PubMed:31972345 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/31972345","sourcedb":"PubMed","sourceid":"31972345","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/31972345","text":"Human sperm phosphoproteome reveals differential phosphoprotein signatures that regulate human sperm motility.\nHuman sperm motility is essential for fertilization and among pathologies underlying male infertility is asthenozoospermia. Nevertheless, mechanisms regulating sperm motility are not completely unraveled. This work investigates phosphoproteins underlying human sperm motility by using differential phosphoproteomic in two human sperm subpopulations: high (HM) and low (LM) motility, obtained by centrifugation in a density gradient. Phosphoproteomics (HPLC-MS/MS triple TOF), comparing human LM and HM phosphoproteomes, identified 210 phosphopeptides with different abundance that correspond with 119 sperm proteins. Analysis showed that 40% of phosphoproteins in LM spermatozoa are involved in metabolism, (catabolism, protein transport, lipid biosynthesis), 25% in spermatogenesis and sperm function, 8% in immune system and 6% in DNA repair. In HM spermatozoa, 48% of phosphoproteins are related to spermatogenesis and sperm function (motility), whereas 8% are associated to metabolism. GSK3α resulted one of the most abundant phosphoproteins in HM spermatozoa. Western blot confirmed that GSK3α phosphorylation is higher in HM spermatozoa. Summarizing, this study i) identified phosphoproteins in two human spermatozoa populations, ii) supports that human spermatozoa rely in protein phosphorylation, such as GSK3 α, to regulate sperm motility, iv) raises the challenge of using some identified human sperm phosphorylated proteins (GSK3α) as targets to develop into clinically relevant biomarkers. SIGNIFICANCE: Human sperm phosphoproteome analyzed by nano HPLC-MS/MS triple TOF identifies the differential abundance of sperm phosphoproteins in two human sperm populations exhibiting high motility (HM) and/or low motility (LM) that were isolated from normozoospermic healthy donors. Majority of human phosphoproteins found in LM spermatozoa are involved in sperm metabolism (40%), whereas those in HM spermatozoa are associated to spermatogenesis and sperm function, as motility (48%), and only 8% are associated to metabolism. One of the most abundant phosphoproteins found in HM spermatozoa is GSK3α, kinase directly involved in the regulation of sperm motility that was also validated by western blot. The biological relevance of this study is based in the fact that supports that mature human sperm cells rely in protein phosphorylation to efficiently regulate sperm motility and allows identifying those regulatory human sperm phosphoproteins. This work will clearly impacts the human reproductive field as it raises the challenge of consider identified human sperm phosphoproteins, such as GSK3α, as potential biological targets to develop into relevant biomarkers for the human clinic or assisted reproductive technology.","tracks":[{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":6,"end":11},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T2","span":{"begin":6,"end":11},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T3","span":{"begin":95,"end":100},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T4","span":{"begin":95,"end":100},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T5","span":{"begin":117,"end":122},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T6","span":{"begin":117,"end":122},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T7","span":{"begin":196,"end":200},"obj":"http://purl.obolibrary.org/obo/MAT_0000029"},{"id":"T8","span":{"begin":271,"end":276},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T9","span":{"begin":271,"end":276},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T10","span":{"begin":372,"end":377},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T11","span":{"begin":372,"end":377},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T12","span":{"begin":439,"end":444},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T13","span":{"begin":439,"end":444},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T14","span":{"begin":712,"end":717},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T15","span":{"begin":712,"end":717},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T16","span":{"begin":898,"end":903},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T17","span":{"begin":898,"end":903},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T18","span":{"begin":1033,"end":1038},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T19","span":{"begin":1033,"end":1038},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T20","span":{"begin":1444,"end":1449},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T21","span":{"begin":1444,"end":1449},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T22","span":{"begin":1516,"end":1521},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T23","span":{"begin":1516,"end":1521},