2407-2515 Sentence denotes The morphological state of loaded bacteria after disinfection treatments was analyzed by FE-SEM (Figure 6C).
T198 2516-2725 Sentence denotes With microwaving, oven-dry, and UV irradiation, disruption of E. coli membrane integrity was observed; on the contrary, IPA immersion and water-laundering resulted in both cell deformation and cell detachment.
T199 2726-2854 Sentence denotes Remaining bacteria on water-laundered media was observed in a tangled structure of cells with contaminants on the fiber surface.
T200 2855-3022 Sentence denotes Generally, microwaving and oven-drying rely solely on the heating effect to inactivate microorganisms [52], as heating triggers the denaturation of cell proteins [53].
T201 3023-3191 Sentence denotes Additionally, the UV irradiation breaks the strong bond of cell membranes, such as O-H, P-O, and N-H bonds of E. coli, or induces oxidation, leading to cell death [54].
T202 3192-3458 Sentence denotes The solvents and detergent laundering treatments have compound effect of bactericidal and bacteria removal activity, by accumulating in the lipid of membrane and degrading the membrane integrity while inducing suspension of bacterial droplet into the liquid [55,56].
T203 3459-3575 Sentence denotes Meanwhile, laundering effectively removed bacteria by detaching them from fibers, rather than sterilizing the cells.
T204 3576-3771 Sentence denotes The results of bactericidal performance test imply the chemical solvents and hypochlorite solution are effective disinfection agents, as they concurrently inactivate the cell and detach bacteria.
T205 3772-3912 Sentence denotes Even if it is not complete sterilization, UV irradiation, and oven-dry methods seem fairly applicable for inactivating cells to some extent.
T206 3913-4042 Sentence denotes Laundering treatment removed a considerable amount of loaded bacteria; additionally, even the remaining bacteria was inactivated.