| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T92 |
0-38 |
Sentence |
denotes |
Virus infection and immunofluorescence |
| T93 |
39-157 |
Sentence |
denotes |
All works with live virus were performed in the biosafety level (BSL)3 facility at the Public Health Agency of Sweden. |
| T94 |
158-359 |
Sentence |
denotes |
Vero-E6 cells were infected with SARS-CoV-2 (SARS-CoV-2-Iso/01/human/2020/SWE; GenBank accession number: MT093571) at a multiplicity of infection (MOI) of one in DMEM 2% FBS (Thermo Fisher Scientific). |
| T95 |
360-478 |
Sentence |
denotes |
At 24 hour post-infection, cells were fixed with chilled methanol/acetone and the cells were kept at −20 °C overnight. |
| T96 |
479-605 |
Sentence |
denotes |
Cells were then stained using mAb 1A9 at 5 µg/mL at 37 °C for 30 min in IF buffer (BSA 0.2%, Triton ×100 0.1% in PBS, pH 7.4). |
| T97 |
606-819 |
Sentence |
denotes |
The cells were washed three times with PBS and incubated, subsequently with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Thermo Fisher Scientific) in IF buffer containing DAPI for an additional 30 min. |
| T98 |
820-930 |
Sentence |
denotes |
Cells were washed three times with PBS before visualisation and image acquisition with fluorescent microscopy. |