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T24","span":{"begin":1633,"end":1638},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T25","span":{"begin":1633,"end":1638},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T26","span":{"begin":1735,"end":1740},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T27","span":{"begin":1735,"end":1740},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T28","span":{"begin":1770,"end":1775},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T29","span":{"begin":1770,"end":1775},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T30","span":{"begin":1973,"end":1978},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T31","span":{"begin":1973,"end":1978},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T32","span":{"begin":2067,"end":2072},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T33","span":{"begin":2067,"end":2072},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T34","span":{"begin":2265,"end":2270},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T35","span":{"begin":2265,"end":2270},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T36","span":{"begin":2413,"end":2418},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T37","span":{"begin":2413,"end":2418},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T38","span":{"begin":2481,"end":2486},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T39","span":{"begin":2481,"end":2486},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T40","span":{"begin":2542,"end":2547},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T41","span":{"begin":2542,"end":2547},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"T42","span":{"begin":2681,"end":2686},"obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"T43","span":{"begin":2681,"end":2686},"obj":"http://purl.obolibrary.org/obo/MAT_0000218"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T2","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T3","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T4","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T5","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T6","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T7","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T8","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T9","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T10","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T11","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T12","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T13","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T14","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T15","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T16","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T17","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T18","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T19","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T20","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T21","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T22","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T23","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T24","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T25","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T26","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T27","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T28","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T29","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T30","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T31","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T32","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T33","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T34","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T35","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T36","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T37","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T38","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T39","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T40","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T41","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T42","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T43","pred":"source","obj":"Glycosmos6-MAT"}]},{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":110},"obj":"Sentence"},{"id":"T2","span":{"begin":111,"end":234},"obj":"Sentence"},{"id":"T3","span":{"begin":235,"end":315},"obj":"Sentence"},{"id":"T4","span":{"begin":316,"end":543},"obj":"Sentence"},{"id":"T5","span":{"begin":544,"end":727},"obj":"Sentence"},{"id":"T6","span":{"begin":728,"end":955},"obj":"Sentence"},{"id":"T7","span":{"begin":956,"end":1100},"obj":"Sentence"},{"id":"T8","span":{"begin":1101,"end":1175},"obj":"Sentence"},{"id":"T9","span":{"begin":1176,"end":1254},"obj":"Sentence"},{"id":"T10","span":{"begin":1255,"end":1612},"obj":"Sentence"},{"id":"T11","span":{"begin":1613,"end":1626},"obj":"Sentence"},{"id":"T12","