PubMed:24443562
Annnotations
PubMed_ArguminSci
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6 | 880-990 | DRI_Approach | denotes | We demonstrate that MutLγ binds DNA with a high affinity and shows a marked preference for Holliday junctions. |
T7 | 991-1148 | DRI_Outcome | denotes | We also expressed the human MLH1-MLH3 complex and show that preferential binding to Holliday junctions is a conserved capacity of eukaryotic MutLγ complexes. |
T8 | 1149-1239 | DRI_Outcome | denotes | Specific DNA recognition has never been observed with any other eukaryotic MutL homologue. |
T9 | 1240-1332 | DRI_Approach | denotes | MutLγ thus represents a new paradigm for the function of the eukaryotic MutL protein family. |
T10 | 1333-1482 | DRI_Outcome | denotes | We provide insights into the mode of Holliday junction recognition and show that Mlh1-Mlh3 prefers to bind the open unstacked Holliday junction form. |
T11 | 1483-1608 | DRI_Approach | denotes | This further supports the model where MutLγ is part of a complex acting on joint molecules to generate crossovers in meiosis. |
T1 | 119-240 | DRI_Challenge | denotes | MutLγ, a heterodimer of the MutL homologues Mlh1 and Mlh3, plays a critical role during meiotic homologous recombination. |
T2 | 241-503 | DRI_Challenge | denotes | The meiotic function of Mlh3 is fully dependent on the integrity of a putative nuclease motif DQHAX2EX4E, inferring that the anticipated nuclease activity of Mlh1-Mlh3 is involved in the processing of joint molecules to generate crossover recombination products. |
T3 | 504-713 | DRI_Background | denotes | Although a vast body of genetic and cell biological data regarding Mlh1-Mlh3 is available, mechanistic insights into its function have been lacking due to the unavailability of the recombinant protein complex. |
T4 | 714-802 | DRI_Approach | denotes | Here we expressed the yeast Mlh1-Mlh3 heterodimer and purified it into near homogeneity. |
T5 | 803-879 | DRI_Approach | denotes | We show that recombinant MutLγ is a nuclease that nicks double-stranded DNA. |
c_corpus
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T2 | 4-28 | 350141 | denotes | Saccharomyces cerevisiae |
T5 | 4-28 | 9511 | denotes | Saccharomyces cerevisiae |
T3 | 4-28 | D012441 | denotes | Saccharomyces cerevisiae |
T4 | 4-28 | 4932 | denotes | Saccharomyces cerevisiae |
T9 | 29-33 | CVCL_G669 | denotes | Mlh1 |
T6 | 29-33 | PR:Q9ZRV4 | denotes | Mlh1 |
T7 | 29-33 | PR:P38920 | denotes | Mlh1 |
T8 | 29-33 | PR:Q54KD8 | denotes | Mlh1 |
T10 | 29-33 | PR:Q9JK91 | denotes | Mlh1 |
T11 | 29-33 | PR:P97679 | denotes | Mlh1 |
T12 | 29-33 | PR:Q9P7W6 | denotes | Mlh1 |
T13 | 29-33 | PR:000010442 | denotes | Mlh1 |
T14 | 29-33 | PR:P40692 | denotes | Mlh1 |
T15 | 34-38 | PR:F4JN26 | denotes | Mlh3 |
T16 | 34-38 | PR:Q12083 | denotes | Mlh3 |
T17 | 34-38 | PR:000010443 | denotes | Mlh3 |
T18 | 34-38 | PR:Q9UHC1 | denotes | Mlh3 |
T19 | 57-69 | P38446 | denotes | endonuclease |
T20 | 57-69 | P13717 | denotes | endonuclease |
T21 | 57-69 | P20314 | denotes | endonuclease |
T22 | 99-117 | D045566 | denotes | Holliday junctions |
T23 | 147-151 | PR:000023324 | denotes | MutL |
T24 | 147-151 | PR:Q93T05 | denotes | MutL |
T25 | 147-151 | PR:P23367 | denotes | MutL |
T29 | 163-167 | CVCL_G669 | denotes | Mlh1 |
