LitCoin-sentences  

PubMed:28068934 JSONTXT 25 Projects

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Id Subject Object Predicate Lexical cue
T1 0-130 Sentence denotes Concordance between PIK3CA mutations in endoscopic biopsy and surgically resected specimens of esophageal squamous cell carcinoma.
T2 131-255 Sentence denotes BACKGROUND: PIK3CA mutations are expected to be potential therapeutic targets for esophageal squamous cell carcinoma (ESCC).
T3 256-405 Sentence denotes We aimed to clarify the concordance between PIK3CA mutations detected in endoscopic biopsy specimens and corresponding surgically resected specimens.
T4 406-414 Sentence denotes METHODS:
T5 415-779 Sentence denotes We examined five hotspot mutations in the PIK3CA gene (E542K, E545K, E546K, H1047R, and H1047L) in formalin-fixed and paraffin-embedded tissue sections of paired endoscopic biopsy and surgically resected specimens from 181 patients undergoing curative resection for ESCC between 2000 and 2011 using a Luminex technology-based multiplex gene mutation detection kit.
T6 780-788 Sentence denotes RESULTS:
T7 789-913 Sentence denotes Mutation analyses were successfully performed for both endoscopic biopsy and surgically resected specimens in all the cases.
T8 914-1015 Sentence denotes A PIK3CA mutation was detected in either type of specimen in 13 cases (7.2%, 95% confidence interval:
T9 1016-1026 Sentence denotes 3.9-12.0).
T10 1027-1185 Sentence denotes The overall concordance rate, positive predictive value, and negative predictive value were 98.3% (178/181), 90.9% (10/11), and 98.8% (168/170), respectively.
T11 1186-1320 Sentence denotes Among patients with a PIK3CA mutation detected in both types of specimens, the concordance between PIK3CA mutation genotypes was 100%.
T12 1321-1621 Sentence denotes There were three cases with a discordant mutation status between the types of specimens (PIK3CA mutation in surgically resected specimen and wild-type in biopsy specimen in two cases, and the opposite pattern in one case), suggesting possible intratumoral heterogeneity in the PIK3CA mutation status.
T13 1622-1634 Sentence denotes CONCLUSIONS:
T14 1635-1883 Sentence denotes The PIK3CA mutation status was highly concordant between endoscopic biopsy and surgically resected specimens from the same patient, suggesting that endoscopic biopsy specimens can be clinically used to detect PIK3CA mutations in patients with ESCC.