Id |
Subject |
Object |
Predicate |
Lexical cue |
T49 |
0-384 |
Sentence |
denotes |
The complete dataset of proteins available in (NCBI) [9] coronavirus data hub (https://www.ncbi.nlm.nih.gov/labs/virus/vssi/#/virus?SeqType_s=Nucleotide&VirusLineage_ss=Wuhan%20seafood%20market%20pneumonia%20virus,%20taxid:2697049&utm_campaign=wuhan_nCoV&utm_source=insights&utm_medium=referral) was retrieved and subjected to screening phase to identify potential vaccine candidates. |
T50 |
385-502 |
Sentence |
denotes |
First, host non-similar proteins of the pathogen were filtered that show no homology to the human host (taxonomic id: |
T51 |
503-509 |
Sentence |
denotes |
9606). |
T52 |
510-619 |
Sentence |
denotes |
Proteins having sequence E score < 1.0 E−5, bit score > 100, and sequence identity ≤ 30 % were selected [10]. |
T53 |
620-835 |
Sentence |
denotes |
Again, a BLASTp search against the Mouse (Mus musculus, taxonomy id: 10090) was performed using host non-similar proteins keeping the input parameters E score cut-off 0.005, bit score > 100, and identity < 30% [11]. |
T54 |
836-984 |
Sentence |
denotes |
The screened mouse non-similar proteins were then subjected to TMHMM 2.0 [12] and HMMTOP 2.0 [13] for observing the number of transmembrane helices. |
T55 |
985-1180 |
Sentence |
denotes |
Proteins having less than two transmembrane helices were subjected to SPAAN [14] for predicting the adhesive proteins as they have the potential to facilitate attachment to the host tissues [15]. |
T56 |
1181-1386 |
Sentence |
denotes |
Selected vaccine candidates were then used in BLASTp tool to align the selected adhesive protein candidates with the probiotic bacteria proteome including three Lactobacillus species: L. rhamnosus (tax id: |
T57 |
1387-1416 |
Sentence |
denotes |
47715), L. johnsonii (tax id: |
T58 |
1417-1457 |
Sentence |
denotes |
33959), and Lactobacillus casei (tax id: |
T59 |
1458-1527 |
Sentence |
denotes |
1582) to avoid accidental inhibition of the useful gut bacteria [11]. |