T26 | 163-167 | PR:Q9ZRV4 | denotes | Mlh1 |
T27 | 163-167 | PR:P38920 | denotes | Mlh1 |
T28 | 163-167 | PR:Q54KD8 | denotes | Mlh1 |
T30 | 163-167 | PR:Q9JK91 | denotes | Mlh1 |
T31 | 163-167 | PR:P97679 | denotes | Mlh1 |
T32 | 163-167 | PR:Q9P7W6 | denotes | Mlh1 |
T33 | 163-167 | PR:000010442 | denotes | Mlh1 |
T34 | 163-167 | PR:P40692 | denotes | Mlh1 |
T35 | 172-176 | PR:F4JN26 | denotes | Mlh3 |
T36 | 172-176 | PR:Q12083 | denotes | Mlh3 |
T37 | 172-176 | PR:000010443 | denotes | Mlh3 |
T38 | 172-176 | PR:Q9UHC1 | denotes | Mlh3 |
T39 | 215-225 | SO:0000857 | denotes | homologous |
T40 | 215-239 | GO:0035825 | denotes | homologous recombination |
T41 | 265-269 | PR:F4JN26 | denotes | Mlh3 |
T42 | 265-269 | PR:Q12083 | denotes | Mlh3 |
T43 | 265-269 | PR:000010443 | denotes | Mlh3 |
T44 | 265-269 | PR:Q9UHC1 | denotes | Mlh3 |
T45 | 320-328 | P38446 | denotes | nuclease |
T46 | 320-328 | P13717 | denotes | nuclease |
T47 | 320-328 | P81204 | denotes | nuclease |
T48 | 329-334 | SO:0100017 | denotes | motif |
T49 | 329-334 | SO:0001067 | denotes | motif |
T50 | 341-344 | CHEBI:64073 | denotes | EX4 |
T51 | 378-386 | P38446 | denotes | nuclease |
T52 | 378-386 | P13717 | denotes | nuclease |
T53 | 378-386 | P81204 | denotes | nuclease |
T54 | 378-395 | GO:0004518 | denotes | nuclease activity |
T55 | 399-403 | PR:Q9ZRV4 | denotes | Mlh1 |
T56 | 399-403 | PR:P38920 | denotes | Mlh1 |
T57 | 399-403 | PR:Q54KD8 | denotes | Mlh1 |
T59 | 399-403 | PR:Q9JK91 | denotes | Mlh1 |
T60 | 399-403 | PR:P97679 | denotes | Mlh1 |
T61 | 399-403 | PR:Q9P7W6 | denotes | Mlh1 |
T62 | 399-403 | PR:000010442 | denotes | Mlh1 |
T63 | 399-403 | PR:P40692 | denotes | Mlh1 |
T58 | 399-403 | CVCL_G669 | denotes | Mlh1 |
T64 | 404-408 | PR:F4JN26 | denotes | Mlh3 |
T65 | 404-408 | PR:Q12083 | denotes | Mlh3 |
T66 | 404-408 | PR:000010443 | denotes | Mlh3 |
T67 | 404-408 | PR:Q9UHC1 | denotes | Mlh3 |
T68 | 442-447 | UBERON:0004905 | denotes | joint |
T69 | 442-447 | UBERON:0000982 | denotes | joint |
T70 | 448-457 | CHEBI:25367 | denotes | molecules |
T71 | 571-575 | PR:Q9ZRV4 | denotes | Mlh1 |
T72 | 571-575 | PR:P38920 | denotes | Mlh1 |
T73 | 571-575 | PR:Q54KD8 | denotes | Mlh1 |
T75 | 571-575 | PR:Q9JK91 | denotes | Mlh1 |
T76 | 571-575 | PR:P97679 | denotes | Mlh1 |
T77 | 571-575 | PR:Q9P7W6 | denotes | Mlh1 |
T78 | 571-575 | PR:000010442 | denotes | Mlh1 |
T79 | 571-575 | PR:P40692 | denotes | Mlh1 |
T74 | 571-575 | CVCL_G669 | denotes | Mlh1 |
T80 | 576-580 | PR:F4JN26 | denotes | Mlh3 |
T81 | 576-580 | PR:Q12083 | denotes | Mlh3 |
T82 | 576-580 | PR:000010443 | denotes | Mlh3 |
T83 | 576-580 | PR:Q9UHC1 | denotes | Mlh3 |
T87 | 697-704 | PR:000000001 | denotes | protein |
T88 | 697-704 | SO:0000104 | denotes | protein |
T85 | 697-704 | CHEBI:36080 | denotes | protein |
T86 | 697-704 | CHEBI:11122 | denotes | protein |
T89 | 697-712 | GO:0032991 | denotes | protein complex |
T91 | 736-741 | 214355 | denotes | yeast |
T90 | 736-741 | D015003 | denotes | yeast |
T92 | 742-746 | PR:Q9ZRV4 | denotes | Mlh1 |
T93 | 742-746 | PR:P38920 | denotes | Mlh1 |
T94 | 742-746 | PR:Q54KD8 | denotes | Mlh1 |
T96 | 742-746 | PR:Q9JK91 | denotes | Mlh1 |
T97 | 742-746 | PR:P97679 | denotes | Mlh1 |
T98 | 742-746 | PR:Q9P7W6 | denotes | Mlh1 |
T99 | 742-746 | PR:000010442 | denotes | Mlh1 |
