| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T5 |
154-317 |
Sentence |
denotes |
Manufacturing of this N-terminally acetylated 28-residue peptide is demanding, and its short plasma half-life limits in vivo efficacy and requires frequent dosing. |
| T6 |
318-481 |
Sentence |
denotes |
Here, we combined the PASylation technology with enzymatic in situ N-acetylation by RimJ to produce a long-acting version of Tα1 in Escherichia coli at high yield. |
| T7 |
482-597 |
Sentence |
denotes |
ESI-MS analysis of the purified fusion protein indicated the expected composition without any signs of proteolysis. |
