LitCovid-sentences  

PMC:7252096 / 93302-94913 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T667 0-39 Sentence denotes Nasal Washes during Influenza Infection
T668 40-554 Sentence denotes Nasal washes were obtained from adult healthy controls and from adults with diagnosis of acute influenza A or B by rapid antigen test (Flu A or B antigen, direct fluorescence antigen test) and/or by respiratory virus panel (PCR testing for influenza A, influenza A H1, influenza A H3, influenza B, adenovirus, metapneumovirus, respiratory syncytial virus A, respiratory syncytial virus B, rhino/enterovirus, parainfluenza 1, parainfluenza 2, parainfluenza 3), who show symptoms up to seven days (Cao et al., 2020).
T669 555-669 Sentence denotes Samples were obtained by irrigation of each naris with up to 10 mL of saline, and collected in a single container.
T670 670-744 Sentence denotes The sample was then transported to the research laboratory for processing.
T671 745-885 Sentence denotes Upon receipt, the sample was immediately stored on ice and 10 mL cell growth media (DMEM or RPMI1640 with 10% fetal bovine serum) was added.
T672 886-985 Sentence denotes The material was strained using a 40 μm nylon cell strainer (Corning) into a 50 mL centrifuge tube.
T673 986-1036 Sentence denotes Cells were pelleted at 1300 rpm for 10 min at 4°C.
T674 1037-1226 Sentence denotes All but 1 mL of supernatant was discarded, the pellet resuspended in the remaining 1 mL of supernatant, and material was transferred to an Eppendorf tube and pelleted at 2000 rpm for 5 min.
T675 1227-1494 Sentence denotes If the pellet contained visible blood, 200 μL of RBC lysis solution (ACK buffer, Thermo Fisher) was added to resuspend the pellet and incubated at room temperature for 2 min, after which 1 mL of cell media was added, and the cells were pelleted at 2000 rpm for 5 min.
T676 1495-1611 Sentence denotes The final pellet was resuspended in up to 1 mL of media and quantified before performing scRNA-seq with Seq-Well v1.