T100 | 742-746 | PR:P40692 | denotes | Mlh1 |
T95 | 742-746 | CVCL_G669 | denotes | Mlh1 |
T101 | 747-751 | PR:F4JN26 | denotes | Mlh3 |
T102 | 747-751 | PR:Q12083 | denotes | Mlh3 |
T103 | 747-751 | PR:000010443 | denotes | Mlh3 |
T104 | 747-751 | PR:Q9UHC1 | denotes | Mlh3 |
T105 | 828-832 | PR:000023324 | denotes | MutL |
T106 | 828-832 | PR:Q93T05 | denotes | MutL |
T107 | 828-832 | PR:P23367 | denotes | MutL |
T108 | 839-847 | P38446 | denotes | nuclease |
T109 | 839-847 | P13717 | denotes | nuclease |
T110 | 839-847 | P81204 | denotes | nuclease |
T111 | 859-865 | SO:0000985 | denotes | double |
T112 | 859-878 | CHEBI:4705 | denotes | double-stranded DNA |
T113 | 859-878 | D004247 | denotes | double-stranded DNA |
T114 | 875-878 | GO:0005574 | denotes | DNA |
T116 | 875-878 | SO:0000352 | denotes | DNA |
T118 | 900-904 | PR:000023324 | denotes | MutL |
T119 | 900-904 | PR:Q93T05 | denotes | MutL |
T120 | 900-904 | PR:P23367 | denotes | MutL |
T122 | 912-915 | CHEBI:16991 | denotes | DNA |
T124 | 912-915 | D004247 | denotes | DNA |
T123 | 912-915 | SO:0000352 | denotes | DNA |
T121 | 912-915 | GO:0005574 | denotes | DNA |
T125 | 971-989 | D045566 | denotes | Holliday junctions |
T126 | 1013-1018 | D006801 | denotes | human |
T130 | 1019-1023 | CVCL_G669 | denotes | MLH1 |
T127 | 1019-1023 | PR:Q9ZRV4 | denotes | MLH1 |
T128 | 1019-1023 | PR:P38920 | denotes | MLH1 |
T129 | 1019-1023 | PR:Q54KD8 | denotes | MLH1 |
T131 | 1019-1023 | PR:Q9JK91 | denotes | MLH1 |
T132 | 1019-1023 | PR:P97679 | denotes | MLH1 |
T133 | 1019-1023 | PR:Q9P7W6 | denotes | MLH1 |
T134 | 1019-1023 | PR:000010442 | denotes | MLH1 |
T135 | 1019-1023 | PR:P40692 | denotes | MLH1 |
T136 | 1024-1028 | PR:F4JN26 | denotes | MLH3 |
T137 | 1024-1028 | PR:Q12083 | denotes | MLH3 |
T138 | 1024-1028 | PR:000010443 | denotes | MLH3 |
T139 | 1024-1028 | PR:Q9UHC1 | denotes | MLH3 |
T140 | 1064-1071 | SO:0100018 | denotes | binding |
T141 | 1064-1071 | SO:0001091 | denotes | binding |
T142 | 1064-1071 | GO:0005488 | denotes | binding |
T143 | 1075-1093 | D045566 | denotes | Holliday junctions |
T144 | 1099-1108 | SO:0000856 | denotes | conserved |
T145 | 1132-1136 | PR:000023324 | denotes | MutL |
T146 | 1132-1136 | PR:Q93T05 | denotes | MutL |
T147 | 1132-1136 | PR:P23367 | denotes | MutL |
T149 | 1158-1161 | CHEBI:16991 | denotes | DNA |
T151 | 1158-1161 | D004247 | denotes | DNA |
T150 | 1158-1161 | SO:0000352 | denotes | DNA |
T148 | 1158-1161 | GO:0005574 | denotes | DNA |
T152 | 1224-1228 | PR:000023324 | denotes | MutL |
T153 | 1224-1228 | PR:Q93T05 | denotes | MutL |
T154 | 1224-1228 | PR:P23367 | denotes | MutL |
T155 | 1229-1238 | SO:0000853 | denotes | homologue |
T156 | 1240-1244 | PR:000023324 | denotes | MutL |
T157 | 1240-1244 | PR:Q93T05 | denotes | MutL |
T158 | 1240-1244 | PR:P23367 | denotes | MutL |
T159 | 1312-1316 | PR:000023324 | denotes | MutL |
T160 | 1312-1316 | PR:Q93T05 | denotes | MutL |
T161 | 1312-1316 | PR:P23367 | denotes | MutL |
T165 | 1317-1324 | PR:000000001 | denotes | protein |
T163 | 1317-1324 | CHEBI:36080 | denotes | protein |
T164 | 1317-1324 | CHEBI:11122 | denotes | protein |
T166 | 1317-1324 | SO:0000104 | denotes | protein |
T162 | 1317-1324 | GO:0003675 | denotes | protein |
T167 | 1370-1387 | D045566 | denotes | Holliday junction |
