CORD-19:e93a1e03d60bbd5bf79f34cec900d7f2a515a74c JSONTXT 8 Projects

Journal of Infection The index case of SARS-CoV-2 in Scotland: a case report Abstract Since its identification in December 2019, SARS-CoV-2 has infected 125,048 persons globally with cases identified in 118 countries across all continents (1). We report on the Scottish index case of SARS-CoV-2 infection, the virus causing COVID-19. The patient, a 51-year-old male, contacted the Scottish telehealth service for advice on day +1 with a 24-hour history of fever and cough having returned to Scotland from northern Italy on day -2. His symptoms were in keeping with a "possible" case of COVID-19 according to the national case definition at the time. Community SARS-CoV-2 testing was arranged for day +2. The patient had travelled to Italy on day -9 to watch a rugby match in Rome. He travelled with his partner and two friends in a private rental vehicle through Lombardy, Veneto and Tuscany, staying in private rental accommodation, before flying back to Scotland from Milan on day -2. He was not aware of any contact with cases of COVID-19. On day 0 he developed fever, myalgia, malaise and sinusitis. This progressed to a cough productive of green sputum on day +1. His fever had subsided by day +2. The patient remained in self isolation in the community whilst awaiting the results of SARS-CoV-2 PCR which was performed in the West of Scotland Specialist Virology Centre on a combined nose/throat swab. This sample tested positive for SARS-CoV-2 on day +3 with a threshold cycle (Ct) value of 36. An urgent teleconference was held between virology, public health, Scottish ambulance service and the receiving regional High Consequence Infectious Diseases (HCID) unit. The patient was subsequently transferred by Special Operations Response Team (SORT) ambulance to the HCID unit. The patient was escorted from the ambulance to a laminar flow room (with antechamber) on the first floor of the unit by medical staff in appropriate personal protective equipment (PPE). The patient was wearing a surgical face mask throughout transit. The ambulance staff returned to their base for doffing of PPE. The patient was usually well and exercised regularly. He was a lifelong non-smoker. His medical history consisted only of hypertension. He had no known underlying lung disease. He took an ACE-inhibitor and selfmedicated with paracetamol and non-steroidal anti-inflammatory drugs during his illness. On admission, the patient was afebrile with a mild non-productive cough. He had no limitation in exercise tolerance during this acute illness and continued to exercise in his home whilst self-isolating. On assessment, lung fields were clear on auscultation and bilateral scleral injection was noted. Admission vital signs were: heart rate 79 beats per minute, blood pressure 171/105mmHg, respiratory rate 18, pulse oximetry 96% on air and temperature 36.5. The results of routine laboratory parameters are shown in Table 1 . By day three of admission the patient had lymphocytopenia, which was found in 80.4% of patients with laboratory-confirmed non-severe COVID-19 in mainland China (3). Chest radiography was not clinically indicated. PCR testing for other respiratory viruses, Mycoplasma pneumoniae and Chlamydia pneumoniae on the throat swab was negative. Nose and throat samples for SARS-CoV-2 PCR were obtained daily to monitor viral shedding ( Figure 1 ). SARS-CoV-2 PCR on urine, faeces and EDTA blood was negative. The patient was clinically well throughout the admission; no supportive care was required. He was discharged after 8 days, following two sequential nose and throat swabs negative for SARS-CoV-2 by PCR. The initial viral sample was sequenced by the MRC-University of Glasgow Centre for Virus Research, mapped against the Wuhan-Hu-1 reference genome and aligned with a collection of global SARS-CoV-2 sequences, with the resulting analysis released open access. Phylogenetic analysis demonstrated that Scotland/CVR01/2020 belonged to a clade of isolates all acquired in northern Italy (4). Public health undertook contact tracing and testing and no further cases were identified A large case series from China has reported that the majority of COVID-19 cases (81%) had mild disease only, with risk of severe disease and mortality higher in older patients with pre-existing medical conditions (5). The patient described here also had a mild and self-limiting illness. Although asymptomatic and mild manifestations of previous epidemic-associated coronaviruses (SARS and MERS) occur (6,7), these appear to be substantially more common in COVID-19. This poses a challenge for the early identification and isolation of cases, to limit transmission. In the "contain" phase of the UK COVID-19 response, early case identification was reliant on epidemiologic (not severity) criteria. The public health guidance at the time of this case was that travellers to Veneto and Lombardy were considered at risk. The patient had travelled to these areas but in private transport, staying in the area briefly and having limited interactions with residents. Therefore, whilst fulfilling 'at risk' criteria, from a practical perspective the risk seemed low. The kinetics of SARS-CoV-2 viral load have not yet been well characterised in large numbers of patients. Prolonged nasopharyngeal viral shedding was reported in a series of 18 patients from Singapore, with a median of 12 days between first and last positive samples and an initial large decline in viral load followed by a slower decay of residual low-level virus (8) . Similar kinetics were observed in 17 patients from China, where results were stratified by site tested (nose/throat) suggesting quantitatively greater and more prolonged shedding from the nose (9) . The kinetics of shedding in this case match this pattern, with more prolonged nasal shedding (Figure) . National public health guidance on PPE requirements for managing cases of COVID-19 in infectious disease units stipulates use of disposable gowns, two pairs of disposable gloves, visor and FFP3 respirator (10) . These guidelines currently do not recommend use of specific footwear, so it is concerning that SARS-CoV-2 was detected by PCR from the shoe front of a healthcare worker caring for COVID-19 patients (11) . For this case we used disposable boot covers over plastic clogs. As the SARS-CoV-2 outbreak continues, we are learning more about the clinical manifestations of the infection and the logistics of containing and managing cases. This case highlights the need to have a low index of suspicion for diagnosis of COVID-19 and for early isolation. Renal and liver biochemistry values were normal. Note on day 2 a combined throat and nose swab was performed. Negative PCR is plotted as Ct value of 40 (dotted line). KH, CDR, SC, RS conceived of the correspondence. KH and CDR collected the data. KT contributed to the section on virological testing. All authors contributed to the writing of the final version of the article. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.