PubMed:7890658 JSONTXT

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":143},"obj":"Sentence"},{"id":"T2","span":{"begin":144,"end":391},"obj":"Sentence"},{"id":"T3","span":{"begin":392,"end":615},"obj":"Sentence"},{"id":"T4","span":{"begin":616,"end":993},"obj":"Sentence"},{"id":"T5","span":{"begin":994,"end":1132},"obj":"Sentence"},{"id":"T6","span":{"begin":1133,"end":1331},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":143},"obj":"Sentence"},{"id":"T2","span":{"begin":144,"end":391},"obj":"Sentence"},{"id":"T3","span":{"begin":392,"end":615},"obj":"Sentence"},{"id":"T4","span":{"begin":616,"end":993},"obj":"Sentence"},{"id":"T5","span":{"begin":994,"end":1132},"obj":"Sentence"},{"id":"T6","span":{"begin":1133,"end":1331},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Identification of human TR2 orphan receptor response element in the transcriptional initiation site of the simian virus 40 major late promoter.\nA DNA response element (TR2RE-SV40) for the TR2 orphan receptor, a member of the steroid-thyroid hormone receptor superfamily, has been identified in the simian virus 40 (SV40) +55 region (nucleotide numbers 368-389, 5'-GTTAAGGTTCGTAGGTCATGGA-3'). Electrophoretic mobility shift assay, using in vitro translated TR2 orphan receptor with a molecular mass of 67 kilodaltons, showed a specific binding with high affinity (dissociation constant = 9 nM) for this DNA sequence. DNA-swap experiments using chloramphenicol acetyl-transferase assay demonstrated that androgen can suppress the transcriptional activities of SV40 early promoter via the interaction between this TR2RE-SV40 and the chimeric receptor AR/TR2/AR with the DNA-binding domain of the TR2 orphan receptor flanked by the N-terminal and androgen-binding domains of the androgen receptor. In addition, this TR2RE-SV40 can function as a repressor to suppress the transcriptional activities of both SV40 early and late promoters. Together, these data suggest the TR2RE-SV40 may represent the first identified natural DNA response element for the TR2 orphan receptor that may function as a repressor for the SV40 gene expression."}

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":233,"end":240},"obj":"http://purl.obolibrary.org/obo/MAT_0000081"}],"text":"Identification of human TR2 orphan receptor response element in the transcriptional initiation site of the simian virus 40 major late promoter.\nA DNA response element (TR2RE-SV40) for the TR2 orphan receptor, a member of the steroid-thyroid hormone receptor superfamily, has been identified in the simian virus 40 (SV40) +55 region (nucleotide numbers 368-389, 5'-GTTAAGGTTCGTAGGTCATGGA-3'). Electrophoretic mobility shift assay, using in vitro translated TR2 orphan receptor with a molecular mass of 67 kilodaltons, showed a specific binding with high affinity (dissociation constant = 9 nM) for this DNA sequence. DNA-swap experiments using chloramphenicol acetyl-transferase assay demonstrated that androgen can suppress the transcriptional activities of SV40 early promoter via the interaction between this TR2RE-SV40 and the chimeric receptor AR/TR2/AR with the DNA-binding domain of the TR2 orphan receptor flanked by the N-terminal and androgen-binding domains of the androgen receptor. In addition, this TR2RE-SV40 can function as a repressor to suppress the transcriptional activities of both SV40 early and late promoters. Together, these data suggest the TR2RE-SV40 may represent the first identified natural DNA response element for the TR2 orphan receptor that may function as a repressor for the SV40 gene expression."}

