Id |
Subject |
Object |
Predicate |
Lexical cue |
T475 |
0-272 |
Sentence |
denotes |
Recently, 95 full-length genomic sequences of SARAS-CoV-2 strains available in the National Center for Biotechnology Information and GISAID databases were subjected to multiple-sequence alignment and phylogenetic analyses for studying variations in the viral genome (260). |
T476 |
273-421 |
Sentence |
denotes |
All the viral strains revealed high homology of 99.99% (99.91% to 100%) at the nucleotide level and 99.99% (99.79% to 100%) at the amino acid level. |
T477 |
422-549 |
Sentence |
denotes |
Overall variation was found to be low in ORF regions, with 13 variation sites recognized in 1a, 1b, S, 3a, M, 8, and N regions. |
T478 |
550-679 |
Sentence |
denotes |
Mutation rates of 30.53% (29/95) and 29.47% (28/95) were observed at nt 28144 (ORF8) and nt 8782 (ORF1a) positions, respectively. |
T479 |
680-810 |
Sentence |
denotes |
Owing to such selective mutations, a few specific regions of SARS-CoV-2 should not be considered for designing primers and probes. |
T480 |
811-1025 |
Sentence |
denotes |
The SARS-CoV-2 reference sequence could pave the way to study molecular biology and pathobiology, along with developing diagnostics and appropriate prevention and control strategies for countering SARS-CoV-2 (260). |