CORD-19:2011e171a1664541e88ec5333849781130565135 JSONTXT 8 Projects

Abstract positive rates were lower than in other years, and HCoV caused fewer respiratory infections requiring admission, especially in children. While studies on HCoVs with lower respiratory tract infections (LRTIs) have been conducted in other countries, [9] [10] [11] there are no reports on HCoVs affecting LRTIs. In this study, we reviewed clinical presentations of HCoV infection during the 2014 winter season in Korea. This study was performed at Severance Children's Hospital in Seoul, Korea. Outpatient clinic, emergency room, and inpatient ward data were collected. From October 1 to December 31 in 2014, 504 patients under the age of 18 years with episodes of respiratory infection received nasopharyngeal swabs. All clinical data were collected by retrospective review of an electric medical record system. We defined LRTIs as pneumonia or bronchiolitis. When a patient had abnormal lung sounds, such as rales or crackles, with local infiltration or consolidation on chest X-ray, we diagnosed the patient with pneumonia. When lower airway obstruction signs, such as wheezing, decreased lung sounds, or chest retractions, with either a normal or hyperinflation chest X-ray were present, we diagnosed the patient with bronchiolitis. The swab specimens were sent to a virology laboratory in the Department of Laboratory Medicine at Yonsei University Medical Center for respiratory virus detection. DNA or RNA of respiratory viruses was extracted by TANBead Smart LabAssist-32 (BioKett, Taipei, Taiwan). Then the AdvanSure TM Respiratory Virus real-time RT-PCR Kit (LG Life Science, Seoul, Korea) was used to analyze all 504 samples. Using this kit, we were able to detect 14 types of viruses: respiratory syncytial virus (RSV) types A and B, influenza A and B, parainfluenza types 1, 2, and 3, rhinovirus A, metapneumovirus, HCoV-229E, HCoV-OC43, HCoV-NL63, and bocavirus. A total of 504 nasopharyngeal swab samples were included in our study from October 1 to December 31, 2014. The positive rate for respiratory viruses was 67.9% (342 of 504 samples). All samples, except two that were obtained from expectorated sputum, were collected using nasopharyngeal swabs. During the study period, RSV was the most frequently detected virus (48.3%), and HCoV was the third most frequently detected virus (9.7%) regardless of subtype (Table 1 ). The peak incidence of HCoV in our hospital during the study period was during the 48th week of 2014, and this was similar to the pattern shown in the survey of Korean Centers for Disease Control and Prevention (KCDC; http://www.cdc.go.kr/CDC/info/CdcKrInfo0502. jsp?menuIds=HOME001-MNU1175-MNU0048-MNU0050) (Fig. 1) . According to the plot, there was a certain amount of lag time between increases in nationwide HCoV infections and our hospital's pediatric LRTIs due to HCoV. Among the 342 positive samples, co-infection with two viruses was present in 71 samples and three viruses in six samples. When counting the co-infections, samples collected within a 4-week interval with the same result were counted as one result. The most frequent co-infection virus with HCoV was RSV ( Table 2) . The most common signs of HCoV infection were fever and cough. Wheezing increased when combined with RSV. Gastrointestinal symptoms were not common. Most of the patients had an abnormal chest X-ray, such as pneumonic infiltration, ground glass opacity, air trapping, and inter-costal lung herniation. Most of them had a lower respiratory tract infection, such as pneumonia, bronchiolitis, or bronchitis. Antibiotics were used in almost all patients, because the viral panel result was obtained 2-3 days after admission. A nebulizer was less commonly used in the CoV only-infected group. Steroids were used more commonly in the CoV and RSV co-infected groups (Table 3 ). We compared the fever day, obstruction signs (such as wheezing, chest retraction, and nasal flaring), hospital day (HOD), steroid use, and oxygen supply. As RSV infection is the major cause of LRTIs and hospital visits, we compared the selected param- eters between RSV infection and HCoV infection. We did not compare the severity between each subtype of HCoV, because the patient population size was too small. There were no differences among the two groups in terms of fever days, HOD, and oxygen supplementation. Obstruction signs, however, were more prevalent in the RSV only-infection group (51.9%) than in the HCoV-only infected group (25%; p=0.033). The RSV-infected group used more steroids than the HCoV-infected group (p=0.007) ( Table 4 ). As a percentage, HCoV among total respiratory viruses in the winter season was higher in 2014 than in 2013. In 2013, among the total 254 tested samples, 180 samples were positive, and among these, 15 samples were positive for HCoV. In 2014, among the total 504 tested samples, 342 were positive, and among these, 48 samples were positive for HCoV. When these parameters were compared, there were no significant differences between them (Table 5 ). Traditionally, CoV has been considered the most common cause of upper respiratory tract infection (URTI), which causes epidemics every 2-3 years. However, the emergence of SARS, caused by a group II CoV, has provided further insight that this virus can cause severe respiratory distress that is not simply limited to URTIs. In the 2000s, newly identified CoVs, HCoVNL63 and HCoV-HKU1, were introduced. CoV is now an important pathogen in pediatric infections, not only in URTIs but also in LRTIs, such as community-acquired pneumonia and bronchiolitis. [12] [13] [14] [15] Our data revealed a higher positive rate of total respiratory viruses. 