| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T399 |
0-7 |
Sentence |
denotes |
Luminex |
| T400 |
8-104 |
Sentence |
denotes |
PBMCs from patients were thawed and rested overnight at 37°C in complete RPMI (cRPMI, table S7). |
| T401 |
105-219 |
Sentence |
denotes |
96-well flat bottom plates were coated with 1 μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. |
| T402 |
220-305 |
Sentence |
denotes |
The next day, cells were collected and plated at 1 × 105/well in 100 μl in duplicate. |
| T403 |
306-419 |
Sentence |
denotes |
2 μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). |
| T404 |
420-550 |
Sentence |
denotes |
PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and 85 μL/well of supernatant was collected. |
| T405 |
551-673 |
Sentence |
denotes |
Plasma from matched subjects was thawed on ice, spun (3000 rpm, 1 min) to remove debris, and 85 μl collected in duplicate. |
| T406 |
674-821 |
Sentence |
denotes |
Luminex assay was run according to manufacturer’s instructions, using a custom human cytokine 31-plex panel (EMD Millipore Corporation, SPRCUS707). |
| T407 |
822-1100 |
Sentence |
denotes |
The panel included: EGF, FGF-2, Eotaxin, sIL-2Ra, G-CSF, GM-CSF, IFN-α2, IFN-γ, IL-10, IL-12P40, IL-12P70, IL-13, IL-15, IL-17A, IL-1Ra, HGF, IL-1β, CXCL9/MIG, IL-2, IL-4, IL-5, IL-6, IL-7, CXCL8/IL-8, CXCL10/IP-10, CCL2/MCP-1, CCL3/MIP-1α, CCL4/MIP-1β, RANTES, TNF-α, and VEGF. |
| T408 |
1101-1194 |
Sentence |
denotes |
Assay plates were measured using a Luminex FlexMAP 3D instrument (Thermofisher, Cat#APX1342). |
| T409 |
1195-1288 |
Sentence |
denotes |
Data acquisition and analysis were done using xPONENT software www.luminexcorp.com/xponent/). |
| T410 |
1289-1347 |
Sentence |
denotes |
Data quality was examined based on the following criteria: |
| T411 |
1348-1462 |
Sentence |
denotes |
The standard curve for each analyte has a 5P R2 value > 0.95 with or without minor fitting using xPONENT software. |
| T412 |
1463-1654 |
Sentence |
denotes |
To pass assay technical quality control, the results for two controls in the kit needed to be within the 95% of CI (confidence interval) provided by the vendor for >25 of the tested analytes. |
| T413 |
1655-1730 |
Sentence |
denotes |
No further tests were done on samples with results out of range low (<OOR). |
| T414 |
1731-1905 |
Sentence |
denotes |
Samples with results that were out of range high (>OOR) or greater than the standard curve maximum value (SC max) were not tested at higher dilutions without further request. |
