> top > docs > PubMed:24443562 > annotations

PubMed:24443562 JSONTXT

Annnotations TAB JSON ListView MergeView

PubMed_ArguminSci

Id Subject Object Predicate Lexical cue
T6 880-990 DRI_Approach denotes We demonstrate that MutLγ binds DNA with a high affinity and shows a marked preference for Holliday junctions.
T7 991-1148 DRI_Outcome denotes We also expressed the human MLH1-MLH3 complex and show that preferential binding to Holliday junctions is a conserved capacity of eukaryotic MutLγ complexes.
T8 1149-1239 DRI_Outcome denotes Specific DNA recognition has never been observed with any other eukaryotic MutL homologue.
T9 1240-1332 DRI_Approach denotes MutLγ thus represents a new paradigm for the function of the eukaryotic MutL protein family.
T10 1333-1482 DRI_Outcome denotes We provide insights into the mode of Holliday junction recognition and show that Mlh1-Mlh3 prefers to bind the open unstacked Holliday junction form.
T11 1483-1608 DRI_Approach denotes This further supports the model where MutLγ is part of a complex acting on joint molecules to generate crossovers in meiosis.
T1 119-240 DRI_Challenge denotes MutLγ, a heterodimer of the MutL homologues Mlh1 and Mlh3, plays a critical role during meiotic homologous recombination.
T2 241-503 DRI_Challenge denotes The meiotic function of Mlh3 is fully dependent on the integrity of a putative nuclease motif DQHAX2EX4E, inferring that the anticipated nuclease activity of Mlh1-Mlh3 is involved in the processing of joint molecules to generate crossover recombination products.
T3 504-713 DRI_Background denotes Although a vast body of genetic and cell biological data regarding Mlh1-Mlh3 is available, mechanistic insights into its function have been lacking due to the unavailability of the recombinant protein complex.
T4 714-802 DRI_Approach denotes Here we expressed the yeast Mlh1-Mlh3 heterodimer and purified it into near homogeneity.
T5 803-879 DRI_Approach denotes We show that recombinant MutLγ is a nuclease that nicks double-stranded DNA.

