| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T45 |
0-83 |
Sentence |
denotes |
To detect DNA production in RT-LAMP assays, various approaches have been described. |
| T46 |
84-198 |
Sentence |
denotes |
One possibility is to use a pH indicator (e.g., phenol red) and run the reaction in a weakly buffered environment. |
| T47 |
199-370 |
Sentence |
denotes |
As the chain reaction proceeds, the pH is lowered, which results in a visible color change from red to yellow making it an appealing assay for point-of-care diagnosis (8). |
| T48 |
371-492 |
Sentence |
denotes |
Previously, RT-LAMP assays have been proposed for diagnostic detection of other RNA viruses, such as influenza virus (9). |
| T49 |
493-615 |
Sentence |
denotes |
Also, several studies have demonstrated the use of isothermal DNA amplification to detect small amounts of SARS-CoV-2 RNA. |
| T50 |
616-815 |
Sentence |
denotes |
The majority of these studies used in vitro transcribed (IVT) short fragments of the viral genomic RNA (10–12) and showed a detection limit of somewhere between 10 and 100 RNA molecules per reaction. |
| T51 |
816-1068 |
Sentence |
denotes |
For the detection of SARS-CoV-2 RNA, a few commercial rapid tests have been developed [reviewed in (13)] using isothermal DNA amplification reactions involving proprietary enzyme formulations that are not commercially available in a ready-to-go format. |
| T52 |
1069-1209 |
Sentence |
denotes |
Further, their exact sensitivity is still subject to discussion owing to a lack of studies using sufficiently large numbers of test samples. |
