LitCovid-sentences  

PMC:7019868 / 11249-12954 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T79 0-6 Sentence denotes 2.4.3.
T80 7-56 Sentence denotes Growth Kinetics, Viral Titration and Plaque Assay
T81 57-231 Sentence denotes Confluent monolayers of Vero cells were seeded onto six-well plates (5 × 105 cells/well) and infected with each virus at a MOI of 1 and 0.001 at 37 °C for 1 h in triplicates.
T82 232-342 Sentence denotes The cells were then washed twice with Dulbecco’s phosphate-buffered saline (DPBS) and maintained in PI medium.
T83 343-540 Sentence denotes The supernatants at indicated time points were collected and proceeded for viral quantification on Vero cells in 96-well plates using the standard 50% tissue-culture infectious dose (TCID50) assay.
T84 541-744 Sentence denotes In brief, Vero cells in 96-well plates were washed twice with DPBS and then incubated with a 10-fold serially diluted culture supernatant acquired from the aforementioned six-well plate at 37 °C for 1 h.
T85 745-846 Sentence denotes After absorption, the inoculum was removed and replaced with fresh PI-medium following one wash step.
T86 847-931 Sentence denotes The titers were determined at 72 h post-infection using the Reed–Muench method [21].
T87 932-1026 Sentence denotes Plaque assays were performed as previously described [19] to characterize plaque morphologies.
T88 1027-1244 Sentence denotes Briefly, after absorption of PEDVs at an MOI of 0.0001, confluent monolayers of Vero cells in six-well plates were washed twice with DPBS and then covered with an overlay of pre-warmed PI medium containing 1% agarose.
T89 1245-1355 Sentence denotes After solidification of the overlays, the plates were incubated at 37 °C for 72 h to produce distinct plaques.
T90 1356-1464 Sentence denotes The cells were then fixed in 3.16% neutral-buffered formalin for 1 h before removing the semisolid overlays.
T91 1465-1557 Sentence denotes The plates were stained with 1% crystal violet in 20% ethanol and distilled water for 1 min.
T92 1558-1705 Sentence denotes Viral plaques were inspected after washing off the crystal violet solution, rinsing the plates with water, and air-drying at room temperature (RT).