LitCovid-sentences  

PMC:7574920 / 51237-52509 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T371 0-21 Sentence denotes Swab–to–RT-LAMP assay
T372 22-137 Sentence denotes For direct and hot swab–to–RT-LAMP assays, patient swab specimens were transferred first onto a 96-well seed plate.
T373 138-298 Sentence denotes For the direct assay, we then transferred 1 μl of the specimen directly to 19 μl of LAMP mix per well in a ready-made 96-well PCR plate (0030128672, Eppendorf).
T374 299-417 Sentence denotes The plate was sealed using a transparent adhesive foil (GK480-OS, Kisker Biotech) and kept on an ice-cold metal block.
T375 418-599 Sentence denotes For the hot assay, we sealed the seed plate with a pierceable lid (4ti-0566/96, Brooks Life Sciences) and heated it in a PCR cycler for 5 min at 95°C (with the lid heated to 105°C).
T376 600-665 Sentence denotes The seed plate was cooled down to 4°C on an ice-cold metal block.
T377 666-799 Sentence denotes Afterward, 1 μl of the heat-treated patient specimens was quickly added to a second ready-made plate with 19 μl of LAMP mix per well.
T378 800-885 Sentence denotes This plate was also sealed with transparent adhesive foil (GK480-OS, Kisker Biotech).
T379 886-959 Sentence denotes Both plates were then incubated at 65°C for the LAMP reaction to proceed.
T380 960-1121 Sentence denotes For both swab–to–RT-LAMP assays, the PCR plates were briefly spun down and then incubated in a PCR cycler at 65°C for 10 to 60 min (with the lid heated to 75°C).
T381 1122-1272 Sentence denotes To perform measurements at the indicated time points, the reactions were taken out of the PCR cycler and placed into an ice-cold metal block for 30 s.