span":{"begin":1627,"end":1898},"obj":"Sentence"},{"id":"T13","span":{"begin":1899,"end":2143},"obj":"Sentence"},{"id":"T14","span":{"begin":2144,"end":2320},"obj":"Sentence"},{"id":"T15","span":{"begin":2321,"end":2564},"obj":"Sentence"},{"id":"T16","span":{"begin":2565,"end":2844},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"},{"subj":"T12","pred":"source","obj":"sentences"},{"subj":"T13","pred":"source","obj":"sentences"},{"subj":"T14","pred":"source","obj":"sentences"},{"subj":"T15","pred":"source","obj":"sentences"},{"subj":"T16","pred":"source","obj":"sentences"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":196,"end":212},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005372"},{"subj":"T1","pred":"source","obj":"mondo_disease"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":0,"end":5},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":89,"end":94},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":111,"end":116},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":366,"end":371},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":433,"end":438},"obj":"OrganismTaxon"},{"id":"T6","span":{"begin":597,"end":602},"obj":"OrganismTaxon"},{"id":"T7","span":{"begin":1316,"end":1321},"obj":"OrganismTaxon"},{"id":"T8","span":{"begin":1365,"end":1370},"obj":"OrganismTaxon"},{"id":"T9","span":{"begin":1510,"end":1515},"obj":"OrganismTaxon"},{"id":"T10","span":{"begin":1627,"end":1632},"obj":"OrganismTaxon"},{"id":"T11","span":{"begin":1764,"end":1769},"obj":"OrganismTaxon"},{"id":"T12","span":{"begin":1911,"end":1916},"obj":"OrganismTaxon"},{"id":"T13","span":{"begin":2407,"end":2412},"obj":"OrganismTaxon"},{"id":"T14","span":{"begin":2536,"end":2541},"obj":"OrganismTaxon"},{"id":"T15","span":{"begin":2600,"end":2605},"obj":"OrganismTaxon"},{"id":"T16","span":{"begin":2675,"end":2680},"obj":"OrganismTaxon"},{"id":"T17","span":{"begin":2795,"end":2800},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9606"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9606"},{"id":"A5","pred":"db_id","subj":"T5","obj":"9606"},{"id":"A6","pred":"db_id","subj":"T6","obj":"9606"},{"id":"A7","pred":"db_id","subj":"T7","obj":"9606"},{"id":"A8","pred":"db_id","subj":"T8","obj":"9606"},{"id":"A9","pred":"db_id","subj":"T9","obj":"9606"},{"id":"A10","pred":"db_id","subj":"T10","obj":"9606"},{"id":"A11","pred":"db_id","subj":"T11","obj":"9606"},{"id":"A12","pred":"db_id","subj":"T12","obj":"9606"},{"id":"A13","pred":"db_id","subj":"T13","obj":"9606"},{"id":"A14","pred":"db_id","subj":"T14","obj":"9606"},{"id":"A15","pred":"db_id","subj":"T15","obj":"9606"},{"id":"A16","pred":"db_id","subj":"T16","obj":"9606"},{"id":"A17","pred":"db_id","subj":"T17","obj":"9606"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"},{"subj":"T4","pred":"source","obj":"NCBITAXON"},{"subj":"T5","pred":"source","obj":"NCBITAXON"},{"subj":"T6","pred":"source","obj":"NCBITAXON"},{"subj":"T7","pred":"source","obj":"NCBITAXON"},{"subj":"T8","pred":"source","obj":"NCBITAXON"},{"subj":"T9","pred":"source","obj":"NCBITAXON"},{"subj":"T10","pred":"source","obj":"NCBITAXON"},{"subj":"T11","pred":"source","obj":"NCBITAXON"},{"subj":"T12","pred":"source","obj":"NCBITAXON"},{"subj":"T13","pred":"source","obj":"NCBITAXON"},{"subj":"T14","pred":"source","obj":"NCBITAXON"},{"subj":"T15","pred":"source","obj":"NCBITAXON"},{"subj":"T16","pred":"source","obj":"NCBITAXON"},{"subj":"T17","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":6,"end":11},"obj":"Body_part"},{"id":"T3","span":{"begin":95,"end":100},"obj":"Body_part"},{"id":"T5","span":{"begin":117,"end":122},"obj":"Body_part"},{"id":"T7","span":{"begin":271,"end":276},"obj":"Body_part"},{"id":"T9","span":{"begin":372,"end":377},"obj":"Body_part"},{"id":"T11","span":{"begin":439,"end":444},"obj":"Body_part"},{"id":"T13","span":{"begin":712,"end":717},"obj":"Body_part"},{"id":"T15","span":{"begin":898,"end":903},"obj":"Body_part"},{"id":"T17","span":{"begin":920,"end":933},"obj":"Body_part"},{"id":"T18","span":{"begin":1033,"end":1038},"obj":"Body_part"},{"id":"T20","span":{"begin":1444,"end":1449},"obj":"Body_part"},{"id":"T22","span":{"begin":1516,"end":1521},"obj":"Body_part"},{"id":"T24","span":{"begin":1633,"end":1638},"obj":"Body_part"},{"id":"T26","span":{"begin":1735,"end":1740},"obj":"Body_part"},{"id":"T28","span":{"begin":1770,"end":1775},"obj":"Body_part"},{"id":"T30","span":{"begin":1973,"end":1978},"obj":"Body_part"},{"id":"T32","span":{"begin":2067,"end":2072},"obj":"Body_part"},{"id":"T34","span":{"begin":2265,"end":2270},"obj":"Body_part"},{"id":"T36","span":{"begin":2413,"end":2418},"obj":"Body_part"},{"id":"T38","span":{"begin":2481,"end":2486},"obj":"Body_part"},{"id":"T40","span":{"begin":2542,"end":2547},"obj":"Body_part"},{"id":"T42","span":{"begin":2681,"end":2686},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A4","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A6","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A8","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A9","pred":"uberon_