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":18,"end":60},"obj":"DNA"},{"id":"T2","span":{"begin":68,"end":99},"obj":"DNA"},{"id":"T3","span":{"begin":107,"end":142},"obj":"DNA"},{"id":"T4","span":{"begin":146,"end":166},"obj":"DNA"},{"id":"T5","span":{"begin":168,"end":178},"obj":"DNA"},{"id":"T6","span":{"begin":188,"end":207},"obj":"protein"},{"id":"T7","span":{"begin":225,"end":269},"obj":"protein"},{"id":"T8","span":{"begin":298,"end":331},"obj":"DNA"},{"id":"T9","span":{"begin":333,"end":359},"obj":"DNA"},{"id":"T10","span":{"begin":456,"end":475},"obj":"protein"},{"id":"T11","span":{"begin":501,"end":515},"obj":"protein"},{"id":"T12","span":{"begin":643,"end":677},"obj":"protein"},{"id":"T13","span":{"begin":758,"end":777},"obj":"DNA"},{"id":"T14","span":{"begin":811,"end":821},"obj":"DNA"},{"id":"T15","span":{"begin":830,"end":857},"obj":"protein"},{"id":"T16","span":{"begin":867,"end":885},"obj":"protein"},{"id":"T17","span":{"begin":893,"end":912},"obj":"protein"},{"id":"T18","span":{"begin":928,"end":967},"obj":"protein"},{"id":"T19","span":{"begin":975,"end":992},"obj":"protein"},{"id":"T20","span":{"begin":1012,"end":1022},"obj":"DNA"},{"id":"T21","span":{"begin":1102,"end":1131},"obj":"DNA"},{"id":"T22","span":{"begin":1166,"end":1176},"obj":"DNA"},{"id":"T23","span":{"begin":1220,"end":1240},"obj":"DNA"},{"id":"T24","span":{"begin":1249,"end":1268},"obj":"protein"}],"text":"Identification of human TR2 orphan receptor response element in the transcriptional initiation site of the simian virus 40 major late promoter.\nA DNA response element (TR2RE-SV40) for the TR2 orphan receptor, a member of the steroid-thyroid hormone receptor superfamily, has been identified in the simian virus 40 (SV40) +55 region (nucleotide numbers 368-389, 5'-GTTAAGGTTCGTAGGTCATGGA-3'). Electrophoretic mobility shift assay, using in vitro translated TR2 orphan receptor with a molecular mass of 67 kilodaltons, showed a specific binding with high affinity (dissociation constant = 9 nM) for this DNA sequence. DNA-swap experiments using chloramphenicol acetyl-transferase assay demonstrated that androgen can suppress the transcriptional activities of SV40 early promoter via the interaction between this TR2RE-SV40 and the chimeric receptor AR/TR2/AR with the DNA-binding domain of the TR2 orphan receptor flanked by the N-terminal and androgen-binding domains of the androgen receptor. In addition, this TR2RE-SV40 can function as a repressor to suppress the transcriptional activities of both SV40 early and late promoters. Together, these data suggest the TR2RE-SV40 may represent the first identified natural DNA response element for the TR2 orphan receptor that may function as a repressor for the SV40 gene expression."}

{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":18,"end":60},"obj":"NP"},{"id":"C3","span":{"begin":184,"end":207},"obj":"NP"},{"id":"C2","span":{"begin":144,"end":207},"obj":"NP"},{"id":"C4","span":{"begin":209,"end":269},"obj":"NP"},{"id":"C5","span":{"begin":806,"end":821},"obj":"NP"},{"id":"C6","span":{"begin":889,"end":912},"obj":"NP"},{"id":"C7","span":{"begin":1007,"end":1022},"obj":"NP"},{"id":"C8","span":{"begin":1162,"end":1176},"obj":"NP"},{"id":"C10","span":{"begin":1245,"end":1268},"obj":"NP"},{"id":"C9","span":{"begin":1191,"end":1268},"obj":"NP"},{"id":"C11","span":{"begin":1269,"end":1273},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C2","obj":"C1"},{"id":"R2","pred":"coref-appos","subj":"C4","obj":"C3"},{"id":"R3","pred":"coref-ident","subj":"C5","obj":"C2"},{"id":"R4","pred":"coref-ident","subj":"C6","obj":"C3"},{"id":"R5","pred":"coref-ident","subj":"C7","obj":"C5"},{"id":"R6","pred":"coref-ident","subj":"C8","obj":"C7"},{"id":"R7","pred":"coref-ident","subj":"C10","obj":"C6"},{"id":"R8","pred":"coref-ident","subj":"C9","obj":"C2"},{"id":"R9","pred":"coref-relat","subj":"C11","obj":"C9"}],"text":"Identification of human TR2 orphan receptor response element in the transcriptional initiation site of the simian virus 40 major late promoter.