| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T115 |
0-44 |
Sentence |
denotes |
4.1.3 Serum from convalescent SARS patients |
| T116 |
46-167 |
Sentence |
denotes |
4.1.3.1 Hypothesis: SARS-CoV and SARS-CoV2 are ideally similar in the structure and the cell entry receptor and protease |
| T117 |
168-530 |
Sentence |
denotes |
SARS-CoV and SARS-CoV2 share absolutely the same cleavage junctions, almost the same sequence (96%) of their main protease, a high degree (76%) of similarity in the amino acid sequence of their S protein, a similar S2′ cleavage site, a similar spectrum of cells they can enter, and the similarity of the most residues essential for binding ACE2 [16], [17], [18]. |
| T118 |
531-616 |
Sentence |
denotes |
Also, both of them utilize the same domain of S1B to interact with the ACE2 receptor. |
| T119 |
617-679 |
Sentence |
denotes |
However, they differ in proteolytic processing to some degree. |
| T120 |
680-874 |
Sentence |
denotes |
Study [16] of the human embryonic kidney (HEK) cell line, 293 T, has shown that a signal for the S2 subunit is present in cells inoculated with SARS-2-S, but not in cells inoculated with SARS-S. |
| T121 |
875-1036 |
Sentence |
denotes |
Two main proteases for both SARS-S and SARS-2-S are endosomal cysteine proteases cathepsin B and L (CatB/L) and the transmembrane protease, serine 2TMPRSS2 [16]. |
| T122 |
1037-1216 |
Sentence |
denotes |
In 293 T cells lacking 2TMPRSS2, blocking CatB/L activity through increasing the endosomal pH by ammonium chloride could significantly limit the entry of both SARS-S and SARS-2-S. |
| T123 |
1217-1303 |
Sentence |
denotes |
In TMPRSS2 + Caco-2 cells, the effect of ammonium chloride existed to a lesser extent. |
| T124 |
1304-1473 |
Sentence |
denotes |
A combination of camostat mesylate, a blocker of TMPRSS2, and E-64d, an inhibitor of CatB/L, yielded the complete inhibition of SARS-2-S entry in TMPRSS2 + Caco-2 cells. |
| T125 |
1474-1723 |
Sentence |
denotes |
In both the human lung cancer cell line Calu-3 and the primary human lung cells, there was a reduction of the entry of both SARS-S and SARS-2-S by camostat mesylate, indicating that SARS-S and SARS-2-S partially require TMPRSS2 for a lung infection. |
| T126 |
1725-1743 |
Sentence |
denotes |
4.1.3.2 Rational: |
| T127 |
1744-1825 |
Sentence |
denotes |
Serum from convalescent SARS patients is able to neutralize SARS-CoV2 efficiently |
| T128 |
1826-2004 |
Sentence |
denotes |
Antiserum that contains antibodies against human ACE2 could hinder the entry of both SARS-S and SARS-2-S pseudotypes while not affected the entry of VSV-G and MERS-S pseudotypes. |
| T129 |
2005-2227 |
Sentence |
denotes |
It supports the notion that SARS-S and SARS-2-S utilize the same primary entry receptor, i.e., ACE2, which is different from the primary receptors VSV-G and MERS-S engage for cell entry that is LDLR and DPP4, respectively. |
| T130 |
2228-2340 |
Sentence |
denotes |
Sera from three convalescent SARS patients reduced the SARS-S entry and, to e lesser degree, the SARS-2-S entry. |
| T131 |
2341-2402 |
Sentence |
denotes |
The patient serum effect was in a dose-dependent manner [16]. |
