LitCovid-sentences  

PMC:7068163 / 1128-4777 JSONTXT 12 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T9 0-61 Sentence denotes Timely implementation of molecular diagnostics for SARS-CoV-2
T10 62-461 Sentence denotes In connection with the ongoing outbreak of a novel coronavirus in the province Hubei and surrounding areas in China, it was expected that Europe would also be confronted with the emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), as infections in travellers in several Asian countries outside of China were confirmed shortly after the announcement of the outbreak in Wuhan [1-4].
T11 462-628 Sentence denotes Therefore, it was necessary to rapidly implement adequately quick and sensitive diagnostic assays for outbreak management of SARS-CoV-2 in public health laboratories.
T12 629-800 Sentence denotes As soon as the World Health Organization (WHO) published the first protocols for real-time RT-PCR assays, the Bavarian Food and Health Authority started to implement them.
T13 801-939 Sentence denotes We ordered control material and oligonucleotides (see details below) in week 4 and ran our first SARS-CoV-2 assays on 27 January (week 5).
T14 940-1047 Sentence denotes On the same day, the first German case of coronavirus disease 2019 (COVID-19) was diagnosed in Bavaria [1].
T15 1048-1209 Sentence denotes In the following days, health authorities implemented comprehensive measures to prevent further transmission of SARS-CoV-2, including testing of contact persons.
T16 1210-1375 Sentence denotes We initially used the protocol based on the E gene and RdRp gene developed by the German Consiliary Laboratory for Coronaviruses hosted at the Charité in Berlin [5].
T17 1376-1573 Sentence denotes Real-time RT-PCR was initially performed with the QuantiTect Virus +Rox Vial kit (QIAGEN, Hilden, Germany) on the Bio-Rad CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Feldkirchen, Germany).
T18 1574-1696 Sentence denotes The kit was chosen for its frequent and successful use in our laboratory with other assays and its immediate availability.
T19 1697-1789 Sentence denotes Primers and probes were used as described [5] and provided by TIB Molbiol (Berlin, Germany).
T20 1790-2016 Sentence denotes Control material was ordered from the European Virus Archive (EVAg) and consisted of synthetic Wuhan coronavirus 2019 E gene control (reference number 026N-03866) and SARS-CoV Frankfurt 1 RNA (reference number 004N-02005) [6].
T21 2017-2155 Sentence denotes In addition, the control of LightMix Modular Wuhan CoV RdRP-gene (TibMolbiol, Berlin, Germany) was used for the SARS-CoV-2 specific assay.
T22 2156-2257 Sentence denotes Respiratory samples (nasopharyngeal swabs or sputum) were obtained from patients and contact persons.
T23 2258-2326 Sentence denotes Sputum samples were diluted in 2 mL phosphate buffered saline (PBS).
T24 2327-2446 Sentence denotes RNA was extracted using the QIAamp Bio Robot kit (QIAGEN) on a Hamilton Microlab Star (Hamilton, Bonaduz, Switzerland).
T25 2447-2765 Sentence denotes Each sample was tested by three PCRs: the screening assay for the E gene and two confirmatory assays targeting the RdRp gene, all performed as recommended in [5], with either both probes (RdRP_SARSr-P1 and RdRP_SARSr-P2), detecting SARS-CoV and SARS-CoV-2, or only with the SARS-CoV-2-specific probe RdRP_SARSr-P2 [5].
T26 2766-2822 Sentence denotes We used human RNAse P as control for RNA extraction [7].
T27 2823-2890 Sentence denotes The cumulative numbers of all tested samples are shown in Figure 1.
T28 2891-3039 Sentence denotes Two weeks after starting the SARS-CoV-2 diagnostics, we had analysed 669 respiratory samples at the Bavarian Health and Food Safety Authority (LGL).
T29 3040-3101 Sentence denotes Among them, seven showed positive results for SARS CoV-2 RNA.
T30 3102-3165 Sentence denotes By 11 February, 14 COVID-19 cases had been detected in Bavaria.
T31 3166-3275 Sentence denotes Figure 1 Cumulative numbers of suspected COVID-19 samples tested during week 5 and 6 2020, Bavaria (n = 675)
T32 3276-3365 Sentence denotes COVID: coronavirus disease; PHEIC: public health emergency of international concern; LGL:
T33 3366-3413 Sentence denotes Bavarian Health and Food Safety Authority; WHO:
T34 3414-3440 Sentence denotes World Health Organization.
T35 3441-3580 Sentence denotes The figure shows all samples tested at LGL, negative and positive, as well as six positive samples tested in other laboratories in Bavaria.
T36 3581-3649 Sentence denotes All data are cumulative, with a final total of positive tests of 13.