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A10","pred":"uberon_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A11","pred":"uberon_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A12","pred":"uberon_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A13","pred":"uberon_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A14","pred":"uberon_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A15","pred":"uberon_id","subj":"T15","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A16","pred":"uberon_id","subj":"T15","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A17","pred":"uberon_id","subj":"T17","obj":"http://purl.obolibrary.org/obo/UBERON_0002405"},{"id":"A18","pred":"uberon_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A19","pred":"uberon_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A20","pred":"uberon_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A21","pred":"uberon_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A22","pred":"uberon_id","subj":"T22","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A23","pred":"uberon_id","subj":"T22","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A24","pred":"uberon_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A25","pred":"uberon_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A26","pred":"uberon_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A27","pred":"uberon_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A28","pred":"uberon_id","subj":"T28","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A29","pred":"uberon_id","subj":"T28","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A30","pred":"uberon_id","subj":"T30","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A31","pred":"uberon_id","subj":"T30","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A32","pred":"uberon_id","subj":"T32","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A33","pred":"uberon_id","subj":"T32","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A34","pred":"uberon_id","subj":"T34","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A35","pred":"uberon_id","subj":"T34","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A36","pred":"uberon_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A37","pred":"uberon_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A38","pred":"uberon_id","subj":"T38","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A39","pred":"uberon_id","subj":"T38","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A40","pred":"uberon_id","subj":"T40","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A41","pred":"uberon_id","subj":"T40","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"id":"A42","pred":"uberon_id","subj":"T42","obj":"http://purl.obolibrary.org/obo/CL_0000019"},{"id":"A43","pred":"uberon_id","subj":"T42","obj":"http://purl.obolibrary.org/obo/UBERON_0001968"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T3","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T5","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T7","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T9","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T11","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T13","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T15","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T17","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T18","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T20","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T22","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T24","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T26","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T28","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T30","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T32","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T34","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T36","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T38","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T40","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T42","pred":"source","obj":"Anatomy-UBERON"}]},{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":6,"end":11},"obj":"Body_part"},{"id":"T3","span":{"begin":95,"end":100},"obj":"Body_part"},{"id":"T5","span":{"begin":117,"end":122},"obj":"Body_part"},{"id":"T7","span":{"begin":196,"end":200},"obj":"Body_part"},{"id":"T8","span":{"begin":271,"end":276},"obj":"Body_part"},{"id":"T10","span":{"begin":372,"end":377},"obj":"Body_part"},{"id":"T12","span":{"begin":439,"end":444},"obj":"Body_part"},{"id":"T14","span":{"begin":712,"end":717},"obj":"Body_part"},{"id":"T16","span":{"begin":898,"end":903},"obj":"Body_part"},{"id":"T18","span":{"begin":1033,"end":1038},"obj":"Body_part"},{"id":"T20","span":{"begin":1444,"end":1449},"obj":"Body_part"},{"id":"T22","span":{"begin":1516,"end":1521},"obj":"Body_part"},{"id":"T24","span":{"begin":1633,"end":1638},"obj":"Body_part"},{"id":"T26","span":{"begin":1735,"end":1740},"obj":"Body_part"},{"id":"T28","span":{"begin":1770,"end":