Id |
Subject |
Object |
Predicate |
Lexical cue |
T138 |
0-41 |
Sentence |
denotes |
Cross-reactivity with other coronaviruses |
T139 |
42-222 |
Sentence |
denotes |
Cell culture supernatants containing all endemic human coronaviruses (HCoV)‑229E, ‑NL63, ‑OC43 and ‑HKU1 as well as MERS-CoV were tested in duplicate in all three assays (Table 2). |
T140 |
223-304 |
Sentence |
denotes |
For the non-cultivable HCoV-HKU1, supernatant from human airway culture was used. |
T141 |
305-479 |
Sentence |
denotes |
Viral RNA concentration in all samples was determined by specific real-time RT-PCRs and in vitro-transcribed RNA standards designed for absolute quantification of viral load. |
T142 |
480-585 |
Sentence |
denotes |
Additional undiluted (but not quantified) cell culture supernatants were tested as summarised in Table 2. |
T143 |
586-651 |
Sentence |
denotes |
These were additionally mixed into negative human sputum samples. |
T144 |
652-734 |
Sentence |
denotes |
None of the tested viruses or virus preparations showed reactivity with any assay. |
T145 |
735-921 |
Sentence |
denotes |
Table 2 Tests of known respiratory viruses and bacteria in clinical samples and cell culture preparations for cross-reactivity in 2019 novel coronavirus E and RdRp gene assays (n = 310) |
T146 |
922-993 |
Sentence |
denotes |
Clinical samples with known viruses Clinical samplesa Virus isolatesb |
T147 |
994-1011 |
Sentence |
denotes |
HCoV-HKU1 14 1c |
T148 |
1012-1029 |
Sentence |
denotes |
HCoV-OC43 16 2d |
T149 |
1030-1047 |
Sentence |
denotes |
HCoV-NL63 14 1e |
T150 |
1048-1065 |
Sentence |
denotes |
HCoV-229E 18 2f |
T151 |
1066-1081 |
Sentence |
denotes |
MERS-CoV 5 1g |
T152 |
1082-1111 |
Sentence |
denotes |
Influenza A(H1N1)pdm09 17 1 |
T153 |
1112-1136 |
Sentence |
denotes |
Influenza A(H3N2) 16 1 |
T154 |
1137-1167 |
Sentence |
denotes |
Influenza A (untyped) 11 NA |
T155 |
1168-1191 |
Sentence |
denotes |
Influenza A(H5N1) 1 1 |
T156 |
1192-1215 |
Sentence |
denotes |
Influenza A(H7N9) 0 1 |
T157 |
1216-1257 |
Sentence |
denotes |
Influenza B (Victoria or Yamagata) 31 1 |
T158 |
1258-1288 |
Sentence |
denotes |
Rhinovirus/enterovirus 31 NA |
T159 |
1289-1330 |
Sentence |
denotes |
Respiratory syncytial virus (A/B) 33 NA |
T160 |
1331-1360 |
Sentence |
denotes |
Parainfluenza 1 virus 12 NA |
T161 |
1361-1390 |
Sentence |
denotes |
Parainfluenza 2 virus 11 NA |
T162 |
1391-1420 |
Sentence |
denotes |
Parainfluenza 3 virus 14 NA |
T163 |
1421-1450 |
Sentence |
denotes |
Parainfluenza 4 virus 11 NA |
T164 |
1451-1480 |
Sentence |
denotes |
Human metapneumovirus 16 NA |
T165 |
1481-1498 |
Sentence |
denotes |
Adenovirus 13 1 |
T166 |
1499-1521 |
Sentence |
denotes |
Human bocavirus 6 NA |
T167 |
1522-1537 |
Sentence |
denotes |
Legionella spp. |
T168 |
1539-1544 |
Sentence |
denotes |
3 NA |
T169 |
1545-1560 |
Sentence |
denotes |
Mycoplasma spp. |
T170 |
1562-1567 |
Sentence |
denotes |
4 NA |
T171 |
1568-1600 |
Sentence |
denotes |
Total clinical samples 297 NA |
T172 |
1601-1734 |
Sentence |
denotes |
a For samples with multiple viruses detected, the virus with highest concentration is listed, as indicated by real-time PCR Ct value. |
T173 |
1735-1800 |
Sentence |
denotes |
b Directly quantified or spiked in human negative-testing sputum. |
T174 |
1801-1866 |
Sentence |
denotes |
c 1 × 105 RNA copies/mL, determined by specific real-time RT-PCR. |
T175 |
1867-1913 |
Sentence |
denotes |
Isolated from human airway epithelial culture. |
T176 |
1914-1995 |
Sentence |
denotes |
d 1 × 1010 RNA copies/mL, determined by specific real-time RT-PCR of one isolate. |
T177 |
1996-2077 |
Sentence |
denotes |
The other isolate was not quantified but spiked in human negative-testing sputum. |
T178 |
2078-2143 |
Sentence |
denotes |
e 4 × 109 RNA copies/mL, determined by specific real-time RT-PCR. |
T179 |
2144-2224 |
Sentence |
denotes |
f 3 × 109 RNA copies/mL, determined by specific real-time RT-PCR of one isolate. |
T180 |
2225-2302 |
Sentence |
denotes |
The other isolate was not quantified spiked in human negative-testing sputum. |
T181 |
2303-2368 |
Sentence |
denotes |
g 1 × 108 RNA copies/mL, determined by specific real-time RT-PCR. |