LitCovid-sentences  

PMC:7019868 / 15071-17051 JSONTXT 9 Projects

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Id Subject Object Predicate Lexical cue
T111 0-6 Sentence denotes 2.5.2.
T112 7-46 Sentence denotes Histopathology and Immunohistochemistry
T113 47-305 Sentence denotes At three days post-inoculation, three pigs from each virus-treated group and one pig from mock group were humanely euthanized by electrocution followed by exsanguination for histopathological and immunohistochemical assessments, as described previously [22].
T114 306-623 Sentence denotes Duodenum, jejunum, ileum, cecum, colon, rectum and mesenteric lymph nodes were collected, formalin-fixed, paraffin-embedded, sectioned at 4 μm, and stained routinely with hematoxylin and eosin (H&E) for morphometric analysis by assessing the ratio of villi height to crypt depth blindly by one veterinary pathologist.
T115 624-704 Sentence denotes Immunohistochemistry was performed to evaluate the distribution of PEDV antigen.
T116 705-952 Sentence denotes Briefly, formalin-fixed paraffin-embedded tissues were sectioned at 4 μm, deparaffined in xylene, rehydrated in serially diluted ethanol, and proceeded to epitope retrieval with the Trilogy antigen retrieval system (Cell Marque, Rocklin, CA, USA).
T117 953-1261 Sentence denotes After being washed three times with Tris-buffered saline plus 0.1% Tween 20 (TBST), tissue slides were treated with 3% hydrogen peroxidase (KYB, New Taipei City, Taiwan) and 10% normal goat serum (Dako, Carpinteria, CA, USA) to block the endogenous peroxidase activity and non-specific signals, respectively.
T118 1262-1453 Sentence denotes For antigen detection, an in-house anti-PEDV N antibody, DE-1, at a dilution of 1:1000 in 10% normal goat serum was applied to the slides for 1 h at RT followed by three times wash with TBST.
T119 1454-1752 Sentence denotes The first antibodies were then captured using the polyclonal anti-rabbit/mouse immunoglobulin, EnVision-DAB+ system (Agilent Technologies, Santa Clara, CA, USA) at RT for 1 h and color was developed afterward with 3, 3′-diaminobenzidine (DAB) chromogen (Agilent Technologies, Santa Clara, CA, USA).
T120 1753-1889 Sentence denotes The slides were counterstained with hematoxylin (MUTO, Tokyo, Japan), mounted in Entellan (Merck, Darmstadt, Germany) and cover slipped.
T121 1890-1980 Sentence denotes Positive signals were visualized under an inverted light microscope (Nikon, Tokyo, Japan).