PubMed:26231571 JSONTXT

{"project":"silkworm_test","denotations":[{"id":null,"span":{"begin":38,"end":68},"obj":"Gene:733005"},{"id":null,"span":{"begin":72,"end":83},"obj":"Species:7091"},{"id":null,"span":{"begin":171,"end":179},"obj":"Species:7091"},{"id":null,"span":{"begin":195,"end":225},"obj":"Gene:733005"},{"id":null,"span":{"begin":227,"end":231},"obj":"Gene:733005"},{"id":null,"span":{"begin":334,"end":337},"obj":"Gene:733005"},{"id":null,"span":{"begin":389,"end":393},"obj":"Gene:733005"},{"id":null,"span":{"begin":496,"end":499},"obj":"Chemical:MESH:C070243"},{"id":null,"span":{"begin":521,"end":525},"obj":"Gene:733005"},{"id":null,"span":{"begin":576,"end":580},"obj":"Gene:733005"},{"id":null,"span":{"begin":671,"end":675},"obj":"Gene:733005"},{"id":null,"span":{"begin":784,"end":792},"obj":"Species:7091"}],"text":"Purification and Initial Functions of Sex-Specific Storage Protein 2 in Bombyx mori.\nIn this study, we identified a heat-resistant protein from the chrysalis stage of the silkworm which we named sex-specific storage protein 2 (SSP2). This protein was stable even at 80 °C, and has an amino acid sequence that is 90.65 % homologous to SP2. We utilized the heat-resistant characteristics of SSP2 to purify the protein and maintain its biological activity. In addition, using flow cytometry and the MTT assay, we found that SSP2 had anti-apoptotic effects on BmN cells, and that SSP2 could also inhibit cell apoptosis induced by chemical factors. These results suggest that SSP2 has a cell-protective function, and provides a basis for future work on the function of storage proteins in silkworm."}

{"project":"silkworm_phenotype","denotations":[{"id":"T9","span":{"begin":521,"end":525},"obj":"Gene:733005"},{"id":"T8","span":{"begin":496,"end":499},"obj":"Chemical:MESH:C070243"},{"id":"T7","span":{"begin":389,"end":393},"obj":"Gene:733005"},{"id":"T6","span":{"begin":334,"end":337},"obj":"Gene:733005"},{"id":"T5","span":{"begin":227,"end":231},"obj":"Gene:733005"},{"id":"T4","span":{"begin":195,"end":225},"obj":"Gene:733005"},{"id":"T3","span":{"begin":171,"end":179},"obj":"Species:7091"},{"id":"T21","span":{"begin":51,"end":68},"obj":"BMO_00472"},{"id":"T20","span":{"begin":208,"end":225},"obj":"BMO_00472"},{"id":"T2","span":{"begin":72,"end":83},"obj":"Species:7091"},{"id":"T19","span":{"begin":227,"end":231},"obj":"BMO_00472"},{"id":"T17","span":{"begin":334,"end":337},"obj":"BMO_00472"},{"id":"T16","span":{"begin":389,"end":393},"obj":"BMO_00472"},{"id":"T15","span":{"begin":521,"end":525},"obj":"BMO_00472"},{"id":"T14","span":{"begin":576,"end":580},"obj":"BMO_00472"},{"id":"T13","span":{"begin":671,"end":675},"obj":"BMO_00472"},{"id":"T12","span":{"begin":784,"end":792},"obj":"Species:7091"},{"id":"T11","span":{"begin":671,"end":675},"obj":"Gene:733005"},{"id":"T10","span":{"begin":576,"end":580},"obj":"Gene:733005"},{"id":"T1","span":{"begin":38,"end":68},"obj":"Gene:733005"}],"text":"Purification and Initial Functions of Sex-Specific Storage Protein 2 in Bombyx mori.\nIn this study, we identified a heat-resistant protein from the chrysalis stage of the silkworm which we named sex-specific storage protein 2 (SSP2). This protein was stable even at 80 °C, and has an amino acid sequence that is 90.65 % homologous to SP2. We utilized the heat-resistant characteristics of SSP2 to purify the protein and maintain its biological activity. In addition, using flow cytometry and the MTT assay, we found that SSP2 had anti-apoptotic effects on BmN cells, and that SSP2 could also inhibit cell apoptosis induced by chemical factors. These results suggest that SSP2 has a cell-protective function, and provides a basis for future work on the function of storage proteins in silkworm."}

