PMC:7543426 / 9923-15831 JSONTXT 11 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T109 0-13 Sentence denotes Lung Findings
T110 15-36 Sentence denotes Macroscopic Findings.
T111 37-171 Sentence denotes Lungs from all patients were increased in volume, firm, edematous, and congested with diffuse pleural thickening and pleural effusion.
T112 172-314 Sentence denotes Cut surface showed consolidation of lobes and red congested areas, with thickening of the interstitial septa and pulmonary edema (Figure 1A1).
T113 315-367 Sentence denotes Subsegmental pulmonary embolism was seen in 5 cases.
T114 368-377 Sentence denotes Figure 1.
T115 379-409 Sentence denotes Pathological findings in lung.
T116 410-489 Sentence denotes A1, Lungs showed an increase in volume and were firmer and heavier than normal.
T117 490-682 Sentence denotes A2 and A3, Light microscopic analysis shows parenchymal multifocal damage with intraalveolar inflammation, fibrin, and hyaline membranes consistent with a diagnosis of diffuse alveolar damage.
T118 683-768 Sentence denotes Both acute exudative inflammatory process and fibrous proliferative phase were found.
T119 769-912 Sentence denotes A3 and A4, Hyperplasia of type II pneumocyte, characterized by amphophilic cytoplasm, large nuclei, and prominent nucleoli, are shown (arrows).
T120 913-940 Sentence denotes B1, Alveolar duct fibrosis.
T121 941-975 Sentence denotes B2, Fibrin thrombi in capillaries.
T122 976-1043 Sentence denotes B3, Vascular injury including vessel vasculitis and vascular edema.
T123 1044-1065 Sentence denotes B4, Pleural fibrosis.
T124 1066-1108 Sentence denotes Abbreviation: H&E, haematoxylin and eosin.
T125 1109-1155 Sentence denotes Scale bars: A2, 14 µm; A3–B3, 7 µm; B4, 21 µm.
T126 1157-1178 Sentence denotes Microscopic Findings.
T127 1179-1241 Sentence denotes A range of common lung findings were seen on light microscopy.
T128 1242-1455 Sentence denotes Parenchymal multifocal damage with intraalveolar exudative and proliferative inflammation (Figure 1A2), with fibrin, hyaline membranes (Figure 1A3) consistent with a diagnosis of diffuse alveolar damage (Table 3).
T129 1456-1661 Sentence denotes Organizing pneumonia with fibrosis and type II pneumocyte hyperplasia, amphophilic cytoplasm, large nuclei, and prominent nucleoli, were indicative of cytopathic virus-induced changes (Figure 1A3 and 1A4).
T130 1662-1806 Sentence denotes Fibroblastic foci consisting of loose organizing connective tissue reflective of alveolar duct fibrosis were seen (fibrotic phase) (Figure 1B1).
T131 1807-1934 Sentence denotes Other findings included: pleural fibrosis (Figure 1B4), vascular injury with thrombi (Figure 1B2), and vasculitis (Figure 1B3).
T132 1935-2243 Sentence denotes A main feature was the presence of numerous inflammatory cells consisting of granulocytes (CD15+) (Supplementary Figure 1A1 and 1A2), macrophages (CD68+) (Supplementary Figure 1A3 and 1A4), and T lymphocytes (CD3+) infiltrating into alveolar septa and clustering around capillary vessels (Supplementary 1B1).
T133 2244-2356 Sentence denotes Immunohistochemistry showed the presence of both CD4+ and CD8+ T lymphocytes (Supplementary Figure 1B2 and 1B3).
T134 2357-2441 Sentence denotes Of note, lymphocytes were CD20− indicating an absence of infiltrating B lymphocytes.
T135 2442-2592 Sentence denotes Thrombi were present and immunohistochemistry showed the presence of platelet aggregates and megakaryocytes within thrombi (Supplementary Figure 1B4).
T136 2593-2601 Sentence denotes Table 3.
