PubMed:24443562
Annnotations
GlyCosmos6-UBERON
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 108-117 | Body_part | denotes | junctions |
| T2 | 442-447 | Body_part | denotes | joint |
| T4 | 540-544 | Body_part | denotes | cell |
| T5 | 980-989 | Body_part | denotes | junctions |
| T6 | 1084-1093 | Body_part | denotes | junctions |
| T7 | 1379-1387 | Body_part | denotes | junction |
| T8 | 1468-1476 | Body_part | denotes | junction |
| T9 | 1558-1563 | Body_part | denotes | joint |
PubMed_ArguminSci
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T6 | 880-990 | DRI_Approach | denotes | We demonstrate that MutLγ binds DNA with a high affinity and shows a marked preference for Holliday junctions. |
| T7 | 991-1148 | DRI_Outcome | denotes | We also expressed the human MLH1-MLH3 complex and show that preferential binding to Holliday junctions is a conserved capacity of eukaryotic MutLγ complexes. |
| T8 | 1149-1239 | DRI_Outcome | denotes | Specific DNA recognition has never been observed with any other eukaryotic MutL homologue. |
| T9 | 1240-1332 | DRI_Approach | denotes | MutLγ thus represents a new paradigm for the function of the eukaryotic MutL protein family. |
| T10 | 1333-1482 | DRI_Outcome | denotes | We provide insights into the mode of Holliday junction recognition and show that Mlh1-Mlh3 prefers to bind the open unstacked Holliday junction form. |
| T11 | 1483-1608 | DRI_Approach | denotes | This further supports the model where MutLγ is part of a complex acting on joint molecules to generate crossovers in meiosis. |
| T1 | 119-240 | DRI_Challenge | denotes | MutLγ, a heterodimer of the MutL homologues Mlh1 and Mlh3, plays a critical role during meiotic homologous recombination. |
| T2 | 241-503 | DRI_Challenge | denotes | The meiotic function of Mlh3 is fully dependent on the integrity of a putative nuclease motif DQHAX2EX4E, inferring that the anticipated nuclease activity of Mlh1-Mlh3 is involved in the processing of joint molecules to generate crossover recombination products. |
| T3 | 504-713 | DRI_Background | denotes | Although a vast body of genetic and cell biological data regarding Mlh1-Mlh3 is available, mechanistic insights into its function have been lacking due to the unavailability of the recombinant protein complex. |
| T4 | 714-802 | DRI_Approach | denotes | Here we expressed the yeast Mlh1-Mlh3 heterodimer and purified it into near homogeneity. |
| T5 | 803-879 | DRI_Approach | denotes | We show that recombinant MutLγ is a nuclease that nicks double-stranded DNA. |
c_corpus
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T2 | 4-28 | 350141 | denotes | Saccharomyces cerevisiae |
| T5 | 4-28 | 9511 | denotes | Saccharomyces cerevisiae |
| T3 | 4-28 | D012441 | denotes | Saccharomyces cerevisiae |
| T4 | 4-28 | 4932 | denotes | Saccharomyces cerevisiae |
| T9 | 29-33 | CVCL_G669 | denotes | Mlh1 |