\nA DNA response element (TR2RE-SV40) for the TR2 orphan receptor, a member of the steroid-thyroid hormone receptor superfamily, has been identified in the simian virus 40 (SV40) +55 region (nucleotide numbers 368-389, 5'-GTTAAGGTTCGTAGGTCATGGA-3'). Electrophoretic mobility shift assay, using in vitro translated TR2 orphan receptor with a molecular mass of 67 kilodaltons, showed a specific binding with high affinity (dissociation constant = 9 nM) for this DNA sequence. DNA-swap experiments using chloramphenicol acetyl-transferase assay demonstrated that androgen can suppress the transcriptional activities of SV40 early promoter via the interaction between this TR2RE-SV40 and the chimeric receptor AR/TR2/AR with the DNA-binding domain of the TR2 orphan receptor flanked by the N-terminal and androgen-binding domains of the androgen receptor. In addition, this TR2RE-SV40 can function as a repressor to suppress the transcriptional activities of both SV40 early and late promoters. Together, these data suggest the TR2RE-SV40 may represent the first identified natural DNA response element for the TR2 orphan receptor that may function as a repressor for the SV40 gene expression."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":143},"obj":"Sentence"},{"id":"S2","span":{"begin":144,"end":391},"obj":"Sentence"},{"id":"S3","span":{"begin":392,"end":615},"obj":"Sentence"},{"id":"S4","span":{"begin":616,"end":993},"obj":"Sentence"},{"id":"S5","span":{"begin":994,"end":1132},"obj":"Sentence"},{"id":"S6","span":{"begin":1133,"end":1331},"obj":"Sentence"}],"text":"Identification of human TR2 orphan receptor response element in the transcriptional initiation site of the simian virus 40 major late promoter.\nA DNA response element (TR2RE-SV40) for the TR2 orphan receptor, a member of the steroid-thyroid hormone receptor superfamily, has been identified in the simian virus 40 (SV40) +55 region (nucleotide numbers 368-389, 5'-GTTAAGGTTCGTAGGTCATGGA-3'). Electrophoretic mobility shift assay, using in vitro translated TR2 orphan receptor with a molecular mass of 67 kilodaltons, showed a specific binding with high affinity (dissociation constant = 9 nM) for this DNA sequence. DNA-swap experiments using chloramphenicol acetyl-transferase assay demonstrated that androgen can suppress the transcriptional activities of SV40 early promoter via the interaction between this TR2RE-SV40 and the chimeric receptor AR/TR2/AR with the DNA-binding domain of the TR2 orphan receptor flanked by the N-terminal and androgen-binding domains of the androgen receptor. In addition, this TR2RE-SV40 can function as a repressor to suppress the transcriptional activities of both SV40 early and late promoters. Together, these data suggest the TR2RE-SV40 may represent the first identified natural DNA response element for the TR2 orphan receptor that may function as a repressor for the SV40 gene expression."}

{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":18,"end":43},"obj":"protein_molecule"},{"id":"T2","span":{"begin":68,"end":99},"obj":"DNA_domain_or_region"},{"id":"T3","span":{"begin":107,"end":122},"obj":"virus"},{"id":"T4","span":{"begin":146,"end":166},"obj":"DNA_domain_or_region"},{"id":"T5","span":{"begin":168,"end":178},"obj":"DNA_domain_or_region"},{"id":"T6","span":{"begin":188,"end":207},"obj":"protein_molecule"},{"id":"T7","span":{"begin":225,"end":269},"obj":"protein_family_or_group"},{"id":"T8","span":{"begin":298,"end":313},"obj":"virus"},{"id":"T9","span":{"begin":315,"end":319},"obj":"virus"},{"id":"T10","span":{"begin":333,"end":359},"obj":"DNA_domain_or_region"},{"id":"T11","span":{"begin":361,"end":389},"obj":"polynucleotide"},{"id":"T12","span":{"begin":392,"end":428},"obj":"other_name"},{"id":"T13","span":{"begin":456,"end":475},"obj":"protein_molecule"},{"id":"T14","span":{"begin":501,"end":515},"obj":"protein_molecule"},{"id":"T15","span":{"begin":616,"end":636},"obj":"other_name"},{"id":"T16","span":{"begin":643,"end":677},"obj":"protein_molecule"},{"id":"T17","span":{"begin":702,"end":710},"obj":"lipid"},{"id":"T18","span":{"begin":728,"end":754},"obj":"other_name"},{"id":"T19","span":{"begin":758,"end":762},"obj":"virus"},{"id