| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T357 |
0-256 |
Sentence |
denotes |
Future steps, which will result in a device for early-stage detection of, for example, zoonoses, include studies on the optimization of this device for sequence specific DNA amplifications (e.g., primer design and reaction optimization), i.e., HDA or LAMP. |
| T358 |
257-391 |
Sentence |
denotes |
HDA utilizes DNA helicase (an enzyme also used in vitro during DNA replication) to separate the dsDNA instead of thermal denaturation. |
| T359 |
392-630 |
Sentence |
denotes |
After separation, ssDNA binding proteins hybridize on the ssDNA strands for stabilization, ensuring that the next primer will have time to bind to the ssDNA stripe and a DNA polymerase will extend the primers with the complementary bases. |
| T360 |
631-800 |
Sentence |
denotes |
This method is a truly isothermal technique in which the separation of the dsDNA can be performed at the same temperature as the amplification reaction, i.e., 64 °C [9]. |
| T361 |
801-880 |
Sentence |
denotes |
LAMP is more similar to MDA in the way it also uses heat to denature the dsDNA. |
| T362 |
881-1065 |
Sentence |
denotes |
After denaturation, a set of four primers (six can be used as well to achieve better selectivity) and a DNA polymerase is used at isothermal conditions (65 °C) to amplify the DNA [11]. |
| T363 |
1066-1275 |
Sentence |
denotes |
When used in combination with reverse transcriptase, LAMP becomes a RNA amplification method, which could be used for RNA-containing viruses [11], like virus-based zoonoses diseases as the corona viruses [22]. |
| T364 |
1276-1394 |
Sentence |
denotes |
Despite not being a truly isothermal technique, LAMP offers the possibility to use turbidity as detection method [76]. |
| T365 |
1395-1554 |
Sentence |
denotes |
Such a detection method would simplify the required equipment even further as a decrease in transmitted light through the chip can be used as detection method. |