16, 17 As our hospital provides tertiary care, the disease severity of admitted children may be higher than other studies. Furthermore, viral study is performed only in children with suspected lower respiratory tract disease. This may have influenced higher detection rate of viruses. Also, a higher positive rate of co-detection of RSV and CoVs was shown. Patterns of co-detection of respiratory virus differ from each study. In our report, patterns did not differ significantly from other reports. Previous studies have reported that influenza virus follows RSV. However, our study showed that CoV seemed to follow RSV, as the influenza season followed an increase in CoV infections during the winter of 2014. Interestingly in this study, there were no differences in fever days or days of hospital stay between RSV and HCoV. RSV is traditionally known as the most common cause of LRTIs in the winter season, especially in bronchiolitis. Since our hospital uses steroids to control obstruction symptoms, RSV showed more obstruction signs and that is why the RSV-infected group used more steroids than the HCoV-infected group. However, we expect that the disease courses of RSV and HCoV were similar in the winter of 2014, because the days of hospital stay and fever days were not very different. When we compared years 2013 and 2014 in terms of HCoV, the parameters we thought could explain the severity of LRTI did not differ. While severity itself due to HCoV was similar, LR-TIs caused by HCoV were much more common in 2014 than in Interestingly, when the data from the Korea CDC showed an increase in HCoV, there was no detected HCoV in our pediatric patients. After about five weeks, HCoV began to be detected in our pediatric patients. It is difficult to determine the reason for the delayed HCoV detection in LRTIs, compared to that in general lower respiratory infections. This study is based on the respiratory virus infection data of the KCDC, which examines references weekly. These references are obtained from the sputum of adults and children who visit primary medical centers due to respiratory infection symptoms, not only for lower but also upper respiratory infection symptoms. This means that those viruses are thought to be the pathogens of the upper respiratory infection, as shown in Fig. 2 . In general, respiratory viruses are spread by children who attend school. They transmit them to adults or infants and young children. In light on this result, we predict that when respiratory infections due to HCoV increase in primary care settings, as reported weekly by the KCDC, lower respiratory infections due to HCoV in children will increase within a few weeks. During the last winter season, HCoV infection was common during the RSV infection period. Traditionally, RSV has been the main cause of bronchiolitis during this season, although, interestingly, HCoV also affected those with a similar diagnosis. Similar to other studies, we found the most common co-infection respiratory virus with HCoV was RSV. 18 According to our data, bronchiolitis with airway obstruction is due to RSV not HCoV. On the other hand, HCoV induces pneumonia rather than bronchiolitis. This result was similar to that of previous studies. 10, 11 Therefore, when caring the lower respiratory tract infection in the season when RSV and HCoV are both common, we should predict the causative virus. If bronchiolitis is the main presentation, RSV should be predicted, and if pneumonia is the main presentation, then HCoV should be predicted. According to our hospital's data, the distribution of HCoV subtype was not different than in other countries, such as the United States and those of Europe. 10, 11 Regardless of the country, HCoV-OC43 and HCoV-NL63 are the main subtypes of HCoV for LRTIs. In the Republic of Korea, respiratory infections due to CoV continue to be present every year. However, since lower respiratory infections due to CoVs were uncommon during the winter of 2014, clinicians did not have much interest in it. As we experienced last winter, CoV can be a pathogen associated with LRTIs of which clinicians should be aware. Although HCoV primarily causes URTIs, we should keep in mind that HCoV can cause both URTIs and LRTIs. In conclusion, infections caused by HCoVs are common and can cause LRTIs. During an epidemic season, clinicians should be given special consideration thereto. When detections of HCoV are increasing, according to reports from the KCDC, pediatricians should keep in mind that LRTI due to HCoV will increase in 4-5 weeks. Also, when combined with other medical conditions, such as neurologic or cardiologic diseases, ICU care may be necessary.