LitCovid-sentences  

PMC:7594251 / 104267-105469 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T694 0-4 Sentence denotes 4.2.
T695 5-32 Sentence denotes Target-Detected In-Cell NMR
T696 33-148 Sentence denotes In the target-detected in-cell NMR only the target of interest is isotopically labeled (i.e., 15N labeled protein).
T697 149-291 Sentence denotes For instance, target proteins can be isotopically labeled during cell growth in isotopically enriched (13C, 15N, or both 15N/13C) media [410].
T698 292-358 Sentence denotes The cell type and the labeling method may vary across experiments.
T699 359-546 Sentence denotes Different cell types, including bacteria [411], oocytes [412], yeast cells [413], mammalian cells [414], HeLa cells [415] and even insect cells [416] have been reported in the literature.
T700 547-681 Sentence denotes The fact that in-cell NMR applies to more than one cell type testifies of the versatility and potential application of this technique.
T701 682-800 Sentence denotes In terms of labeling, 15N is one of the most commonly used approaches [417] when the targets of interest are proteins.
T702 801-898 Sentence denotes Recently, 19F labeling has been reported as a useful probe for protein-ligand interactions [418].
T703 899-1000 Sentence denotes It was shown that 19F can reveal information about the dynamics of protein-ligand interactions [419].
T704 1001-1202 Sentence denotes Methyl groups [420] have also been used as probes for proteins and complexes in vivo [420], proving that labeling specific chemical groups instead of the entire biomolecule (i.e., protein) is feasible.