| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T870 |
0-26 |
Sentence |
denotes |
(Institut Pasteur, Paris). |
| T871 |
27-143 |
Sentence |
denotes |
Detection of viral genomes was performed by RT-qPCR, directly from the inactivated supernatant (Lista et al., 2020). |
| T872 |
144-200 |
Sentence |
denotes |
SARS-CoV-2 specific primers targeting the N gene region: |
| T873 |
201-451 |
Sentence |
denotes |
5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) (Chu et al., 2020) were used with the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermocycler, with the following cycling conditions: |
| T874 |
452-554 |
Sentence |
denotes |
55°C for 10 minutes, 95°C for 1 minute, and 40 cycles of 95°C for 10 s, followed by 60°C for 1 minute. |
| T875 |
555-730 |
Sentence |
denotes |
The number of viral genomes is expressed as PFU equivalents/mL, and was calculated by performing a standard curve with RNA derived from a viral stock with a known viral titer. |
