LitCovid-sentences  

PMC:7205724 / 14223-16625 JSONTXT 22 Projects

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Id Subject Object Predicate Lexical cue
T115 0-44 Sentence denotes 4.1.3 Serum from convalescent SARS patients
T116 46-167 Sentence denotes 4.1.3.1 Hypothesis: SARS-CoV and SARS-CoV2 are ideally similar in the structure and the cell entry receptor and protease
T117 168-530 Sentence denotes SARS-CoV and SARS-CoV2 share absolutely the same cleavage junctions, almost the same sequence (96%) of their main protease, a high degree (76%) of similarity in the amino acid sequence of their S protein, a similar S2′ cleavage site, a similar spectrum of cells they can enter, and the similarity of the most residues essential for binding ACE2 [16], [17], [18].
T118 531-616 Sentence denotes Also, both of them utilize the same domain of S1B to interact with the ACE2 receptor.
T119 617-679 Sentence denotes However, they differ in proteolytic processing to some degree.
T120 680-874 Sentence denotes Study [16] of the human embryonic kidney (HEK) cell line, 293 T, has shown that a signal for the S2 subunit is present in cells inoculated with SARS-2-S, but not in cells inoculated with SARS-S.
T121 875-1036 Sentence denotes Two main proteases for both SARS-S and SARS-2-S are endosomal cysteine proteases cathepsin B and L (CatB/L) and the transmembrane protease, serine 2TMPRSS2 [16].
T122 1037-1216 Sentence denotes In 293 T cells lacking 2TMPRSS2, blocking CatB/L activity through increasing the endosomal pH by ammonium chloride could significantly limit the entry of both SARS-S and SARS-2-S.
T123 1217-1303 Sentence denotes In TMPRSS2 + Caco-2 cells, the effect of ammonium chloride existed to a lesser extent.
T124 1304-1473 Sentence denotes A combination of camostat mesylate, a blocker of TMPRSS2, and E-64d, an inhibitor of CatB/L, yielded the complete inhibition of SARS-2-S entry in TMPRSS2 + Caco-2 cells.
T125 1474-1723 Sentence denotes In both the human lung cancer cell line Calu-3 and the primary human lung cells, there was a reduction of the entry of both SARS-S and SARS-2-S by camostat mesylate, indicating that SARS-S and SARS-2-S partially require TMPRSS2 for a lung infection.
T126 1725-1743 Sentence denotes 4.1.3.2 Rational:
T127 1744-1825 Sentence denotes Serum from convalescent SARS patients is able to neutralize SARS-CoV2 efficiently
T128 1826-2004 Sentence denotes Antiserum that contains antibodies against human ACE2 could hinder the entry of both SARS-S and SARS-2-S pseudotypes while not affected the entry of VSV-G and MERS-S pseudotypes.
T129 2005-2227 Sentence denotes It supports the notion that SARS-S and SARS-2-S utilize the same primary entry receptor, i.e., ACE2, which is different from the primary receptors VSV-G and MERS-S engage for cell entry that is LDLR and DPP4, respectively.
T130 2228-2340 Sentence denotes Sera from three convalescent SARS patients reduced the SARS-S entry and, to e lesser degree, the SARS-2-S entry.
T131 2341-2402 Sentence denotes The patient serum effect was in a dose-dependent manner [16].