| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T139 |
0-234 |
Sentence |
denotes |
MDA is being done at temperatures of around 30 °C [50], which is lower than, for example, temperatures required for HDA (64 °C) [9] or LAMP (65 °C) [11] and the required PCR temperatures of Chung et al. (95 °C, 54 °C, and 72 °C) [28]. |
| T140 |
235-312 |
Sentence |
denotes |
However, most amplification methods require a DNA denaturation step at 95 °C. |
| T141 |
313-1002 |
Sentence |
denotes |
Equation (2) is used to make an estimation of the required heating powers for a COC–H2O–COC stack (in the real device, the upper plate is an adhesive PCR foil, but the thermal properties of this foil are unknown). (2) P=ΔTRth=ΔT∗Aheated∗κCOClCOC,1+κH2OlH2O+κCOClCOC,2+h Here, Rth is defined as the sum of all thermal resistances in series: (3) Rth=1h×Aheated+∑isubstancesliκi×Aheated Here, P is the required power, ΔT is the temperature difference, Rth is the thermal resistance, Aheated is the heated area, h is the convective heat transfer coefficient (being 10 W m−2 K−1 for convection to air [56]), κi is the thermal conductivity of substance i, and li is the thickness of substance i. |
| T142 |
1003-1044 |
Sentence |
denotes |
Values for κi can be found in Appendix B. |
| T143 |
1045-1125 |
Sentence |
denotes |
From Equation (3), the product Rth×A can be defined as the sum of 1/h and li/κi. |
| T144 |
1126-1348 |
Sentence |
denotes |
Based on this summation, one can conclude that the convective heat transfer to the air is the most present heat transfer mechanism within the system (begin almost a factor 100 higher than the heat lost in the COC and H2O). |
| T145 |
1349-1541 |
Sentence |
denotes |
This is also evident from solving Equation (2) for every individual temperature differences within the system and also including convective heat transfer directly from the heater into the air. |
| T146 |
1542-1802 |
Sentence |
denotes |
If a heated area of 7.7 mm by 10.1 mm is assumed, which covers both the reaction chamber and the temperature monitor chamber, and a system consisting of 1 mm COC–0.5 mm H2O–0.1 mm COC is assumed, than the heater temperatures and powers in Table 1 are required. |
| T147 |
1803-1916 |
Sentence |
denotes |
These are all in the workable range when a COC of a proper grade is chosen (e.g., TOPAS 6017 has a Tg of 170 °C). |
| T148 |
1917-2037 |
Sentence |
denotes |
The only side note here is that at higher temperatures, the temperature gradient through the system also becomes larger. |
| T149 |
2038-2115 |
Sentence |
denotes |
This can be eliminated by using double-sided heating, like Chung et al. [28]. |
