| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T206 |
0-39 |
Sentence |
denotes |
Cryo-EM data analysis and visualization |
| T207 |
40-171 |
Sentence |
denotes |
Single-particle cryo-EM data analysis of BG505 SOSIP.664 in complex with RM20A3 Fab was reproduced directly from Berndsen et al.51. |
| T208 |
172-398 |
Sentence |
denotes |
Data for the SARS-CoV S 2P ectodomain was previously published52 and the final particle stack and alignment parameters from the published reconstruction were used for 3D variability analysis in the SPARX software package76,77. |
| T209 |
399-533 |
Sentence |
denotes |
All metadata for these reconstructions along with raw data images and FSC resolution curves can be found in the original publications. |
| T210 |
534-691 |
Sentence |
denotes |
In summary, both datasets were acquired on a FEI Titan Krios (Thermo Fisher) operating at 300 KeV equipped with a K2 Summit Direct Electron Detector (Gatan). |
| T211 |
692-804 |
Sentence |
denotes |
Movie micrographs were aligned and dose weighted with MotionCor278 and CTF estimation was performed with Gctf79. |
| T212 |
805-890 |
Sentence |
denotes |
Single-particle data processing was performed using CryoSparc v.280 and Relion v.381. |
| T213 |
891-976 |
Sentence |
denotes |
Maps were low-pass filtered using a Gaussian kernel and visualized in UCSF chimera82. |
| T214 |
977-1143 |
Sentence |
denotes |
A detailed description of the auto-thresholding method used to set the isosurface value for visualisation of low-pass filtered maps can be found in Berndsen et al.51. |