| T6 | 29-33 | PR:Q9ZRV4 | denotes | Mlh1 |
| T7 | 29-33 | PR:P38920 | denotes | Mlh1 |
| T8 | 29-33 | PR:Q54KD8 | denotes | Mlh1 |
| T10 | 29-33 | PR:Q9JK91 | denotes | Mlh1 |
| T11 | 29-33 | PR:P97679 | denotes | Mlh1 |
| T12 | 29-33 | PR:Q9P7W6 | denotes | Mlh1 |
| T13 | 29-33 | PR:000010442 | denotes | Mlh1 |
| T14 | 29-33 | PR:P40692 | denotes | Mlh1 |
| T15 | 34-38 | PR:F4JN26 | denotes | Mlh3 |
| T16 | 34-38 | PR:Q12083 | denotes | Mlh3 |
| T17 | 34-38 | PR:000010443 | denotes | Mlh3 |
| T18 | 34-38 | PR:Q9UHC1 | denotes | Mlh3 |
| T19 | 57-69 | P38446 | denotes | endonuclease |
| T20 | 57-69 | P13717 | denotes | endonuclease |
| T21 | 57-69 | P20314 | denotes | endonuclease |
| T22 | 99-117 | D045566 | denotes | Holliday junctions |
| T23 | 147-151 | PR:000023324 | denotes | MutL |
| T24 | 147-151 | PR:Q93T05 | denotes | MutL |
| T25 | 147-151 | PR:P23367 | denotes | MutL |
| T29 | 163-167 | CVCL_G669 | denotes | Mlh1 |
| T26 | 163-167 | PR:Q9ZRV4 | denotes | Mlh1 |
| T27 | 163-167 | PR:P38920 | denotes | Mlh1 |
| T28 | 163-167 | PR:Q54KD8 | denotes | Mlh1 |
| T30 | 163-167 | PR:Q9JK91 | denotes | Mlh1 |
| T31 | 163-167 | PR:P97679 | denotes | Mlh1 |
| T32 | 163-167 | PR:Q9P7W6 | denotes | Mlh1 |
| T33 | 163-167 | PR:000010442 | denotes | Mlh1 |
| T34 | 163-167 | PR:P40692 | denotes | Mlh1 |
| T35 | 172-176 | PR:F4JN26 | denotes | Mlh3 |
| T36 | 172-176 | PR:Q12083 | denotes | Mlh3 |
| T37 | 172-176 | PR:000010443 | denotes | Mlh3 |
| T38 | 172-176 | PR:Q9UHC1 | denotes | Mlh3 |
| T39 | 215-225 | SO:0000857 | denotes | homologous |
| T40 | 215-239 | GO:0035825 | denotes | homologous recombination |
| T41 | 265-269 | PR:F4JN26 | denotes | Mlh3 |
| T42 | 265-269 | PR:Q12083 | denotes | Mlh3 |
| T43 | 265-269 | PR:000010443 | denotes | Mlh3 |
| T44 | 265-269 | PR:Q9UHC1 | denotes | Mlh3 |
| T45 | 320-328 | P38446 | denotes | nuclease |
| T46 | 320-328 | P13717 | denotes | nuclease |
| T47 | 320-328 | P81204 | denotes | nuclease |
| T48 | 329-334 | SO:0100017 | denotes | motif |
| T49 | 329-334 | SO:0001067 | denotes | motif |
| T50 | 341-344 | CHEBI:64073 | denotes | EX4 |
| T51 | 378-386 | P38446 | denotes | nuclease |
| T52 | 378-386 | P13717 | denotes | nuclease |
| T53 | 378-386 | P81204 | denotes | nuclease |
| T54 | 378-395 | GO:0004518 | denotes | nuclease activity |
| T55 | 399-403 | PR:Q9ZRV4 | denotes | Mlh1 |
| T56 | 399-403 | PR:P38920 | denotes | Mlh1 |
| T57 | 399-403 | PR:Q54KD8 | denotes | Mlh1 |
| T59 | 399-403 | PR:Q9JK91 | denotes | Mlh1 |
| T60 | 399-403 | PR:P97679 | denotes | Mlh1 |
| T61 | 399-403 | PR:Q9P7W6 | denotes | Mlh1 |
| T62 | 399-403 | PR:000010442 | denotes | Mlh1 |
| T63 | 399-403 | PR:P40692 | denotes | Mlh1 |
| T58 | 399-403 | CVCL_G669 | denotes | Mlh1 |
| T64 | 404-408 | PR:F4JN26 | denotes | Mlh3 |
| T65 | 404-408 | PR:Q12083 | denotes | Mlh3 |
| T66 | 404-408 | PR:000010443 | denotes | Mlh3 |
| T67 | 404-408 | PR:Q9UHC1 | denotes | Mlh3 |
