| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T240 |
0-210 |
Sentence |
denotes |
Our glycomics-informed glycoproteomics allowed us to assign defined sets of glycans to specific glycosylation sites on 3D-structures of S and ACE2 glycoproteins based on experimental evidence (Figures 4 and 6). |
| T241 |
211-450 |
Sentence |
denotes |
Similar to almost all glycoproteins, microheterogeneity is evident at most glycosylation sites of S and ACE2; each glycosylation site can be modified with one of several glycan structures, generating site-specific glycosylation portfolios. |
| T242 |
451-545 |
Sentence |
denotes |
For modeling purposes, however, explicit structures must be placed at each glycosylation site. |
| T243 |
546-716 |
Sentence |
denotes |
In order to capture the impact of microheterogeneity on S and ACE2 MD we chose to generate glycoforms for modeling that represented reasonable portfolios of glycan types. |
| T244 |
717-998 |
Sentence |
denotes |
Using three glycoform models for S (Abundance, Oxford Class, and Processed) and two models for ACE2 (Abundance, which was equivalent to Oxford Class, and Processed), we generated three MD simulations of the co-complexes of these two glycoproteins (Figure 7; Videos S5, S6, and S7). |
| T245 |
999-1167 |
Sentence |
denotes |
The observed interactions over time allowed us to evaluate glycan-protein contacts between the two proteins and examine potential glycan-glycan interactions (Figure 7). |