":"T20","span":{"begin":811,"end":817},"obj":"protein_domain_or_region"},{"id":"T21","span":{"begin":817,"end":821},"obj":"virus"},{"id":"T22","span":{"begin":830,"end":857},"obj":"protein_molecule"},{"id":"T23","span":{"begin":867,"end":885},"obj":"protein_domain_or_region"},{"id":"T24","span":{"begin":893,"end":912},"obj":"protein_molecule"},{"id":"T25","span":{"begin":975,"end":992},"obj":"protein_molecule"},{"id":"T26","span":{"begin":1012,"end":1018},"obj":"DNA_domain_or_region"},{"id":"T27","span":{"begin":1018,"end":1022},"obj":"virus"},{"id":"T28","span":{"begin":1067,"end":1093},"obj":"other_name"},{"id":"T29","span":{"begin":1166,"end":1172},"obj":"DNA_domain_or_region"},{"id":"T30","span":{"begin":1172,"end":1176},"obj":"virus"},{"id":"T31","span":{"begin":1220,"end":1240},"obj":"DNA_domain_or_region"},{"id":"T32","span":{"begin":1249,"end":1268},"obj":"protein_molecule"},{"id":"T33","span":{"begin":1310,"end":1314},"obj":"virus"}],"text":"Identification of human TR2 orphan receptor response element in the transcriptional initiation site of the simian virus 40 major late promoter.\nA DNA response element (TR2RE-SV40) for the TR2 orphan receptor, a member of the steroid-thyroid hormone receptor superfamily, has been identified in the simian virus 40 (SV40) +55 region (nucleotide numbers 368-389, 5'-GTTAAGGTTCGTAGGTCATGGA-3'). Electrophoretic mobility shift assay, using in vitro translated TR2 orphan receptor with a molecular mass of 67 kilodaltons, showed a specific binding with high affinity (dissociation constant = 9 nM) for this DNA sequence. DNA-swap experiments using chloramphenicol acetyl-transferase assay demonstrated that androgen can suppress the transcriptional activities of SV40 early promoter via the interaction between this TR2RE-SV40 and the chimeric receptor AR/TR2/AR with the DNA-binding domain of the TR2 orphan receptor flanked by the N-terminal and androgen-binding domains of the androgen receptor. In addition, this TR2RE-SV40 can function as a repressor to suppress the transcriptional activities of both SV40 early and late promoters. Together, these data suggest the TR2RE-SV40 may represent the first identified natural DNA response element for the TR2 orphan receptor that may function as a repressor for the SV40 gene expression."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":18,"end":23},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":107,"end":122},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":174,"end":178},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":298,"end":313},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":315,"end":319},"obj":"OrganismTaxon"},{"id":"T6","span":{"begin":758,"end":762},"obj":"OrganismTaxon"},{"id":"T7","span":{"begin":817,"end":821},"obj":"OrganismTaxon"},{"id":"T8","span":{"begin":1018,"end":1022},"obj":"OrganismTaxon"},{"id":"T9","span":{"begin":1102,"end":1106},"obj":"OrganismTaxon"},{"id":"T10","span":{"begin":1172,"end":1176},"obj":"OrganismTaxon"},{"id":"T11","span":{"begin":1310,"end":1314},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"1891767"},{"id":"A3","pred":"db_id","subj":"T3","obj":"1891767"},{"id":"A4","pred":"db_id","subj":"T4","obj":"1891767"},{"id":"A5","pred":"db_id","subj":"T5","obj":"1891767"},{"id":"A6","pred":"db_id","subj":"T6","obj":"1891767"},{"id":"A7","pred":"db_id","subj":"T7","obj":"1891767"},{"id":"A8","pred":"db_id","subj":"T8","obj":"1891767"},{"id":"A9","pred":"db_id","subj":"T9","obj":"1891767"},{"id":"A10","pred":"db_id","subj":"T10","obj":"1891767"},{"id":"A11","pred":"db_id","subj":"T11","obj":"1891767"}],"text":"Identification of human TR2 orphan receptor response element in the transcriptional initiation site of the simian virus 40 major late promoter.