c_corpus

Id Subject Object Predicate Lexical cue
T2 4-28 350141 denotes Saccharomyces cerevisiae
T5 4-28 9511 denotes Saccharomyces cerevisiae
T3 4-28 D012441 denotes Saccharomyces cerevisiae
T4 4-28 4932 denotes Saccharomyces cerevisiae
T9 29-33 CVCL_G669 denotes Mlh1
T6 29-33 PR:Q9ZRV4 denotes Mlh1
T7 29-33 PR:P38920 denotes Mlh1
T8 29-33 PR:Q54KD8 denotes Mlh1
T10 29-33 PR:Q9JK91 denotes Mlh1
T11 29-33 PR:P97679 denotes Mlh1
T12 29-33 PR:Q9P7W6 denotes Mlh1
T13 29-33 PR:000010442 denotes Mlh1
T14 29-33 PR:P40692 denotes Mlh1
T15 34-38 PR:F4JN26 denotes Mlh3
T16 34-38 PR:Q12083 denotes Mlh3
T17 34-38 PR:000010443 denotes Mlh3
T18 34-38 PR:Q9UHC1 denotes Mlh3
T19 57-69 P38446 denotes endonuclease
T20 57-69 P13717 denotes endonuclease
T21 57-69 P20314 denotes endonuclease
T22 99-117 D045566 denotes Holliday junctions
T23 147-151 PR:000023324 denotes MutL
T24 147-151 PR:Q93T05 denotes MutL
T25 147-151 PR:P23367 denotes MutL
T29 163-167 CVCL_G669 denotes Mlh1
T26 163-167 PR:Q9ZRV4 denotes Mlh1
T27 163-167 PR:P38920 denotes Mlh1
T28 163-167 PR:Q54KD8 denotes Mlh1
T30 163-167 PR:Q9JK91 denotes Mlh1
T31 163-167 PR:P97679 denotes Mlh1
T32 163-167 PR:Q9P7W6 denotes Mlh1
T33 163-167 PR:000010442 denotes Mlh1
T34 163-167 PR:P40692 denotes Mlh1
T35 172-176 PR:F4JN26 denotes Mlh3
T36 172-176 PR:Q12083 denotes Mlh3
T37 172-176 PR:000010443 denotes Mlh3
T38 172-176 PR:Q9UHC1 denotes Mlh3
T39 215-225 SO:0000857 denotes homologous
T40 215-239 GO:0035825 denotes homologous recombination
T41 265-269 PR:F4JN26 denotes Mlh3
T42 265-269 PR:Q12083 denotes Mlh3
T43 265-269 PR:000010443 denotes Mlh3
T44 265-269 PR:Q9UHC1 denotes Mlh3
T45 320-328 P38446 denotes nuclease
T46 320-328 P13717 denotes nuclease
T47 320-328 P81204 denotes nuclease
T48 329-334 SO:0100017 denotes motif
T49 329-334 SO:0001067 denotes motif
T50 341-344 CHEBI:64073 denotes EX4
T51 378-386 P38446 denotes nuclease
T52 378-386 P13717 denotes nuclease
T53 378-386 P81204 denotes nuclease
T54 378-395 GO:0004518 denotes nuclease activity
T55 399-403 PR:Q9ZRV4 denotes Mlh1
T56 399-403 PR:P38920 denotes Mlh1
T57 399-403 PR:Q54KD8 denotes Mlh1
T59 399-403 PR:Q9JK91 denotes Mlh1
T60 399-403 PR:P97679 denotes Mlh1
T61 399-403 PR:Q9P7W6 denotes Mlh1
T62 399-403 PR:000010442 denotes Mlh1
T63 399-403 PR:P40692 denotes Mlh1
T58 399-403 CVCL_G669 denotes Mlh1
T64 404-408 PR:F4JN26 denotes Mlh3
T65 404-408 PR:Q12083 denotes Mlh3
T66 404-408 PR:000010443 denotes Mlh3
T67 404-408 PR:Q9UHC1 denotes Mlh3
T68 442-447 UBERON:0004905 denotes joint
T69 442-447 UBERON:0000982 denotes joint
T70 448-457 CHEBI:25367 denotes molecules
T71 571-575 PR:Q9ZRV4 denotes Mlh1
T72 571-575 PR:P38920 denotes Mlh1
T73 571-575 PR:Q54KD8 denotes Mlh1
T75 571-575 PR:Q9JK91 denotes Mlh1
T76 571-575 PR:P97679 denotes Mlh1
T77 571-575 PR:Q9P7W6 denotes Mlh1
T78 571-575 PR:000010442 denotes Mlh1
T79 571-575 PR:P40692 denotes Mlh1
T74 571-575 CVCL_G669 denotes Mlh1
T80 576-580 PR:F4JN26 denotes Mlh3
T81 576-580 PR:Q12083 denotes Mlh3
T82 576-580 PR:000010443 denotes Mlh3
T83 576-580 PR:Q9UHC1 denotes Mlh3
T87 697-704 PR:000000001 denotes protein
T88 697-704 SO:0000104 denotes protein
T85 697-704 CHEBI:36080 denotes protein
T86 697-704 CHEBI:11122 denotes protein
T89 697-712 GO:0032991 denotes protein complex
T91 736-741 214355 denotes yeast
T90 736-741 D015003 denotes yeast
T92 742-746 PR:Q9ZRV4 denotes Mlh1
T93 742-746 PR:P38920 denotes Mlh1
T94 742-746 PR:Q54KD8 denotes Mlh1
T96 742-746 PR:Q9JK91 denotes Mlh1
T97 742-746 PR:P97679 denotes Mlh1
T98 742-746 PR:Q9P7W6 denotes Mlh1
T99 742-746 PR:000010442 denotes Mlh1
T100 742-746 PR:P40692 denotes Mlh1
