LitCovid-sentences  

PMC:7143804 / 25711-27290 JSONTXT 9 Projects

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Id Subject Object Predicate Lexical cue
T197 0-152 Sentence denotes The chambers with the resistive heater on the backside, are intensively cleaned by rinsing with acetone, MilliQ DI water, ethanol, and isopropanol [45].
T198 153-231 Sentence denotes Each cleaning step was done 3 times and the chips are blow dried using N2 gas.
T199 232-455 Sentence denotes After drying, the chambers are closed using Microseal “B” PCR plate sealing foil from Bio-Rad (Bio-Rad Inc., Hercules, CA, USA), which is cut in the proper size and manually attached on top of the substrate (see Figure 4e).
T200 456-705 Sentence denotes The DNA, reactants and buffer solutions from the Illustra GenomiPhi V2 DNA amplification kit and an EvaGreen fluorescence dye are pipetted inside the chip using the inlet aperture, after which the inlet and outlet are closed using the same PCR foil.
T201 706-887 Sentence denotes An input potential is applied on the resistive heater using a Keithley 2602 SYSTEM SourceMeter (Cleveland, OH, USA) until they acquire the desired temperature for the amplification.
T202 888-1062 Sentence denotes The temperature is real-time monitored by inserting a 162 series RS Technics thermocouple K (RS Components B.V., Haarlem, The Netherlands) in the temperature monitor chamber.
T203 1063-1159 Sentence denotes The thermocouple is read out with a Tenma 72-7715 Thermometer (Premier Farnell Ltd., Leeds, UK).
T204 1160-1263 Sentence denotes The source and the read-out of the thermocouple are operated using a custom-programmed LabVIEW program.
T205 1264-1412 Sentence denotes The initial potential is based on the heater characterization measurements, but will be adjusted according to the feedback-loop of the thermocouple.
T206 1413-1579 Sentence denotes Detection of the amplification is done ex-situ by using quartz cuvets and an Horiba Scientific FluoroMax+ spectrofluorometer (Horiba Scientific, Piscataway, NJ, USA).