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":38,"end":68},"obj":"Gene:733005"},{"id":"T2","span":{"begin":72,"end":83},"obj":"Species:7091"},{"id":"T3","span":{"begin":171,"end":179},"obj":"Species:7091"},{"id":"T4","span":{"begin":195,"end":225},"obj":"Gene:733005"},{"id":"T5","span":{"begin":227,"end":231},"obj":"Gene:733005"},{"id":"T6","span":{"begin":334,"end":337},"obj":"Gene:733005"},{"id":"T7","span":{"begin":389,"end":393},"obj":"Gene:733005"},{"id":"T8","span":{"begin":496,"end":499},"obj":"Chemical:MESH:C070243"},{"id":"T9","span":{"begin":521,"end":525},"obj":"Gene:733005"},{"id":"T10","span":{"begin":576,"end":580},"obj":"Gene:733005"},{"id":"T11","span":{"begin":671,"end":675},"obj":"Gene:733005"},{"id":"T12","span":{"begin":784,"end":792},"obj":"Species:7091"}],"text":"Purification and Initial Functions of Sex-Specific Storage Protein 2 in Bombyx mori.\nIn this study, we identified a heat-resistant protein from the chrysalis stage of the silkworm which we named sex-specific storage protein 2 (SSP2). This protein was stable even at 80 °C, and has an amino acid sequence that is 90.65 % homologous to SP2. We utilized the heat-resistant characteristics of SSP2 to purify the protein and maintain its biological activity. In addition, using flow cytometry and the MTT assay, we found that SSP2 had anti-apoptotic effects on BmN cells, and that SSP2 could also inhibit cell apoptosis induced by chemical factors. These results suggest that SSP2 has a cell-protective function, and provides a basis for future work on the function of storage proteins in silkworm."}

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":38,"end":68},"obj":"Gene:733005"},{"id":"T10","span":{"begin":576,"end":580},"obj":"Gene:733005"},{"id":"T11","span":{"begin":671,"end":675},"obj":"Gene:733005"},{"id":"T12","span":{"begin":784,"end":792},"obj":"Species:7091"},{"id":"T13","span":{"begin":671,"end":675},"obj":"BMO_00472"},{"id":"T14","span":{"begin":576,"end":580},"obj":"BMO_00472"},{"id":"T15","span":{"begin":521,"end":525},"obj":"BMO_00472"},{"id":"T16","span":{"begin":389,"end":393},"obj":"BMO_00472"},{"id":"T17","span":{"begin":334,"end":337},"obj":"BMO_00472"},{"id":"T19","span":{"begin":227,"end":231},"obj":"BMO_00472"},{"id":"T2","span":{"begin":72,"end":83},"obj":"Species:7091"},{"id":"T20","span":{"begin":208,"end":225},"obj":"BMO_00472"},{"id":"T21","span":{"begin":51,"end":68},"obj":"BMO_00472"},{"id":"T3","span":{"begin":171,"end":179},"obj":"Species:7091"},{"id":"T4","span":{"begin":195,"end":225},"obj":"Gene:733005"},{"id":"T5","span":{"begin":227,"end":231},"obj":"Gene:733005"},{"id":"T6","span":{"begin":334,"end":337},"obj":"Gene:733005"},{"id":"T7","span":{"begin":389,"end":393},"obj":"Gene:733005"},{"id":"T8","span":{"begin":496,"end":499},"obj":"Chemical:MESH:C070243"},{"id":"T9","span":{"begin":521,"end":525},"obj":"Gene:733005"}],"text":"Purification and Initial Functions of Sex-Specific Storage Protein 2 in Bombyx mori.\nIn this study, we identified a heat-resistant protein from the chrysalis stage of the silkworm which we named sex-specific storage protein 2 (SSP2). This protein was stable even at 80 °C, and has an amino acid sequence that is 90.65 % homologous to SP2. We utilized the heat-resistant characteristics of SSP2 to purify the protein and maintain its biological activity. In addition, using flow cytometry and the MTT assay, we found that SSP2 had anti-apoptotic effects on BmN cells, and that SSP2 could also inhibit cell apoptosis induced by chemical factors. These results suggest that SSP2 has a cell-protective function, and provides a basis for future work on the function of storage proteins in silkworm."}