T169 | 1379-1387 | SO:0000699 | denotes | junction |
T168 | 1379-1387 | UBERON:0007651 | denotes | junction |
T170 | 1414-1418 | PR:Q9ZRV4 | denotes | Mlh1 |
T171 | 1414-1418 | PR:P38920 | denotes | Mlh1 |
T172 | 1414-1418 | PR:Q54KD8 | denotes | Mlh1 |
T174 | 1414-1418 | PR:Q9JK91 | denotes | Mlh1 |
T175 | 1414-1418 | PR:P97679 | denotes | Mlh1 |
T176 | 1414-1418 | PR:Q9P7W6 | denotes | Mlh1 |
T177 | 1414-1418 | PR:000010442 | denotes | Mlh1 |
T178 | 1414-1418 | PR:P40692 | denotes | Mlh1 |
T173 | 1414-1418 | CVCL_G669 | denotes | Mlh1 |
T179 | 1419-1423 | PR:F4JN26 | denotes | Mlh3 |
T180 | 1419-1423 | PR:Q12083 | denotes | Mlh3 |
T181 | 1419-1423 | PR:000010443 | denotes | Mlh3 |
T182 | 1419-1423 | PR:Q9UHC1 | denotes | Mlh3 |
T183 | 1459-1476 | D045566 | denotes | Holliday junction |
T185 | 1468-1476 | SO:0000699 | denotes | junction |
T184 | 1468-1476 | UBERON:0007651 | denotes | junction |
T186 | 1521-1525 | PR:000023324 | denotes | MutL |
T187 | 1521-1525 | PR:Q93T05 | denotes | MutL |
T188 | 1521-1525 | PR:P23367 | denotes | MutL |
T189 | 1558-1563 | UBERON:0004905 | denotes | joint |
T190 | 1558-1563 | UBERON:0000982 | denotes | joint |
T191 | 1564-1573 | CHEBI:25367 | denotes | molecules |
T192 | 1600-1607 | GO:0051321 | denotes | meiosis |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-118 | Sentence | denotes | The Saccharomyces cerevisiae Mlh1-Mlh3 heterodimer is an endonuclease that preferentially binds to Holliday junctions. |
T2 | 119-240 | Sentence | denotes | MutLγ, a heterodimer of the MutL homologues Mlh1 and Mlh3, plays a critical role during meiotic homologous recombination. |
T3 | 241-503 | Sentence | denotes | The meiotic function of Mlh3 is fully dependent on the integrity of a putative nuclease motif DQHAX2EX4E, inferring that the anticipated nuclease activity of Mlh1-Mlh3 is involved in the processing of joint molecules to generate crossover recombination products. |
T4 | 504-713 | Sentence | denotes | Although a vast body of genetic and cell biological data regarding Mlh1-Mlh3 is available, mechanistic insights into its function have been lacking due to the unavailability of the recombinant protein complex. |
T5 | 714-802 | Sentence | denotes | Here we expressed the yeast Mlh1-Mlh3 heterodimer and purified it into near homogeneity. |
T6 | 803-879 | Sentence | denotes | We show that recombinant MutLγ is a nuclease that nicks double-stranded DNA. |
T7 | 880-990 | Sentence | denotes | We demonstrate that MutLγ binds DNA with a high affinity and shows a marked preference for Holliday junctions. |
T8 | 991-1148 | Sentence | denotes | We also expressed the human MLH1-MLH3 complex and show that preferential binding to Holliday junctions is a conserved capacity of eukaryotic MutLγ complexes. |
T9 | 1149-1239 | Sentence | denotes | Specific DNA recognition has never been observed with any other eukaryotic MutL homologue. |
T10 | 1240-1332 | Sentence | denotes | MutLγ thus represents a new paradigm for the function of the eukaryotic MutL protein family. |
T11 | 1333-1482 | Sentence | denotes | We provide insights into the mode of Holliday junction recognition and show that Mlh1-Mlh3 prefers to bind the open unstacked Holliday junction form. |
T12 | 1483-1608 | Sentence | denotes | This further supports the model where MutLγ is part of a complex acting on joint molecules to generate crossovers in meiosis. |
