LitCovid-sentences  

PMC:6988269 / 11187-13620 JSONTXT 12 Projects

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Id Subject Object Predicate Lexical cue
T91 0-51 Sentence denotes Assay sensitivity based on SARS coronavirus virions
T92 52-223 Sentence denotes To obtain a preliminary assessment of analytical sensitivity, we used purified cell culture supernatant containing SARS-CoV strain Frankfurt-1 virions grown on Vero cells.
T93 224-336 Sentence denotes The supernatant was ultrafiltered and thereby concentrated from a ca 20-fold volume of cell culture supernatant.
T94 337-476 Sentence denotes The concentration step simultaneously reduces the relative concentration of background nucleic acids such as not virion-packaged viral RNA.
T95 477-636 Sentence denotes The virion preparation was quantified by real-time RT-PCR using a specific in vitro-transcribed RNA quantification standard as described in Drosten et al. [8].
T96 637-795 Sentence denotes All assays were subjected to replicate testing in order to determine stochastic detection frequencies at each assay’s sensitivity end point (Figure 3A and B).
T97 796-970 Sentence denotes All assays were highly sensitive, with best results obtained for the E gene and RdRp gene assays (5.2 and 3.8 copies per reaction at 95% detection probability, respectively).
T98 971-1023 Sentence denotes These two assays were chosen for further evaluation.
T99 1024-1228 Sentence denotes One of the laboratories participating in the external evaluation used other basic RT-PCR reagents (TaqMan Fast Virus 1-Step Master Mix) and repeated the sensitivity study, with equivalent results (E gene:
T100 1229-1261 Sentence denotes 3.2 RNA copies/reaction (95% CI:
T101 1262-1277 Sentence denotes 2.2–6.8); RdRP:
T102 1278-1310 Sentence denotes 3.7 RNA copies/reaction (95% CI:
T103 1311-1320 Sentence denotes 2.8–8.0).
T104 1321-1485 Sentence denotes Of note, the N gene assay also performed well but was not subjected to intensive further validation because it was slightly less sensitive (Supplementary Figure S2)
T105 1486-1631 Sentence denotes Figure 3 Determination of limits of detection based on SARS coronavirus genomic RNA and 2019 novel coronavirus-specific in vitro transcribed RNA
T106 1632-1714 Sentence denotes CI: confidence intervals; c/r: copies per reaction; IVT: in vitro-transcribed RNA.
T107 1715-1717 Sentence denotes A:
T108 1718-1768 Sentence denotes E gene assay, evaluated with SARS-CoV genomic RNA.
T109 1769-1771 Sentence denotes B:
T110 1772-1824 Sentence denotes RdRp gene assay evaluated with SARS-CoV genomic RNA.
T111 1825-1910 Sentence denotes C: E-gene assay, evaluated with 2019-nCoV-specific in vitro-transcribed RNA standard.
T112 1911-1913 Sentence denotes D:
T113 1914-1998 Sentence denotes RdRp gene assay evaluated with 2019-nCoV-specific in vitro-transcribed RNA standard.
T114 1999-2046 Sentence denotes The x-axis shows input RNA copies per reaction.
T115 2047-2279 Sentence denotes The y-axis shows positive results in all parallel reactions performed, squares are experimental data points resulting from replicate testing of given concentrations (x-axis) in parallels assays (eight replicate reactions per point).
T116 2280-2343 Sentence denotes Technical limits of detection are given in the panels headings.
T117 2344-2398 Sentence denotes The inner line is a probit curve (dose-response rule).
T118 2399-2433 Sentence denotes The outer dotted lines are 95% CI.