| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T315 |
0-12 |
Sentence |
denotes |
Study design |
| T316 |
13-198 |
Sentence |
denotes |
The intent of this study was to develop a clinical method for detecting SARS-CoV-2 RNA in RNA samples isolated from pharyngeal swab specimens from individuals being tested for COVID-19. |
| T317 |
199-410 |
Sentence |
denotes |
We used pseudo-anonymized surplus RNA sample material that had been collected for clinical diagnosis of SARS-CoV-2 infection by RT-qPCR carried out by the diagnostic laboratory of Heidelberg University Hospital. |
| T318 |
411-617 |
Sentence |
denotes |
Such reuse of material is in accordance with German regulations, which allow development and improvement of diagnostic assays using patient samples collected specifically to perform the testing in question. |
| T319 |
618-792 |
Sentence |
denotes |
Pharyngeal swab specimens provided to us were either collected through the nose (nasopharyngeal) or the mouth (oropharyngeal), or sometimes one swab was used to collect both. |
| T320 |
793-1046 |
Sentence |
denotes |
Our study was designed to investigate the sensitivity and specificity of a colorimetric RT-LAMP assay and to evaluate its suitability as an alternative to RT-qPCR testing for detecting SARS-CoV-2 viral RNA in RNA isolated from pharyngeal swab specimens. |
| T321 |
1047-1122 |
Sentence |
denotes |
This study was conducted in Heidelberg, Germany in March and April of 2020. |
| T322 |
1123-1401 |
Sentence |
denotes |
The study was designed to first evaluate different existing primer sets for RT-LAMP reactions and to use them for (i) detection of SARS-CoV-2 RNA in RNA isolated from pharyngeal swabs and (ii) detection of SARS-CoV-2 RNA directly from swab specimens without prior RNA isolation. |
| T323 |
1402-1676 |
Sentence |
denotes |
All RNA samples used were pseudo-anonymized surplus material from the Heidelberg University Hospital diagnostic laboratory, and RT-qPCR results for these RNA samples were retrieved from the laboratory’s database only after the samples had been analyzed by the RT-LAMP assay. |
| T324 |
1677-1826 |
Sentence |
denotes |
The study design was to conduct RT-LAMP testing until sufficient samples (at least several hundreds) had been analyzed to obtain a conclusive result. |
| T325 |
1827-2006 |
Sentence |
denotes |
We also designed a deep sequencing-based method to validate the outcome of the RT-LAMP reactions using a Tn5 transposase–based fully scalable barcoding strategy (LAMP-sequencing). |