UseCases_ArguminSci_Discourse
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-118 | DRI_Approach | denotes | The Saccharomyces cerevisiae Mlh1-Mlh3 heterodimer is an endonuclease that preferentially binds to Holliday junctions. |
T2 | 119-240 | DRI_Challenge | denotes | MutLγ, a heterodimer of the MutL homologues Mlh1 and Mlh3, plays a critical role during meiotic homologous recombination. |
T3 | 241-503 | DRI_Challenge | denotes | The meiotic function of Mlh3 is fully dependent on the integrity of a putative nuclease motif DQHAX2EX4E, inferring that the anticipated nuclease activity of Mlh1-Mlh3 is involved in the processing of joint molecules to generate crossover recombination products. |
T4 | 504-713 | DRI_Background | denotes | Although a vast body of genetic and cell biological data regarding Mlh1-Mlh3 is available, mechanistic insights into its function have been lacking due to the unavailability of the recombinant protein complex. |
T5 | 714-802 | DRI_Approach | denotes | Here we expressed the yeast Mlh1-Mlh3 heterodimer and purified it into near homogeneity. |
T6 | 803-879 | DRI_Approach | denotes | We show that recombinant MutLγ is a nuclease that nicks double-stranded DNA. |
T7 | 880-990 | DRI_Approach | denotes | We demonstrate that MutLγ binds DNA with a high affinity and shows a marked preference for Holliday junctions. |
T8 | 991-1148 | DRI_Outcome | denotes | We also expressed the human MLH1-MLH3 complex and show that preferential binding to Holliday junctions is a conserved capacity of eukaryotic MutLγ complexes. |
T9 | 1149-1239 | DRI_Outcome | denotes | Specific DNA recognition has never been observed with any other eukaryotic MutL homologue. |
T10 | 1240-1332 | DRI_Approach | denotes | MutLγ thus represents a new paradigm for the function of the eukaryotic MutL protein family. |
T11 | 1333-1482 | DRI_Outcome | denotes | We provide insights into the mode of Holliday junction recognition and show that Mlh1-Mlh3 prefers to bind the open unstacked Holliday junction form. |
T12 | 1483-1608 | DRI_Approach | denotes | This further supports the model where MutLγ is part of a complex acting on joint molecules to generate crossovers in meiosis. |
NCBITAXON
Id | Subject | Object | Predicate | Lexical cue | db_id |
---|---|---|---|---|---|
T1 | 4-28 | OrganismTaxon | denotes | Saccharomyces cerevisiae | 4932 |
T2 | 1013-1018 | OrganismTaxon | denotes | human | 9606 |
Anatomy-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T1 | 108-117 | Body_part | denotes | junctions | http://purl.obolibrary.org/obo/UBERON_0007651 |
T2 | 442-447 | Body_part | denotes | joint | http://purl.obolibrary.org/obo/UBERON_0000982|http://purl.obolibrary.org/obo/UBERON_0004905 |
T4 | 980-989 | Body_part | denotes | junctions | http://purl.obolibrary.org/obo/UBERON_0007651 |
T5 | 1084-1093 | Body_part | denotes | junctions | http://purl.obolibrary.org/obo/UBERON_0007651 |
T6 | 1379-1387 | Body_part | denotes | junction | http://purl.obolibrary.org/obo/UBERON_0007651 |
T7 | 1468-1476 | Body_part | denotes | junction | http://purl.obolibrary.org/obo/UBERON_0007651 |
T8 | 1558-1563 | Body_part | denotes | joint | http://purl.obolibrary.org/obo/UBERON_0000982|http://purl.obolibrary.org/obo/UBERON_0004905 |