| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T155 |
0-41 |
Sentence |
denotes |
Release and labelling of N-linked glycans |
| T156 |
42-163 |
Sentence |
denotes |
Excised coronavirus S gel bands were washed alternately with acetonitrile and water before drying in a vacuum centrifuge. |
| T157 |
164-258 |
Sentence |
denotes |
The bands were rehydrated with 100 μL of water and incubated with PNGase F at 37 °C overnight. |
| T158 |
259-521 |
Sentence |
denotes |
Aliquots of released N-linked glycans were also fluorescently labelled with procainamide, by adding 100 μL of labelling mixture (110 mg/mL procainamide and 60 mg/mL sodium cyanoborohydrate in 70% DMSO and 30% glacial acetic acid) and incubating for 4 h at 65 °C. |
| T159 |
522-617 |
Sentence |
denotes |
Procainamide labelled glycans were purified using Spe-ed Amide 2 columns (Applied Separations). |