| T68 | 442-447 | UBERON:0004905 | denotes | joint |
| T69 | 442-447 | UBERON:0000982 | denotes | joint |
| T70 | 448-457 | CHEBI:25367 | denotes | molecules |
| T71 | 571-575 | PR:Q9ZRV4 | denotes | Mlh1 |
| T72 | 571-575 | PR:P38920 | denotes | Mlh1 |
| T73 | 571-575 | PR:Q54KD8 | denotes | Mlh1 |
| T75 | 571-575 | PR:Q9JK91 | denotes | Mlh1 |
| T76 | 571-575 | PR:P97679 | denotes | Mlh1 |
| T77 | 571-575 | PR:Q9P7W6 | denotes | Mlh1 |
| T78 | 571-575 | PR:000010442 | denotes | Mlh1 |
| T79 | 571-575 | PR:P40692 | denotes | Mlh1 |
| T74 | 571-575 | CVCL_G669 | denotes | Mlh1 |
| T80 | 576-580 | PR:F4JN26 | denotes | Mlh3 |
| T81 | 576-580 | PR:Q12083 | denotes | Mlh3 |
| T82 | 576-580 | PR:000010443 | denotes | Mlh3 |
| T83 | 576-580 | PR:Q9UHC1 | denotes | Mlh3 |
| T87 | 697-704 | PR:000000001 | denotes | protein |
| T88 | 697-704 | SO:0000104 | denotes | protein |
| T85 | 697-704 | CHEBI:36080 | denotes | protein |
| T86 | 697-704 | CHEBI:11122 | denotes | protein |
| T89 | 697-712 | GO:0032991 | denotes | protein complex |
| T91 | 736-741 | 214355 | denotes | yeast |
| T90 | 736-741 | D015003 | denotes | yeast |
| T92 | 742-746 | PR:Q9ZRV4 | denotes | Mlh1 |
| T93 | 742-746 | PR:P38920 | denotes | Mlh1 |
| T94 | 742-746 | PR:Q54KD8 | denotes | Mlh1 |
| T96 | 742-746 | PR:Q9JK91 | denotes | Mlh1 |
| T97 | 742-746 | PR:P97679 | denotes | Mlh1 |
| T98 | 742-746 | PR:Q9P7W6 | denotes | Mlh1 |
| T99 | 742-746 | PR:000010442 | denotes | Mlh1 |
| T100 | 742-746 | PR:P40692 | denotes | Mlh1 |
| T95 | 742-746 | CVCL_G669 | denotes | Mlh1 |
| T101 | 747-751 | PR:F4JN26 | denotes | Mlh3 |
| T102 | 747-751 | PR:Q12083 | denotes | Mlh3 |
| T103 | 747-751 | PR:000010443 | denotes | Mlh3 |
| T104 | 747-751 | PR:Q9UHC1 | denotes | Mlh3 |
| T105 | 828-832 | PR:000023324 | denotes | MutL |
| T106 | 828-832 | PR:Q93T05 | denotes | MutL |
| T107 | 828-832 | PR:P23367 | denotes | MutL |
| T108 | 839-847 | P38446 | denotes | nuclease |
| T109 | 839-847 | P13717 | denotes | nuclease |
| T110 | 839-847 | P81204 | denotes | nuclease |
| T111 | 859-865 | SO:0000985 | denotes | double |
| T112 | 859-878 | CHEBI:4705 | denotes | double-stranded DNA |
| T113 | 859-878 | D004247 | denotes | double-stranded DNA |
| T114 | 875-878 | GO:0005574 | denotes | DNA |
| T116 | 875-878 | SO:0000352 | denotes | DNA |
| T118 | 900-904 | PR:000023324 | denotes | MutL |
| T119 | 900-904 | PR:Q93T05 | denotes | MutL |
| T120 | 900-904 | PR:P23367 | denotes | MutL |
| T122 | 912-915 | CHEBI:16991 | denotes | DNA |
| T124 | 912-915 | D004247 | denotes | DNA |
| T123 | 912-915 | SO:0000352 | denotes | DNA |
| T121 | 912-915 | GO:0005574 | denotes | DNA |
| T125 | 971-989 | D045566 | denotes | Holliday junctions |
| T126 | 1013-1018 | D006801 | denotes | human |
| T130 | 1019-1023 | CVCL_G669 | denotes | MLH1 |
| T127 | 1019-1023 | PR:Q9ZRV4 | denotes | MLH1 |
| T128 | 1019-1023 | PR:P38920 | denotes | MLH1 |
