LitCovid-sentences  

PMC:7152911 / 67575-69255 JSONTXT 9 Projects

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Id Subject Object Predicate Lexical cue
T545 0-23 Sentence denotes 2.2.4 Oligonucleotides
T546 24-118 Sentence denotes Single-stranded DNA (ssDNA) is a useful biorecognition element for the detection of pathogens.
T547 119-287 Sentence denotes While ssDNA is commonly used as a biorecognition element for DNA-based assays, ssDNA aptamers are commonly used for pathogen detection using electrochemical biosensors.
T548 288-420 Sentence denotes Aptamers are single-stranded oligonucleotides capable of binding various molecules with high affinity and selectivity (Lakhin et al.
T549 421-444 Sentence denotes 2013; Reverdatto et al.
T550 445-451 Sentence denotes 2015).
T551 452-648 Sentence denotes Aptamers are isolated from a large random sequence pool through a selection process that utilizes systematic evolution of ligands by exponential enrichment, also known as SELEX (Stoltenburg et al.
T552 649-655 Sentence denotes 2007).
T553 656-784 Sentence denotes Suitable binding sequences can be isolated from a large random oligonucleotide sequence pool and subsequently amplified for use.
T554 785-866 Sentence denotes Thus, aptamers can exhibit high selectivity to target species (Stoltenburg et al.
T555 867-873 Sentence denotes 2007).
T556 874-981 Sentence denotes Aptamers can also be produced at a lower cost than alternative biorecognition elements, such as antibodies.
T557 982-1209 Sentence denotes Giamberardino et al. used SELEX to discover an aptamer for norovirus detection, which showed a million-fold higher binding affinity for the target than a random DNA strand that served as a negative control (Giamberardino et al.
T558 1210-1216 Sentence denotes 2013).
T559 1217-1368 Sentence denotes Iqbal et al. performed 10 rounds of SELEX to discover 14 aptamer clones with high affinities for C. parvum for detection in fruit samples (Iqbal et al.
T560 1369-1375 Sentence denotes 2015).
T561 1376-1673 Sentence denotes However, the use of aptamers as biorecognition elements has not yet replaced traditional biorecognition elements, such as antibodies, because of several challenges, such as aptamer stability, degradation, cross-reactivity, and reproducibility using alternative processing approaches (Lakhin et al.
T562 1674-1680 Sentence denotes 2013).