PMC:6988269 / 16254-18622 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T138 0-41 Sentence denotes Cross-reactivity with other coronaviruses
T139 42-222 Sentence denotes Cell culture supernatants containing all endemic human coronaviruses (HCoV)‑229E, ‑NL63, ‑OC43 and ‑HKU1 as well as MERS-CoV were tested in duplicate in all three assays (Table 2).
T140 223-304 Sentence denotes For the non-cultivable HCoV-HKU1, supernatant from human airway culture was used.
T141 305-479 Sentence denotes Viral RNA concentration in all samples was determined by specific real-time RT-PCRs and in vitro-transcribed RNA standards designed for absolute quantification of viral load.
T142 480-585 Sentence denotes Additional undiluted (but not quantified) cell culture supernatants were tested as summarised in Table 2.
T143 586-651 Sentence denotes These were additionally mixed into negative human sputum samples.
T144 652-734 Sentence denotes None of the tested viruses or virus preparations showed reactivity with any assay.
T145 735-921 Sentence denotes Table 2 Tests of known respiratory viruses and bacteria in clinical samples and cell culture preparations for cross-reactivity in 2019 novel coronavirus E and RdRp gene assays (n = 310)
T146 922-993 Sentence denotes Clinical samples with known viruses Clinical samplesa Virus isolatesb
T147 994-1011 Sentence denotes HCoV-HKU1 14 1c
T148 1012-1029 Sentence denotes HCoV-OC43 16 2d
T149 1030-1047 Sentence denotes HCoV-NL63 14 1e
T150 1048-1065 Sentence denotes HCoV-229E 18 2f
T151 1066-1081 Sentence denotes MERS-CoV 5 1g
T152 1082-1111 Sentence denotes Influenza A(H1N1)pdm09 17 1
T153 1112-1136 Sentence denotes Influenza A(H3N2) 16 1
T154 1137-1167 Sentence denotes Influenza A (untyped) 11 NA
T155 1168-1191 Sentence denotes Influenza A(H5N1) 1 1
T156 1192-1215 Sentence denotes Influenza A(H7N9) 0 1
T157 1216-1257 Sentence denotes Influenza B (Victoria or Yamagata) 31 1
T158 1258-1288 Sentence denotes Rhinovirus/enterovirus 31 NA
T159 1289-1330 Sentence denotes Respiratory syncytial virus (A/B) 33 NA
T160 1331-1360 Sentence denotes Parainfluenza 1 virus 12 NA
T161 1361-1390 Sentence denotes Parainfluenza 2 virus 11 NA
T162 1391-1420 Sentence denotes Parainfluenza 3 virus 14 NA
T163 1421-1450 Sentence denotes Parainfluenza 4 virus 11 NA
T164 1451-1480 Sentence denotes Human metapneumovirus 16 NA
T165 1481-1498 Sentence denotes Adenovirus 13 1
T166 1499-1521 Sentence denotes Human bocavirus 6 NA
T167 1522-1537 Sentence denotes Legionella spp.
T168 1539-1544 Sentence denotes 3 NA
T169 1545-1560 Sentence denotes Mycoplasma spp.
T170 1562-1567 Sentence denotes 4 NA
T171 1568-1600 Sentence denotes Total clinical samples 297 NA
T172 1601-1734 Sentence denotes a For samples with multiple viruses detected, the virus with highest concentration is listed, as indicated by real-time PCR Ct value.
T173 1735-1800 Sentence denotes b Directly quantified or spiked in human negative-testing sputum.
T174 1801-1866 Sentence denotes c 1 × 105 RNA copies/mL, determined by specific real-time RT-PCR.
T175 1867-1913 Sentence denotes Isolated from human airway epithelial culture.
T176 1914-1995 Sentence denotes d 1 × 1010 RNA copies/mL, determined by specific real-time RT-PCR of one isolate.
T177 1996-2077 Sentence denotes The other isolate was not quantified but spiked in human negative-testing sputum.
T178 2078-2143 Sentence denotes e 4 × 109 RNA copies/mL, determined by specific real-time RT-PCR.
T179 2144-2224 Sentence denotes f 3 × 109 RNA copies/mL, determined by specific real-time RT-PCR of one isolate.
T180 2225-2302 Sentence denotes The other isolate was not quantified spiked in human negative-testing sputum.
T181 2303-2368 Sentence denotes g 1 × 108 RNA copies/mL, determined by specific real-time RT-PCR.