| T129 | 1019-1023 | PR:Q54KD8 | denotes | MLH1 |
| T131 | 1019-1023 | PR:Q9JK91 | denotes | MLH1 |
| T132 | 1019-1023 | PR:P97679 | denotes | MLH1 |
| T133 | 1019-1023 | PR:Q9P7W6 | denotes | MLH1 |
| T134 | 1019-1023 | PR:000010442 | denotes | MLH1 |
| T135 | 1019-1023 | PR:P40692 | denotes | MLH1 |
| T136 | 1024-1028 | PR:F4JN26 | denotes | MLH3 |
| T137 | 1024-1028 | PR:Q12083 | denotes | MLH3 |
| T138 | 1024-1028 | PR:000010443 | denotes | MLH3 |
| T139 | 1024-1028 | PR:Q9UHC1 | denotes | MLH3 |
| T140 | 1064-1071 | SO:0100018 | denotes | binding |
| T141 | 1064-1071 | SO:0001091 | denotes | binding |
| T142 | 1064-1071 | GO:0005488 | denotes | binding |
| T143 | 1075-1093 | D045566 | denotes | Holliday junctions |
| T144 | 1099-1108 | SO:0000856 | denotes | conserved |
| T145 | 1132-1136 | PR:000023324 | denotes | MutL |
| T146 | 1132-1136 | PR:Q93T05 | denotes | MutL |
| T147 | 1132-1136 | PR:P23367 | denotes | MutL |
| T149 | 1158-1161 | CHEBI:16991 | denotes | DNA |
| T151 | 1158-1161 | D004247 | denotes | DNA |
| T150 | 1158-1161 | SO:0000352 | denotes | DNA |
| T148 | 1158-1161 | GO:0005574 | denotes | DNA |
| T152 | 1224-1228 | PR:000023324 | denotes | MutL |
| T153 | 1224-1228 | PR:Q93T05 | denotes | MutL |
| T154 | 1224-1228 | PR:P23367 | denotes | MutL |
| T155 | 1229-1238 | SO:0000853 | denotes | homologue |
| T156 | 1240-1244 | PR:000023324 | denotes | MutL |
| T157 | 1240-1244 | PR:Q93T05 | denotes | MutL |
| T158 | 1240-1244 | PR:P23367 | denotes | MutL |
| T159 | 1312-1316 | PR:000023324 | denotes | MutL |
| T160 | 1312-1316 | PR:Q93T05 | denotes | MutL |
| T161 | 1312-1316 | PR:P23367 | denotes | MutL |
| T165 | 1317-1324 | PR:000000001 | denotes | protein |
| T163 | 1317-1324 | CHEBI:36080 | denotes | protein |
| T164 | 1317-1324 | CHEBI:11122 | denotes | protein |
| T166 | 1317-1324 | SO:0000104 | denotes | protein |
| T162 | 1317-1324 | GO:0003675 | denotes | protein |
| T167 | 1370-1387 | D045566 | denotes | Holliday junction |
| T169 | 1379-1387 | SO:0000699 | denotes | junction |
| T168 | 1379-1387 | UBERON:0007651 | denotes | junction |
| T170 | 1414-1418 | PR:Q9ZRV4 | denotes | Mlh1 |
| T171 | 1414-1418 | PR:P38920 | denotes | Mlh1 |
| T172 | 1414-1418 | PR:Q54KD8 | denotes | Mlh1 |
| T174 | 1414-1418 | PR:Q9JK91 | denotes | Mlh1 |
| T175 | 1414-1418 | PR:P97679 | denotes | Mlh1 |
| T176 | 1414-1418 | PR:Q9P7W6 | denotes | Mlh1 |
| T177 | 1414-1418 | PR:000010442 | denotes | Mlh1 |
| T178 | 1414-1418 | PR:P40692 | denotes | Mlh1 |
| T173 | 1414-1418 | CVCL_G669 | denotes | Mlh1 |
| T179 | 1419-1423 | PR:F4JN26 | denotes | Mlh3 |
| T180 | 1419-1423 | PR:Q12083 | denotes | Mlh3 |
| T181 | 1419-1423 | PR:000010443 | denotes | Mlh3 |
| T182 | 1419-1423 | PR:Q9UHC1 | denotes | Mlh3 |
| T183 | 1459-1476 | D045566 | denotes | Holliday junction |
| T185 | 1468-1476 | SO:0000699 | denotes | junction |
