LitCovid-sample-sentences  

PMC:7253482 / 9008-13215 JSONTXT 25 Projects

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Id Subject Object Predicate Lexical cue
T51 0-149 Sentence denotes We subsequently performed glycopeptide analysis to ascertain the compositions of glycans at all of the potential N-linked glycosylation sites (PNGs).
T52 150-366 Sentence denotes MERS, SARS and HKU1 recombinant S proteins were reduced, alkylated and digested with an assortment of proteases to yield glycopeptides, which were subjected to in-line liquid chromatography-mass spectrometry (LC-MS).
T53 367-475 Sentence denotes This revealed differential levels of oligomannose, hybrid, and complex-type glycan populations (Fig. 2a, b).
T54 476-542 Sentence denotes Using structures of the trimeric MERS and SARS S proteins (PDB ID:
T55 543-699 Sentence denotes 5X59 and 5X58, respectively), we generated models of fully glycosylated coronavirus spikes using experimentally determined glycan compositions (Fig. 3a, b).
T56 700-880 Sentence denotes This revealed that oligomannose-type glycans on MERS S co-localize to specific clusters on the head of the S protein, consisting of glycans at Asn155, Asn166, and Asn236 (Fig. 3a).
T57 881-1067 Sentence denotes We hypothesized that the fully oligomannose-type glycan population in this cluster arises due to the hindered accessibility of glycan processing enzymes to access the substrate glycan28.
T58 1068-1174 Sentence denotes As such, we performed mutagenesis to knock out glycosylation sites with N155A, N166A, and N236A mutations.
T59 1175-1347 Sentence denotes Site-specific analysis of these glycan-KO mutants revealed enhanced trimming of mannose residues, i.e. increased processing, when glycan clustering was reduced (SI Fig. 4).
T60 1348-1509 Sentence denotes The presence of clustered oligomannose-type glycans is reminiscent of that found on other viral glycoproteins, including HIV-1 Env and LASV GPC24,31,34,36,45,46.
T61 1510-1618 Sentence denotes Fig. 2 Quantitative site-specific N-linked glycan analysis of SARS and MERS coronavirus spike glycoproteins.
T62 1619-1763 Sentence denotes Purified (a) MERS and b SARS S proteins were digested. Quantitative site-specific N-linked glycan analysis of a MERS and b SARS S glycoproteins.
T63 1764-1913 Sentence denotes Purified S proteins were digested with trypsin, chymotrypsin, alpha-lytic protease, Glu-C, and trypsin plus chymotrypsin, then analysed by LC-ESI MS.
T64 1914-2034 Sentence denotes Glycan compositions are based on the glycan library generated from negative-ion mass spectrometry of released N-glycans.
T65 2035-2386 Sentence denotes The bar graphs represent the relative quantities of each glycan group with oligomannose-type glycan series (M9 to M5; Man9GlcNAc2 to Man5GlcNAc2) (green), afucosylated and fucosylated hybrid glycans (Hybrid & F Hybrid) (dashed pink), and complex glycans grouped according to the number of antennae and presence of core fucosylation (A1 to FA4) (pink).
T66 2387-2436 Sentence denotes Left to right; least processed to most processed.
T67 2437-2498 Sentence denotes The pie charts summarise the quantification of these glycans.
T68 2499-2621 Sentence denotes Additional compositional information regarding the distribution of fucosylation and sialylation can be found in SI Fig. 3.
T69 2622-2701 Sentence denotes Fig. 3 Structure-based mapping of N-linked glycans on MERS and SARS S proteins.
T70 2702-2961 Sentence denotes The modelling of the experimentally observed glycosylation is illustrated on the prefusion structure of trimeric a MERS S (PDB ID 5X59)11 and b SARS S (PDB ID 5X58)11 glycoproteins. Structural-based mapping of N-linked glycans on a MERS and b SARS S proteins.
T71 2962-3139 Sentence denotes The modelling of the experimentally observed glycosylation is illustrated on the prefusion structure of trimeric MERS S (PDB ID 5X59)11 and SARS S (PDB ID 5X58)11 glycoproteins.
T72 3140-3221 Sentence denotes The glycans are colored according to oligomannose content, as defined by the key.
T73 3222-3343 Sentence denotes DPP4 receptor-binding sites and ACE2 receptor-binding sites for MERS and SARS, respectively, are indicated in light blue.
T74 3344-3418 Sentence denotes The S1 and S2 subunits are colored light grey and dark grey, respectively.
T75 3419-3490 Sentence denotes Fig. 4 Amino-acid sequence diversification across SARS and MERS spikes.
T76 3491-3548 Sentence denotes a Amino-acid diversity in SARS and MERS S gene sequences.
T77 3549-3740 Sentence denotes Averaged values for each domain are also shown below. b Comparison of dN/dS values between buried and exposed residues across SARS and MERS S (n = 70 and 100 for SARS and MERS, respectively).
T78 3741-3999 Sentence denotes The error bars correspond to the 95% highest posterior density intervals while the circles indicate mean dN/dS values. c Mapping of the per residue amino-acid diversity shown in A onto the structures of SARS and MERS S (PDB ID 5X58 and 5X59, respectively)11.
T79 4000-4100 Sentence denotes S proteins are presented as backbone traces with residues colored according to amino-acid diversity.
T80 4101-4207 Sentence denotes Residues with elevated diversity are colored in red, and N-linked glycans are presented as white surfaces.