T95 742-746 CVCL_G669 denotes Mlh1
T101 747-751 PR:F4JN26 denotes Mlh3
T102 747-751 PR:Q12083 denotes Mlh3
T103 747-751 PR:000010443 denotes Mlh3
T104 747-751 PR:Q9UHC1 denotes Mlh3
T105 828-832 PR:000023324 denotes MutL
T106 828-832 PR:Q93T05 denotes MutL
T107 828-832 PR:P23367 denotes MutL
T108 839-847 P38446 denotes nuclease
T109 839-847 P13717 denotes nuclease
T110 839-847 P81204 denotes nuclease
T111 859-865 SO:0000985 denotes double
T112 859-878 CHEBI:4705 denotes double-stranded DNA
T113 859-878 D004247 denotes double-stranded DNA
T114 875-878 GO:0005574 denotes DNA
T116 875-878 SO:0000352 denotes DNA
T118 900-904 PR:000023324 denotes MutL
T119 900-904 PR:Q93T05 denotes MutL
T120 900-904 PR:P23367 denotes MutL
T122 912-915 CHEBI:16991 denotes DNA
T124 912-915 D004247 denotes DNA
T123 912-915 SO:0000352 denotes DNA
T121 912-915 GO:0005574 denotes DNA
T125 971-989 D045566 denotes Holliday junctions
T126 1013-1018 D006801 denotes human
T130 1019-1023 CVCL_G669 denotes MLH1
T127 1019-1023 PR:Q9ZRV4 denotes MLH1
T128 1019-1023 PR:P38920 denotes MLH1
T129 1019-1023 PR:Q54KD8 denotes MLH1
T131 1019-1023 PR:Q9JK91 denotes MLH1
T132 1019-1023 PR:P97679 denotes MLH1
T133 1019-1023 PR:Q9P7W6 denotes MLH1
T134 1019-1023 PR:000010442 denotes MLH1
T135 1019-1023 PR:P40692 denotes MLH1
T136 1024-1028 PR:F4JN26 denotes MLH3
T137 1024-1028 PR:Q12083 denotes MLH3
T138 1024-1028 PR:000010443 denotes MLH3
T139 1024-1028 PR:Q9UHC1 denotes MLH3
T140 1064-1071 SO:0100018 denotes binding
T141 1064-1071 SO:0001091 denotes binding
T142 1064-1071 GO:0005488 denotes binding
T143 1075-1093 D045566 denotes Holliday junctions
T144 1099-1108 SO:0000856 denotes conserved
T145 1132-1136 PR:000023324 denotes MutL
T146 1132-1136 PR:Q93T05 denotes MutL
T147 1132-1136 PR:P23367 denotes MutL
T149 1158-1161 CHEBI:16991 denotes DNA
T151 1158-1161 D004247 denotes DNA
T150 1158-1161 SO:0000352 denotes DNA
T148 1158-1161 GO:0005574 denotes DNA
T152 1224-1228 PR:000023324 denotes MutL
T153 1224-1228 PR:Q93T05 denotes MutL
T154 1224-1228 PR:P23367 denotes MutL
T155 1229-1238 SO:0000853 denotes homologue
T156 1240-1244 PR:000023324 denotes MutL
T157 1240-1244 PR:Q93T05 denotes MutL
T158 1240-1244 PR:P23367 denotes MutL
T159 1312-1316 PR:000023324 denotes MutL
T160 1312-1316 PR:Q93T05 denotes MutL
T161 1312-1316 PR:P23367 denotes MutL
T165 1317-1324 PR:000000001 denotes protein
T163 1317-1324 CHEBI:36080 denotes protein
T164 1317-1324 CHEBI:11122 denotes protein
T166 1317-1324 SO:0000104 denotes protein
T162 1317-1324 GO:0003675 denotes protein
T167 1370-1387 D045566 denotes Holliday junction
T169 1379-1387 SO:0000699 denotes junction
T168 1379-1387 UBERON:0007651 denotes junction
T170 1414-1418 PR:Q9ZRV4 denotes Mlh1
T171 1414-1418 PR:P38920 denotes Mlh1
T172 1414-1418 PR:Q54KD8 denotes Mlh1
T174 1414-1418 PR:Q9JK91 denotes Mlh1
T175 1414-1418 PR:P97679 denotes Mlh1
T176 1414-1418 PR:Q9P7W6 denotes Mlh1
T177 1414-1418 PR:000010442 denotes Mlh1
T178 1414-1418 PR:P40692 denotes Mlh1
T173 1414-1418 CVCL_G669 denotes Mlh1
T179 1419-1423 PR:F4JN26 denotes Mlh3
T180 1419-1423 PR:Q12083 denotes Mlh3
T181 1419-1423 PR:000010443 denotes Mlh3
T182 1419-1423 PR:Q9UHC1 denotes Mlh3
T183 1459-1476 D045566 denotes Holliday junction
T185 1468-1476 SO:0000699 denotes junction
T184 1468-1476 UBERON:0007651 denotes junction
T186 1521-1525 PR:000023324 denotes MutL
T187 1521-1525 PR:Q93T05 denotes MutL
T188 1521-1525 PR:P23367 denotes MutL
T189 1558-1563 UBERON:0004905 denotes joint
T190 1558-1563 UBERON:0000982 denotes joint
T191 1564-1573 CHEBI:25367 denotes molecules
T192 1600-1607 GO:0051321 denotes meiosis

