| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T129 |
0-4 |
Sentence |
denotes |
3.1. |
| T130 |
5-34 |
Sentence |
denotes |
Recovery of recombinant PEDVs |
| T131 |
35-272 |
Sentence |
denotes |
We have recently developed a reverse genetic platform of PEDV and have successfully generated a recombinant virus, iPEDVPT-P96, which is phenotypically comparable to its parental attenuated Taiwan PEDV-Pintung 52 strain, PEDVPT-P96 [19]. |
| T132 |
273-575 |
Sentence |
denotes |
To study the role of the S gene in the attenuation mechanism of PEDVPT-P96, we further generated the recombinant, highly virulent Taiwan PEDVPT 52 strain, iPEDVPT-P5, and two chimeric viruses (iPEDVPT-P5-96S and iPEDVPT-P96-5S) by replacing the complete sequence of the S gene reciprocally (Figure S1). |
| T133 |
576-692 |
Sentence |
denotes |
In general, the approach to rescue the new recombinant viruses were virtually the same as published previously [19]. |
| T134 |
693-928 |
Sentence |
denotes |
However, for the generation of iPEDVPT-P5 and iPEDVPT-P5-96S, we split the plasmid B to two fragments at nucleotide position of 9654 according to Hou et al. [23] in order to compensate for the instability caused by the toxic sequences. |
| T135 |
929-1091 |
Sentence |
denotes |
The in vitro and in vivo properties of iPEDVPT-P5 were confirmed similarly to those of the parental PEDVPT-P5 strain in a seven-day-old conventional piglet model. |
| T136 |
1092-1217 |
Sentence |
denotes |
At 24–48 h post-electroporation, typical PEDV cytopathic effects of giant syncytia were observed for all recombinant viruses. |
| T137 |
1218-1438 |
Sentence |
denotes |
Viral stocks were prepared by passaging viruses in Vero cells one additional time (P1), and the presence of PEDV was confirmed by detection of PEDV S protein by immunofluorescence assay (Figure 1A) and sequence analyses. |
