| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T697 |
0-48 |
Sentence |
denotes |
Crystallization/Soak Protocols for SARS1 with 28 |
| T698 |
49-143 |
Sentence |
denotes |
Crystals of compound 28 bound to SARS1/CoV-1 DC2 protease were produced via cocrystallization. |
| T699 |
144-257 |
Sentence |
denotes |
SARS1/CoV-1 DC2 protease at 10.00 mg/mL was incubated with a 3-fold molar excess of compound 28 for 18 h at 4 °C. |
| T700 |
258-415 |
Sentence |
denotes |
The complex was then passed through a 0.45 μM cellulose acetate spin filter and set up for crystallization using an NT-8 crystallization robot (Formulatrix). |
| T701 |
416-684 |
Sentence |
denotes |
Using MRC-2 crystallization plates, wells containing 40 μL of 10% w/v PEG 8000, 0.2 M sodium chloride, 0.1 M sodium potassium phosphate pH 6.2, and 10 mM TCEP were dispensed, and then sitting drops consisting of 0.3 μL protein were set up against a 0.3 μL well buffer. |
| T702 |
685-807 |
Sentence |
denotes |
Crystallization plates were incubated at 21 °C, and rectangular crystals measuring 0.1 × 0.25 × 0.25 mm3 grew within 24 h. |
| T703 |
808-942 |
Sentence |
denotes |
Cocrystals were flash-frozen in liquid nitrogen after being passed through a cryo consisting of a well buffer containing 20% glycerol. |