1775},"obj":"Body_part"},{"id":"T30","span":{"begin":1973,"end":1978},"obj":"Body_part"},{"id":"T32","span":{"begin":2067,"end":2072},"obj":"Body_part"},{"id":"T34","span":{"begin":2265,"end":2270},"obj":"Body_part"},{"id":"T36","span":{"begin":2413,"end":2418},"obj":"Body_part"},{"id":"T37","span":{"begin":2481,"end":2486},"obj":"Body_part"},{"id":"T39","span":{"begin":2542,"end":2547},"obj":"Body_part"},{"id":"T41","span":{"begin":2681,"end":2686},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A2","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A4","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A5","pred":"mat_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A6","pred":"mat_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A7","pred":"mat_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MAT_0000029"},{"id":"A8","pred":"mat_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A9","pred":"mat_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A10","pred":"mat_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A11","pred":"mat_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A12","pred":"mat_id","subj":"T12","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A13","pred":"mat_id","subj":"T12","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A14","pred":"mat_id","subj":"T14","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A15","pred":"mat_id","subj":"T14","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A16","pred":"mat_id","subj":"T16","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A17","pred":"mat_id","subj":"T16","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A18","pred":"mat_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A19","pred":"mat_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A20","pred":"mat_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A21","pred":"mat_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A22","pred":"mat_id","subj":"T22","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A23","pred":"mat_id","subj":"T22","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A24","pred":"mat_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A25","pred":"mat_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A26","pred":"mat_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A27","pred":"mat_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A28","pred":"mat_id","subj":"T28","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A29","pred":"mat_id","subj":"T28","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A30","pred":"mat_id","subj":"T30","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A31","pred":"mat_id","subj":"T30","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A32","pred":"mat_id","subj":"T32","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A33","pred":"mat_id","subj":"T32","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A34","pred":"mat_id","subj":"T34","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A35","pred":"mat_id","subj":"T34","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A36","pred":"mat_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A37","pred":"mat_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A38","pred":"mat_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A39","pred":"mat_id","subj":"T39","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A40","pred":"mat_id","subj":"T39","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"id":"A41","pred":"mat_id","subj":"T41","obj":"http://purl.obolibrary.org/obo/MAT_0000131"},{"id":"A42","pred":"mat_id","subj":"T41","obj":"http://purl.obolibrary.org/obo/MAT_0000218"},{"subj":"T1","pred":"source","obj":"Anatomy-MAT"},{"subj":"T3","pred":"source","obj":"Anatomy-MAT"},{"subj":"T5","pred":"source","obj":"Anatomy-MAT"},{"subj":"T7","pred":"source","obj":"Anatomy-MAT"},{"subj":"T8","pred":"source","obj":"Anatomy-MAT"},{"subj":"T10","pred":"source","obj":"Anatomy-MAT"},{"subj":"T12","pred":"source","obj":"Anatomy-MAT"},{"subj":"T14","pred":"source","obj":"Anatomy-MAT"},{"subj":"T16","pred":"source","obj":"Anatomy-MAT"},{"subj":"T18","pred":"source","obj":"Anatomy-MAT"},{"subj":"T20","pred":"source","obj":"Anatomy-MAT"},{"subj":"T22","pred":"source","obj":"Anatomy-MAT"},{"subj":"T24","pred":"source","obj":"Anatomy-MAT"},{"subj":"T26","pred":"source","obj":"Anatomy-MAT"},{"subj":"T28","pred":"source","obj":"Anatomy-MAT"},{"subj":"T30","pred":"source","obj":"Anatomy-MAT"},{"subj":"T32","pred":"source","obj":"Anatomy-MAT"},{"subj":"T34","pred":"source","obj":"Anatomy-MAT"},{"subj":"T36","pred":"source","obj":"Anatomy-MAT"},{"subj":"T37","pred":"source","obj":"Anatomy-MAT"},{"subj":"T39","pred":"source","obj":"Anatomy-MAT"},{"subj":"T41","pred":"source","obj":"Anatomy-MAT"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Glycosmos6-MAT","color":"#e393ec","default":true},{"id":"sentences","color":"#93ecc9"},{"id":"mondo_disease","color":"#ecaf93"},{"id":"NCBITAXON","color":"#9593ec"},{"id":"Anatomy-UBERON","color":"#aaec93"},{"id":"Anatomy-MAT","color":"#ec93c4"}]}]}}