| T184 | 1468-1476 | UBERON:0007651 | denotes | junction |
| T186 | 1521-1525 | PR:000023324 | denotes | MutL |
| T187 | 1521-1525 | PR:Q93T05 | denotes | MutL |
| T188 | 1521-1525 | PR:P23367 | denotes | MutL |
| T189 | 1558-1563 | UBERON:0004905 | denotes | joint |
| T190 | 1558-1563 | UBERON:0000982 | denotes | joint |
| T191 | 1564-1573 | CHEBI:25367 | denotes | molecules |
| T192 | 1600-1607 | GO:0051321 | denotes | meiosis |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-118 | Sentence | denotes | The Saccharomyces cerevisiae Mlh1-Mlh3 heterodimer is an endonuclease that preferentially binds to Holliday junctions. |
| T2 | 119-240 | Sentence | denotes | MutLγ, a heterodimer of the MutL homologues Mlh1 and Mlh3, plays a critical role during meiotic homologous recombination. |
| T3 | 241-503 | Sentence | denotes | The meiotic function of Mlh3 is fully dependent on the integrity of a putative nuclease motif DQHAX2EX4E, inferring that the anticipated nuclease activity of Mlh1-Mlh3 is involved in the processing of joint molecules to generate crossover recombination products. |
| T4 | 504-713 | Sentence | denotes | Although a vast body of genetic and cell biological data regarding Mlh1-Mlh3 is available, mechanistic insights into its function have been lacking due to the unavailability of the recombinant protein complex. |
| T5 | 714-802 | Sentence | denotes | Here we expressed the yeast Mlh1-Mlh3 heterodimer and purified it into near homogeneity. |
| T6 | 803-879 | Sentence | denotes | We show that recombinant MutLγ is a nuclease that nicks double-stranded DNA. |
| T7 | 880-990 | Sentence | denotes | We demonstrate that MutLγ binds DNA with a high affinity and shows a marked preference for Holliday junctions. |
| T8 | 991-1148 | Sentence | denotes | We also expressed the human MLH1-MLH3 complex and show that preferential binding to Holliday junctions is a conserved capacity of eukaryotic MutLγ complexes. |
| T9 | 1149-1239 | Sentence | denotes | Specific DNA recognition has never been observed with any other eukaryotic MutL homologue. |
| T10 | 1240-1332 | Sentence | denotes | MutLγ thus represents a new paradigm for the function of the eukaryotic MutL protein family. |
| T11 | 1333-1482 | Sentence | denotes | We provide insights into the mode of Holliday junction recognition and show that Mlh1-Mlh3 prefers to bind the open unstacked Holliday junction form. |
| T12 | 1483-1608 | Sentence | denotes | This further supports the model where MutLγ is part of a complex acting on joint molecules to generate crossovers in meiosis. |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 4-28 | OrganismTaxon | denotes | Saccharomyces cerevisiae | NCBItxid:4932 |
| T2 | 1013-1018 | OrganismTaxon | denotes | human | NCBItxid:9606 |
GlyCosmos6-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 29-33 | http://purl.obolibrary.org/obo/CLO_0007748 | denotes | Mlh1 |
