PubMed:20053629 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/20053629","sourcedb":"PubMed","sourceid":"20053629","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/20053629","text":"The novel proapoptotic activity of nonnatural enantiomer of Lentiginosine.\nD-(-)-Lentiginosine [(-)-4], the nonnatural enantiomer of the iminosugar indolizidine alkaloid L-(+)-lentiginosine, acts as apoptosis inducer on tumor cells of different origin, in contrast to its natural enantiomer. Although D-(-)-4 exhibited a proapoptotic activity towards tumor cells at level lower than the chemotherapeutic agent, SN38, it was less proapoptotic towards normal cells and less cytotoxic. Apoptosis induced by D-(-)-4 was caspase-dependent, as shown by the increased expression and activity of caspase-3 and -8 in treated cells, and by inhibition following treatment with the pan caspase inhibitor, ZVAD-FMK. This study highlighted how a natural iminosugar alkaloid and its synthetic enantiomer, which were simply known for their inhibition against a fungal glucoamylase, could behave in a complete different way when tested towards cell growth and death of cells of different 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