LitCovid-sentences  

PMC:7143804 / 10963-12766 JSONTXT 6 Projects

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Id Subject Object Predicate Lexical cue
T92 0-153 Sentence denotes The proof-of-principle amplification of choice is a MDA reaction, which is a non-specific isothermal method of amplification performed around 30 °C [50].
T93 154-244 Sentence denotes MDA is a method of whole genome amplification (WGA), as it amplifies all present DNA [52].
T94 245-315 Sentence denotes It is commonly used when the initial amount of DNA sample is very low.
T95 316-451 Sentence denotes After the WGA is performed, a sequence specific amplification can be done since the quality the amplified DNA by MDA is very high [53].
T96 452-588 Sentence denotes The amplification reaction is illustrated below in Figure 1 (the contour of the amplified sequence is highlighted in black for clarity).
T97 589-805 Sentence denotes Starting with a double stranded DNA (dsDNA) molecule, a denaturation step at 95 °C is required, giving the random hexamer-primers and the ϕ29 DNA polymerase access to the bases of single stranded DNA (ssDNA) strands.
T98 806-875 Sentence denotes The hexamers anneal themself to aleatory parts of the ssDNA sequence.
T99 876-944 Sentence denotes These hexamers work as initiation sites for the ϕ29 DNA polymerases.
T100 945-1059 Sentence denotes After denaturation at 95 °C, the mixture is cooled down to ice temperature and the rest of the reagents are added.
T101 1060-1260 Sentence denotes The mixture is heated up to ~30 °C so the polymerase starts to complete the complementary ssDNA sequence, creating again a dsDNA strand, eventually it encounters a hexamer from another annealing site.
T102 1261-1398 Sentence denotes Once this happens the polymerase will lift up that hexamer and starts to separate the amplified sequence formed from that annealing site.
T103 1399-1496 Sentence denotes As the polymerase displaces the formed strand ahead of it, it continues to complete the sequence.
T104 1497-1716 Sentence denotes The displaced strand becomes a new ssDNA strand and therefore, it gives new sites for more primers to attach and initiation sites for the polymerase, continuing the amplification, and thus creating a web of DNA strands.
T105 1717-1803 Sentence denotes Finally, the inactivation of the polymerase is done by heating up the system to 65 °C.