PubMed:26231571
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/26231571","sourcedb":"PubMed","sourceid":"26231571","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/26231571","text":"Purification and Initial Functions of Sex-Specific Storage Protein 2 in Bombyx mori.\nIn this study, we identified a heat-resistant protein from the chrysalis stage of the silkworm which we named sex-specific storage protein 2 (SSP2). This protein was stable even at 80 °C, and has an amino acid sequence that is 90.65 % homologous to SP2. We utilized the heat-resistant characteristics of SSP2 to purify the protein and maintain its biological activity. In addition, using flow cytometry and the MTT assay, we found that SSP2 had anti-apoptotic effects on BmN cells, and that SSP2 could also inhibit cell apoptosis induced by chemical factors. These results suggest that SSP2 has a cell-protective function, and provides a basis for future work on the function of storage proteins in silkworm.","tracks":[{"project":"silkworm_test","denotations":[{"id":null,"span":{"begin":38,"end":68},"obj":"Gene:733005"},{"id":null,"span":{"begin":72,"end":83},"obj":"Species:7091"},{"id":null,"span":{"begin":171,"end":179},"obj":"Species:7091"},{"id":null,"span":{"begin":195,"end":225},"obj":"Gene:733005"},{"id":null,"span":{"begin":227,"end":231},"obj":"Gene:733005"},{"id":null,"span":{"begin":334,"end":337},"obj":"Gene:733005"},{"id":null,"span":{"begin":389,"end":393},"obj":"Gene:733005"},{"id":null,"span":{"begin":496,"end":499},"obj":"Chemical:MESH:C070243"},{"id":null,"span":{"begin":521,"end":525},"obj":"Gene:733005"},{"id":null,"span":{"begin":576,"end":580},"obj":"Gene:733005"},{"id":null,"span":{"begin":671,"end":675},"obj":"Gene:733005"},{"id":null,"span":{"begin":784,"end":792},"obj":"Species:7091"}],"attributes":[{"subj":null,"pred":"source","obj":"silkworm_test"},{"subj":null,"pred":"source","obj":"silkworm_test"},{"subj":null,"pred":"source","obj":"silkworm_test"},{"subj":null,"pred":"source","obj":"silkworm_test"},{"subj":null,"pred":"source","obj":"silkworm_test"},{"subj":null,"pred":"source","obj":"silkworm_test"},{"subj":null,"pred":"source","obj":"silkworm_test"},{"subj":null,"pred":"source","obj":"silkworm_test"},{"subj":null,"pred":"source","obj":"silkworm_test"},{"subj":null,"pred":"source","obj":"silkworm_test"},{"subj":null,"pred":"source","obj":"silkworm_test"},{"subj":null,"pred":"source","obj":"silkworm_test"}]},{"project":"silkworm_phenotype","denotations":[{"id":"T9","span":{"begin":521,"end":525},"obj":"Gene:733005"},{"id":"T8","span":{"begin":496,"end":499},"obj":"Chemical:MESH:C070243"},{"id":"T7","span":{"begin":389,"end":393},"obj":"Gene:733005"},{"id":"T6","span":{"begin":334,"end":337},"obj":"Gene:733005"},{"id":"T5","span":{"begin":227,"end":231},"obj":"Gene:733005"},{"id":"T4","span":{"begin":195,"end":225},"obj":"Gene:733005"},{"id":"T3","span":{"begin":171,"end":179},"obj":"Species:7091"},{"id":"T21","span":{"begin":51,"end":68},"obj":"BMO_00472"},{"id":"T20","span":{"begin":208,"end":225},"obj":"BMO_00472"},{"id":"T2","span":{"begin":72,"end":83},"obj":"Species:7091"},{"id":"T19","span":{"begin":227,"end":231},"obj":"BMO_00472"},{"id":"T17","span":{"begin":334,"end":337},"obj":"BMO_00472"},{"id":"T16","span":{"begin":389,"end":393},"obj":"BMO_00472"},{"id":"T15","span":{"begin":521,"end":525},"obj":"BMO_00472"},{"id":"T14","span":{"begin":576,"end":580},"obj":"BMO_00472"},{"id":"T13","span":{"begin":671,"end":675},"obj":"BMO_00472"},{"id":"T12","span":{"begin":784,"end":792},"obj":"Species:7091"},{"id":"T11","span":{"begin":671,"end":675},"obj":"Gene:733005"},{"id":"T10","span":{"begin":576,"end":580},"obj":"Gene:733005"},{"id":"T1","span":{"begin":38,"end":68},"obj":"Gene:733005"}],"attributes":[{"subj":"T9","pred":"source","obj":"silkworm_phenotype"},{"subj":"T8","pred":"source","obj":"silkworm_phenotype"},{"subj":"T7","pred":"source","obj":"silkworm_phenotype"},{"subj":"T6","pred":"source","obj":"silkworm_phenotype"},{"subj":"T5","pred":"source","obj":"silkworm_phenotype"},{"subj":"T4","pred":"source","obj":"silkworm_phenotype"},{"subj":"T3","pred":"source","obj":"silkworm_phenotype"},{"subj":"T21","pred":"source","obj":"silkworm_phenotype"},{"subj":"T20