LitCovid-sentences  

PMC:7152911 / 73517-75240 JSONTXT 7 Projects

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Id Subject Object Predicate Lexical cue
T605 0-72 Sentence denotes 2.4 Thermodynamics of pathogen-biorecognition element binding reactions
T606 73-334 Sentence denotes While the rate of biosensor response is typically governed by a mass transfer-limited heterogeneous reaction between the immobilized biorecognition element and target species, the net change in the biosensor response is dependent on the reaction thermodynamics.
T607 335-524 Sentence denotes The binding affinity between a biorecognition element and target species, such as an antibody and antigen, is often reported in terms of a dissociation constant (K D), which has units of M.
T608 525-735 Sentence denotes While the value of K D, solution = 1 nM provides a reasonable estimate for biosensor design considerations, such as understanding the mass transfer limitations associated with biosensor response (Squires et al.
T609 736-882 Sentence denotes 2008), the binding affinity of antibodies can vary by orders of magnitude depending on the pathogen of interest and the clonality of the antibody.
T610 883-1026 Sentence denotes One important consideration when immobilizing biorecognition elements is potential effects of immobilization on binding affinity to the target.
T611 1027-1099 Sentence denotes Traditionally, K D is obtained from a kinetic or thermodynamic analysis.
T612 1100-1238 Sentence denotes Kinetic analyses measure association and dissociation rate constants (k a and k d, respectively) and enable calculation of K D as k d/k a.
T613 1239-1533 Sentence denotes Thermodynamic analyses, such as calorimetric techniques, measure the binding enthalpy and entropy, which in turn provides the standard Gibbs free energy of the reaction (ΔG°), and thus, K A = K D −1 though the expression K A = exp(-ΔG°/RT), where R is the gas constant and T is the temperature.
T614 1534-1723 Sentence denotes A detailed discussion of the kinetics and thermodynamics of biorecognition element-target binding reactions for solution- and surface-based biosensors is provided in Supporting Information.