PubMed:28871180 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/28871180","sourcedb":"PubMed","sourceid":"28871180","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/28871180","text":"Phenotyping and susceptibility of established porcine cells lines to African Swine Fever Virus infection and viral production.\nAfrican swine fever virus (ASFV) is a highly pathogenic, double-stranded DNA virus with a marked tropism for cells of the monocyte-macrophage lineage, affecting swine species and provoking severe economic losses and health threats. In the present study, four established porcine cell lines, IPAM-WT, IPAM-CD163, C∆2+ and WSL, were compared to porcine alveolar macrophage (PAM) in terms of surface marker phenotype, susceptibility to ASFV infection and virus production. The virulent ASFV Armenia/07, E70 or the naturally attenuated NHV/P68 strains were used as viral models. Cells expressed only low levels of specific receptors linked to the monocyte/macrophage lineage, with low levels of infection overall, with the exception of WSL, which showed more efficient production of strain NHV/P68 but not of strains E70 and 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