","pred":"source","obj":"silkworm_phenotype"},{"subj":"T2","pred":"source","obj":"silkworm_phenotype"},{"subj":"T19","pred":"source","obj":"silkworm_phenotype"},{"subj":"T17","pred":"source","obj":"silkworm_phenotype"},{"subj":"T16","pred":"source","obj":"silkworm_phenotype"},{"subj":"T15","pred":"source","obj":"silkworm_phenotype"},{"subj":"T14","pred":"source","obj":"silkworm_phenotype"},{"subj":"T13","pred":"source","obj":"silkworm_phenotype"},{"subj":"T12","pred":"source","obj":"silkworm_phenotype"},{"subj":"T11","pred":"source","obj":"silkworm_phenotype"},{"subj":"T10","pred":"source","obj":"silkworm_phenotype"},{"subj":"T1","pred":"source","obj":"silkworm_phenotype"}]},{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":38,"end":68},"obj":"Gene:733005"},{"id":"T2","span":{"begin":72,"end":83},"obj":"Species:7091"},{"id":"T3","span":{"begin":171,"end":179},"obj":"Species:7091"},{"id":"T4","span":{"begin":195,"end":225},"obj":"Gene:733005"},{"id":"T5","span":{"begin":227,"end":231},"obj":"Gene:733005"},{"id":"T6","span":{"begin":334,"end":337},"obj":"Gene:733005"},{"id":"T7","span":{"begin":389,"end":393},"obj":"Gene:733005"},{"id":"T8","span":{"begin":496,"end":499},"obj":"Chemical:MESH:C070243"},{"id":"T9","span":{"begin":521,"end":525},"obj":"Gene:733005"},{"id":"T10","span":{"begin":576,"end":580},"obj":"Gene:733005"},{"id":"T11","span":{"begin":671,"end":675},"obj":"Gene:733005"},{"id":"T12","span":{"begin":784,"end":792},"obj":"Species:7091"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"},{"subj":"T6","pred":"source","obj":"silkworm"},{"subj":"T7","pred":"source","obj":"silkworm"},{"subj":"T8","pred":"source","obj":"silkworm"},{"subj":"T9","pred":"source","obj":"silkworm"},{"subj":"T10","pred":"source","obj":"silkworm"},{"subj":"T11","pred":"source","obj":"silkworm"},{"subj":"T12","pred":"source","obj":"silkworm"}]},{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":38,"end":68},"obj":"Gene:733005"},{"id":"T10","span":{"begin":576,"end":580},"obj":"Gene:733005"},{"id":"T11","span":{"begin":671,"end":675},"obj":"Gene:733005"},{"id":"T12","span":{"begin":784,"end":792},"obj":"Species:7091"},{"id":"T13","span":{"begin":671,"end":675},"obj":"BMO_00472"},{"id":"T14","span":{"begin":576,"end":580},"obj":"BMO_00472"},{"id":"T15","span":{"begin":521,"end":525},"obj":"BMO_00472"},{"id":"T16","span":{"begin":389,"end":393},"obj":"BMO_00472"},{"id":"T17","span":{"begin":334,"end":337},"obj":"BMO_00472"},{"id":"T19","span":{"begin":227,"end":231},"obj":"BMO_00472"},{"id":"T2","span":{"begin":72,"end":83},"obj":"Species:7091"},{"id":"T20","span":{"begin":208,"end":225},"obj":"BMO_00472"},{"id":"T21","span":{"begin":51,"end":68},"obj":"BMO_00472"},{"id":"T3","span":{"begin":171,"end":179},"obj":"Species:7091"},{"id":"T4","span":{"begin":195,"end":225},"obj":"Gene:733005"},{"id":"T5","span":{"begin":227,"end":231},"obj":"Gene:733005"},{"id":"T6","span":{"begin":334,"end":337},"obj":"Gene:733005"},{"id":"T7","span":{"begin":389,"end":393},"obj":"Gene:733005"},{"id":"T8","span":{"begin":496,"end":499},"obj":"Chemical:MESH:C070243"},{"id":"T9","span":{"begin":521,"end":525},"obj":"Gene:733005"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkwormbase"},{"subj":"T10","pred":"source","obj":"silkwormbase"},{"subj":"T11","pred":"source","obj":"silkwormbase"},{"subj":"T12","pred":"source","obj":"silkwormbase"},{"subj":"T13","pred":"source","obj":"silkwormbase"},{"subj":"T14","pred":"source","obj":"silkwormbase"},{"subj":"T15","pred":"source","obj":"silkwormbase"},{"subj":"T16","pred":"source","obj":"silkwormbase"},{"subj":"T17","pred":"source","obj":"silkwormbase"},{"subj":"T19","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T20","pred":"source","obj":"silkwormbase"},{"subj":"T21","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T6","pred":"source","obj":"silkwormbase"},{"subj":"T7","pred":"source","obj":"silkwormbase"},{"subj":"T8","pred":"source","obj":"silkwormbase"},{"subj":"T9","pred":"source","obj":"silkwormbase"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"silkworm_test","color":"#93ecc7","default":true},{"id":"silkworm_phenotype","color":"#ecad93"},{"id":"silkworm","color":"#9393ec"},{"id":"silkwormbase","color":"#acec93"}]}]}}