sentences

Id Subject Object Predicate Lexical cue
T1 0-118 Sentence denotes The Saccharomyces cerevisiae Mlh1-Mlh3 heterodimer is an endonuclease that preferentially binds to Holliday junctions.
T2 119-240 Sentence denotes MutLγ, a heterodimer of the MutL homologues Mlh1 and Mlh3, plays a critical role during meiotic homologous recombination.
T3 241-503 Sentence denotes The meiotic function of Mlh3 is fully dependent on the integrity of a putative nuclease motif DQHAX2EX4E, inferring that the anticipated nuclease activity of Mlh1-Mlh3 is involved in the processing of joint molecules to generate crossover recombination products.
T4 504-713 Sentence denotes Although a vast body of genetic and cell biological data regarding Mlh1-Mlh3 is available, mechanistic insights into its function have been lacking due to the unavailability of the recombinant protein complex.
T5 714-802 Sentence denotes Here we expressed the yeast Mlh1-Mlh3 heterodimer and purified it into near homogeneity.
T6 803-879 Sentence denotes We show that recombinant MutLγ is a nuclease that nicks double-stranded DNA.
T7 880-990 Sentence denotes We demonstrate that MutLγ binds DNA with a high affinity and shows a marked preference for Holliday junctions.
T8 991-1148 Sentence denotes We also expressed the human MLH1-MLH3 complex and show that preferential binding to Holliday junctions is a conserved capacity of eukaryotic MutLγ complexes.
T9 1149-1239 Sentence denotes Specific DNA recognition has never been observed with any other eukaryotic MutL homologue.
T10 1240-1332 Sentence denotes MutLγ thus represents a new paradigm for the function of the eukaryotic MutL protein family.
T11 1333-1482 Sentence denotes We provide insights into the mode of Holliday junction recognition and show that Mlh1-Mlh3 prefers to bind the open unstacked Holliday junction form.
T12 1483-1608 Sentence denotes This further supports the model where MutLγ is part of a complex acting on joint molecules to generate crossovers in meiosis.

UseCases_ArguminSci_Discourse

Id Subject Object Predicate Lexical cue
T1 0-118 DRI_Approach denotes The Saccharomyces cerevisiae Mlh1-Mlh3 heterodimer is an endonuclease that preferentially binds to Holliday junctions.
T2 119-240 DRI_Challenge denotes MutLγ, a heterodimer of the MutL homologues Mlh1 and Mlh3, plays a critical role during meiotic homologous recombination.
T3 241-503 DRI_Challenge denotes The meiotic function of Mlh3 is fully dependent on the integrity of a putative nuclease motif DQHAX2EX4E, inferring that the anticipated nuclease activity of Mlh1-Mlh3 is involved in the processing of joint molecules to generate crossover recombination products.
T4 504-713 DRI_Background denotes Although a vast body of genetic and cell biological data regarding Mlh1-Mlh3 is available, mechanistic insights into its function have been lacking due to the unavailability of the recombinant protein complex.
T5 714-802 DRI_Approach denotes Here we expressed the yeast Mlh1-Mlh3 heterodimer and purified it into near homogeneity.
T6 803-879 DRI_Approach denotes We show that recombinant MutLγ is a nuclease that nicks double-stranded DNA.
T7 880-990 DRI_Approach denotes We demonstrate that MutLγ binds DNA with a high affinity and shows a marked preference for Holliday junctions.
T8 991-1148 DRI_Outcome denotes We also expressed the human MLH1-MLH3 complex and show that preferential binding to Holliday junctions is a conserved capacity of eukaryotic MutLγ complexes.
T9 1149-1239 DRI_Outcome denotes Specific DNA recognition has never been observed with any other eukaryotic MutL homologue.
T10 1240-1332 DRI_Approach denotes MutLγ thus represents a new paradigm for the function of the eukaryotic MutL protein family.
T11 1333-1482 DRI_Outcome denotes We provide insights into the mode of Holliday junction recognition and show that Mlh1-Mlh3 prefers to bind the open unstacked Holliday junction form.
T12 1483-1608 DRI_Approach denotes This further supports the model where MutLγ is part of a complex acting on joint molecules to generate crossovers in meiosis.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 4-28 OrganismTaxon denotes Saccharomyces cerevisiae 4932
T2 1013-1018 OrganismTaxon denotes human 9606

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 108-117 Body_part denotes junctions http://purl.obolibrary.org/obo/UBERON_0007651
T2 442-447 Body_part denotes joint http://purl.obolibrary.org/obo/UBERON_0000982|http://purl.obolibrary.org/obo/UBERON_0004905
T4 980-989 Body_part denotes junctions http://purl.obolibrary.org/obo/UBERON_0007651
T5 1084-1093 Body_part denotes junctions http://purl.obolibrary.org/obo/UBERON_0007651
T6 1379-1387 Body_part denotes junction http://purl.obolibrary.org/obo/UBERON_0007651
T7 1468-1476 Body_part denotes junction http://purl.obolibrary.org/obo/UBERON_0007651
T8 1558-1563 Body_part denotes joint http://purl.obolibrary.org/obo/UBERON_0000982|http://purl.obolibrary.org/obo/UBERON_0004905