| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T63 |
0-38 |
Sentence |
denotes |
Protocol options and application notes |
| T64 |
39-213 |
Sentence |
denotes |
Laboratories participating in the evaluation used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) with the same oligonucleotide concentrations and cycling conditions. |
| T65 |
214-288 |
Sentence |
denotes |
The QIAGEN One-Step RT-PCR Kit was also tested and found to be compatible. |
| T66 |
289-457 |
Sentence |
denotes |
The intended cross-reactivity of all assays with viral RNA of SARS-CoV allows us to use the assays without having to rely on external sources of specific 2019-nCoV RNA. |
| T67 |
458-604 |
Sentence |
denotes |
For a routine workflow, we recommend the E gene assay as the first-line screening tool, followed by confirmatory testing with the RdRp gene assay. |
| T68 |
605-778 |
Sentence |
denotes |
Application of the RdRp gene assay with dual colour technology can discriminate 2019-nCoV (both probes positive) from SARS-CoV RNA if the latter is used as positive control. |
| T69 |
779-879 |
Sentence |
denotes |
Alternatively, laboratories may choose to run the RdRp assay with only the 2019-nCoV-specific probe. |