| T2 | 163-167 | http://purl.obolibrary.org/obo/CLO_0007748 | denotes | Mlh1 |
| T3 | 387-395 | http://purl.obolibrary.org/obo/CLO_0001658 | denotes | activity |
| T4 | 399-403 | http://purl.obolibrary.org/obo/CLO_0007748 | denotes | Mlh1 |
| T5 | 540-544 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
| T6 | 571-575 | http://purl.obolibrary.org/obo/CLO_0007748 | denotes | Mlh1 |
| T7 | 697-712 | http://purl.obolibrary.org/obo/GO_0043234 | denotes | protein complex |
| T8 | 742-746 | http://purl.obolibrary.org/obo/CLO_0007748 | denotes | Mlh1 |
| T9 | 1019-1023 | http://purl.obolibrary.org/obo/CLO_0007748 | denotes | MLH1 |
| T10 | 1414-1418 | http://purl.obolibrary.org/obo/CLO_0007748 | denotes | Mlh1 |
UseCases_ArguminSci_Discourse
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-118 | DRI_Approach | denotes | The Saccharomyces cerevisiae Mlh1-Mlh3 heterodimer is an endonuclease that preferentially binds to Holliday junctions. |
| T2 | 119-240 | DRI_Challenge | denotes | MutLγ, a heterodimer of the MutL homologues Mlh1 and Mlh3, plays a critical role during meiotic homologous recombination. |
| T3 | 241-503 | DRI_Challenge | denotes | The meiotic function of Mlh3 is fully dependent on the integrity of a putative nuclease motif DQHAX2EX4E, inferring that the anticipated nuclease activity of Mlh1-Mlh3 is involved in the processing of joint molecules to generate crossover recombination products. |
| T4 | 504-713 | DRI_Background | denotes | Although a vast body of genetic and cell biological data regarding Mlh1-Mlh3 is available, mechanistic insights into its function have been lacking due to the unavailability of the recombinant protein complex. |
| T5 | 714-802 | DRI_Approach | denotes | Here we expressed the yeast Mlh1-Mlh3 heterodimer and purified it into near homogeneity. |
| T6 | 803-879 | DRI_Approach | denotes | We show that recombinant MutLγ is a nuclease that nicks double-stranded DNA. |
| T7 | 880-990 | DRI_Approach | denotes | We demonstrate that MutLγ binds DNA with a high affinity and shows a marked preference for Holliday junctions. |
| T8 | 991-1148 | DRI_Outcome | denotes | We also expressed the human MLH1-MLH3 complex and show that preferential binding to Holliday junctions is a conserved capacity of eukaryotic MutLγ complexes. |
| T9 | 1149-1239 | DRI_Outcome | denotes | Specific DNA recognition has never been observed with any other eukaryotic MutL homologue. |
| T10 | 1240-1332 | DRI_Approach | denotes | MutLγ thus represents a new paradigm for the function of the eukaryotic MutL protein family. |
| T11 | 1333-1482 | DRI_Outcome | denotes | We provide insights into the mode of Holliday junction recognition and show that Mlh1-Mlh3 prefers to bind the open unstacked Holliday junction form. |
| T12 | 1483-1608 | DRI_Approach | denotes | This further supports the model where MutLγ is part of a complex acting on joint molecules to generate crossovers in meiosis. |