\nA DNA response element (TR2RE-SV40) for the TR2 orphan receptor, a member of the steroid-thyroid hormone receptor superfamily, has been identified in the simian virus 40 (SV40) +55 region (nucleotide numbers 368-389, 5'-GTTAAGGTTCGTAGGTCATGGA-3'). Electrophoretic mobility shift assay, using in vitro translated TR2 orphan receptor with a molecular mass of 67 kilodaltons, showed a specific binding with high affinity (dissociation constant = 9 nM) for this DNA sequence. DNA-swap experiments using chloramphenicol acetyl-transferase assay demonstrated that androgen can suppress the transcriptional activities of SV40 early promoter via the interaction between this TR2RE-SV40 and the chimeric receptor AR/TR2/AR with the DNA-binding domain of the TR2 orphan receptor flanked by the N-terminal and androgen-binding domains of the androgen receptor. In addition, this TR2RE-SV40 can function as a repressor to suppress the transcriptional activities of both SV40 early and late promoters. Together, these data suggest the TR2RE-SV40 may represent the first identified natural DNA response element for the TR2 orphan receptor that may function as a repressor for the SV40 gene expression."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":233,"end":240},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000081"}],"text":"Identification of human TR2 orphan receptor response element in the transcriptional initiation site of the simian virus 40 major late promoter.\nA DNA response element (TR2RE-SV40) for the TR2 orphan receptor, a member of the steroid-thyroid hormone receptor superfamily, has been identified in the simian virus 40 (SV40) +55 region (nucleotide numbers 368-389, 5'-GTTAAGGTTCGTAGGTCATGGA-3'). Electrophoretic mobility shift assay, using in vitro translated TR2 orphan receptor with a molecular mass of 67 kilodaltons, showed a specific binding with high affinity (dissociation constant = 9 nM) for this DNA sequence. DNA-swap experiments using chloramphenicol acetyl-transferase assay demonstrated that androgen can suppress the transcriptional activities of SV40 early promoter via the interaction between this TR2RE-SV40 and the chimeric receptor AR/TR2/AR with the DNA-binding domain of the TR2 orphan receptor flanked by the N-terminal and androgen-binding domains of the androgen receptor. In addition, this TR2RE-SV40 can function as a repressor to suppress the transcriptional activities of both SV40 early and late promoters. Together, these data suggest the TR2RE-SV40 may represent the first identified natural DNA response element for the TR2 orphan receptor that may function as a repressor for the SV40 gene expression."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":233,"end":240},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0002046"}],"text":"Identification of human TR2 orphan receptor response element in the transcriptional initiation site of the simian virus 40 major late promoter.\nA DNA response element (TR2RE-SV40) for the TR2 orphan receptor, a member of the steroid-thyroid hormone receptor superfamily, has been identified in the simian virus 40 (SV40) +55 region (nucleotide numbers 368-389, 5'-GTTAAGGTTCGTAGGTCATGGA-3'). Electrophoretic mobility shift assay, using in vitro translated TR2 orphan receptor with a molecular mass of 67 kilodaltons, showed a specific binding with high affinity (dissociation constant = 9 nM) for this DNA sequence. DNA-swap experiments using chloramphenicol acetyl-transferase assay demonstrated that androgen can suppress the transcriptional activities of SV40 early promoter via the interaction between this TR2RE-SV40 and the chimeric receptor AR/TR2/AR with the DNA-binding domain of the TR2 orphan receptor flanked by the N-terminal and androgen-binding domains of the androgen receptor. In addition, this TR2RE-SV40 can function as a repressor to suppress the transcriptional activities of both SV40 early and late promoters. Together, these data suggest the TR2RE-SV40 may represent the first identified natural DNA response element for the TR2 orphan receptor that may function as a repressor for the SV40 gene expression."}