T137 2603-2626 Sentence denotes Histopathology Findings
T138 2627-2688 Sentence denotes Findings Group 1, No. (%) (n = 18) Group 2, No. (%) (n = 4)
T139 2689-2693 Sentence denotes Lung
T140 2694-2732 Sentence denotes  Hyaline membranes 8 (44.4) 2 (50.0)
T141 2733-2787 Sentence denotes  Hyperplasia of type II pneumocyte 11 (61.2) 4 (100)
T142 2788-2821 Sentence denotes  Alveolar plug 8 (44.4) 4 (100)
T143 2822-2866 Sentence denotes  Alveolar fibrin deposit 9 (50.0) 3 (75.0)
T144 2867-2899 Sentence denotes  Hemorrhage 10 (55.6) 2 (50.0)
T145 2900-2939 Sentence denotes  Inflammatory cells 13 (72.3) 4 (100)
T146 2940-3001 Sentence denotes  Diffuse alveolar damage, fibrotic phase 12 (66.7) 1 (25.0)
T147 3002-3036 Sentence denotes  Microthrombi 14 (77.8) 2 (50.0)
T148 3037-3067 Sentence denotes  Vasculitis 8 (44.4) 4 (100)
T149 3068-3115 Sentence denotes  Multinucleated giant cells 4 (22.3) 2 (50.0)
T150 3116-3123 Sentence denotes Cardiac
T151 3124-3156 Sentence denotes  Myocarditis 9 (50.0) 3 (75.0)
T152 3157-3188 Sentence denotes  Vasculitis 5 (27.8) 3 (75.0)
T153 3189-3234 Sentence denotes  Inflammatory infiltrate 13 (72.3) 3 (75.0)
T154 3235-3270 Sentence denotes  Focal necrosis 6 (33.4) 2 (50.0)
T155 3271-3303 Sentence denotes  Pericarditis 9 (50.0) 4 (100)
T156 3304-3342 Sentence denotes  Vascular fibrosis 4 (22.3) 2 (50.0)
T157 3343-3350 Sentence denotes Hepatic
T158 3351-3395 Sentence denotes  Inflammatory infiltrate 8 (44.4) 3 (75.0)
T159 3396-3427 Sentence denotes  Congestion 8 (44.4) 2 (50.0)
T160 3428-3458 Sentence denotes  Steatosis 9 (50.0) 3 (75.0)
T161 3459-3464 Sentence denotes Renal
T162 3465-3509 Sentence denotes  Inflammatory infiltrate 9 (50.0) 3 (75.0)
T163 3510-3549 Sentence denotes  Glomerulosclerosis 9 (50.0) 3 (75.0)
T164 3550-3591 Sentence denotes  Interstitial fibrosis 9 (50.0) 4 (100)
T165 3592-3599 Sentence denotes Splenic
T166 3600-3639 Sentence denotes  Congested red pulp 15 (83.4) 4 (100)
T167 3640-3680 Sentence denotes  Lymphoid hypoplasia 11 (61.2) 4 (100)
T168 3681-3692 Sentence denotes Bone marrow
T169 3693-3733 Sentence denotes  Megakaryocytes hyperplasia 0 2 (50.0)
T170 3734-3765 Sentence denotes  Adipocytes 7 (38.9) 1 (25.0)
T171 3767-3797 Sentence denotes Immunohistochemistry Findings.
T172 3798-3982 Sentence denotes T-cell activation is modulated by dipeptidyl peptidase-4 (DPP4) [16] and thus the expression of CD26/DPP4 and CXCR3/CD183 was analyzed on lung tissues from group 2 patients (Figure 2).
T173 3983-4121 Sentence denotes Numerous CD26+ cells were found in the alveolar septa (Figure 2A1–2A3) with intense staining seen in the type II pneumocytes (Figure 2A3).
T174 4122-4219 Sentence denotes Positive cells entrapped with fibrin clots in the vascular lumen were also observed (Figure 2A4).
T175 4220-4411 Sentence denotes CXCR3 immunostaining showed lymphocytes localized in inflammatory perivascular aggregates (Figure 2B1, 2B2, and 2B4) which spread into alveolar septa and alveolar spaces (Figure 2B2 and 2B3).
T176 4412-4421 Sentence denotes Figure 2.
T177 4423-4474 Sentence denotes Immunohistological characterization of lung tissue.
T178 4475-4536 Sentence denotes A1–A4, CD26/dipeptidyl peptidase-4 expression in lung tissue.
T179 4537-4607 Sentence denotes Numerous strongly positive CD26+ cells are seen in the alveolar septa.
T180 4608-4673 Sentence denotes A3, Intense staining is seen in the type II pneumocytes (arrows).
T181 4674-4751 Sentence denotes A4, Positive cells are also seen entrapped with fibrin in the vascular lumen.
T182 4752-4929 Sentence denotes B1–B4, CXCR3/CD183 immunostaining shows intense staining of lymphocytes localized in the inflammatory perivascular aggregates and spread into alveolar septa and alveolar spaces.
T183 4930-5005 Sentence denotes Scale bars: A1 and B1, 100 µm; A2, A3, and B4, 7 µm; A4, B2, and B3, 14 µm.
T184 5007-5037 Sentence denotes Electron Microscopic Findings.
T185 5038-5192 Sentence denotes SARS-CoV-2 particles were detected within type II pneumocytes, which showed degenerating features characterized by fine and uniformly dispersed chromatin.
T186 5193-5303 Sentence denotes These pneumocytes displayed swollen mitochondrial profiles and dilated rough endoplasmic reticulum (Figure 3).
T187 5304-5417 Sentence denotes Numerous virus-containing compartments (VCC) of different sizes and shapes were detected (Figure 3A, 3B, and 3D).
T188 5418-5537 Sentence denotes Interestingly, spherules, very small vesicles containing single viral particles (Figure 3B and 3C), were also observed.
T189 5538-5547 Sentence denotes Figure 3.
T190 5549-5621 Sentence denotes SARS-CoV-2 detection in lung tissue by transmission electron microscopy.
T191 5622-5723 Sentence denotes A, SARS-CoV-2 particles are visible in virus-containing compartments in type II pneumocytes (arrows).
T192 5724-5803 Sentence denotes B–D, Numerous viral particles are enclosed in single-membrane vacuoles (arrow).
T193 5804-5875 Sentence denotes Other, very small vesicles contain single viral particles (arrowheads).
T194 5876-5908 Sentence denotes Scale bars: A, 1 µm; B–D, 200nm.