PubMed:15671273 / 332-967 JSONTXT

{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T2","span":{"begin":0,"end":635},"obj":"METHODS"}],"text":"Rabbit corneal myofibroblasts were identified by immunodetection of alpha-smooth muscle (alpha-SM)-actin. MT1-MMP, MMP-2, MMP-9, and tissue inhibitor of matrix metalloproteinase (TIMP)-2 were detected by immunofluorescence. Cells were treated with 100 nM cPAF, with or without the PAF antagonist BN 50730 or the furin inhibitor nona-D-arg-NH(2). Gene-expression levels for furin, urokinase plasminogen activator, MMP-2, MMP-9, MT1-MMP, and TIMP-2 were determined by real-time PCR. Protein expression was assessed by Western blot. MMP-2 and -9 activity was determined by gelatin zymography. Active MT1-MMP levels were measured by ELISA."}

{"project":"c_corpus","denotations":[{"id":"T113","span":{"begin":0,"end":6},"obj":"813859"},{"id":"T112","span":{"begin":0,"end":6},"obj":"D011817"},{"id":"T114","span":{"begin":0,"end":6},"obj":"9986"},{"id":"T115","span":{"begin":68,"end":73},"obj":"CHEBI:30216"},{"id":"T116","span":{"begin":74,"end":87},"obj":"UBERON:0001135"},{"id":"T117","span":{"begin":74,"end":87},"obj":"UBERON:0000953"},{"id":"T120","span":{"begin":89,"end":94},"obj":"CHEBI:30216"},{"id":"T121","span":{"begin":99,"end":104},"obj":"P53491"},{"id":"T122","span":{"begin":99,"end":104},"obj":"P45520"},{"id":"T123","span":{"begin":99,"end":104},"obj":"O00937"},{"id":"T124","span":{"begin":99,"end":104},"obj":"Q05214"},{"id":"T125","span":{"begin":99,"end":104},"obj":"P13363"},{"id":"T126","span":{"begin":99,"end":104},"obj":"Q39596"},{"id":"T127","span":{"begin":99,"end":104},"obj":"P30161"},{"id":"T128","span":{"begin":99,"end":104},"obj":"P53476"},{"id":"T129","span":{"begin":99,"end":104},"obj":"P51775"},{"id":"T130","span":{"begin":99,"end":104},"obj":"P60011"},{"id":"T131","span":{"begin":99,"end":104},"obj":"Q9P4D1"},{"id":"T132","span":{"begin":99,"end":104},"obj":"P50138"},{"id":"T133","span":{"begin":99,"end":104},"obj":"O65314"},{"id":"T134","span":{"begin":99,"end":104},"obj":"P26183"},{"id":"T135","span":{"begin":99,"end":104},"obj":"Q75D00"},{"id":"T136","span":{"begin":99,"end":104},"obj":"P53502"},{"id":"T137","span":{"begin":99,"end":104},"obj":"P11426"},{"id":"T138","span":{"begin":99,"end":104},"obj":"P80709"},{"id":"T139","span":{"begin":99,"end":104},"obj":"Q8X119"},{"id":"T140","span":{"begin":99,"end":104},"obj":"Q6TCF2"},{"id":"T141","span":{"begin":99,"end":104},"obj":"Q99023"},{"id":"T142","span":{"begin":99,"end":104},"obj":"A5DQP9"},{"id":"T143","span":{"begin":99,"end":104},"obj":"P60009"},{"id":"T144","span":{"begin":99,"end":104},"obj":"O65315"},{"id":"T145","span":{"begin":99,"end":104},"obj":"P26182"},{"id":"T146","span":{"begin":99,"end":104},"obj":"Q9UVF3"},{"id":"T147","span":{"begin":99,"end":104},"obj":"Q2U7A3"},{"id":"T148","span":{"begin":99,"end":104},"obj":"Q92192"},{"id":"T149","span":{"begin":99,"end":104},"obj":"Q9UVZ8"},{"id":"T150","span":{"begin":99,"end":104},"obj":"P53689"},{"id":"T151","span":{"begin":99,"end":104},"obj":"P68555"},{"id":"T152","span":{"begin":99,"end":104},"obj":"P53455"},{"id":"T153","span":{"begin":99,"end":104},"obj":"O13419"},{"id":"T154","span":{"begin":99,"end":104},"obj":"P60010"},{"id":"T155","span":{"begin":99,"end":104},"obj":"Q39758"},{"id":"T156","span":{"begin":99,"end":104},"obj":"P81085"},{"id":"T157","span":{"begin":99,"end":104},"obj":"P48465"},{"id":"T158","span":{"begin":99,"end":104},"obj":"P78711"},{"id":"T159","span":{"begin":99,"end":104},"obj":"P17128"},{"id":"T160","span":{"begin":99,"end":104},"obj":"P91754"},{"id":"T161","span":{"begin":99,"end":104},"obj":"Q24733"},{"id":"T162","span":{"begin":99,"end":104},"obj":"P14235"},{"id":"T163","span":{"begin":99,"end":104},"obj":"Q92193"},{"id":"T164","span":{"begin":99,"end":104},"obj":"Q11212"},{"id":"T165","span":{"begin":99,"end":104},"obj":"P45521"},{"id":"T166","span":{"begin":99,"end":104},"obj":"P53477"},{"id":"T167","span":{"begin":99,"end":104},"obj":"P53499"},{"id":"T168","span":{"begin":99,"end":104},"obj":"P24902"},{"id":"T169","span":{"begin":99,"end":104},"obj":"Q8SWN8"},{"id":"T170","span":{"begin":99,"end":104},"obj":"O17320"},{"id":"T171","span":{"begin":99,"end":104},"obj":"P10365"},{"id":"T173","span":{"begin":99,"end":104},"obj":"O16808"},{"id":"T174","span":{"begin":99,"end":104},"obj":"P53498"},{"id":"T175","span":{"begin":99,"end":104},"obj":"Q9UVX4"},{"id":"T176","span":{"begin":99,"end":104},"obj":"O81221"},{"id":"T177","span":{"begin":99,"end":104},"obj":"P20904"},{"id":"T178","span":{"begin":99,"end":104},"obj":"O65316"},{"id":"T179","span":{"begin":99,"end":104},"obj":"O74258"},{"id":"T180","span":{"begin":99,"end":104},"obj":"P53500"},{"id":"T181","span":{"begin":99,"end":104},"obj":"P10989"},{"id":"T182","span":{"begin":99,"end":104},"obj":"P90689"},{"id":"T172","span":{"begin":99,"end":104},"obj":"D000199"},{"id":"T205","span":{"begin":106,"end":109},"obj":"CVCL_2501"},{"id":"T190","span":{"begin":106,"end":109},"obj":"GO:0047152"},{"id":"T199","span":{"begin":106,"end":109},"obj":"GO:0043791"},{"id":"T208","span":{"begin":106,"end":109},"obj":"GO:0043834"},{"id":"T224","span":{"begin":106,"end":113},"obj":"Q9XT90"},{"id":"T225","span":{"begin":106,"end":113},"obj":"P50281"},{"id":"T226","span":{"begin":106,"end":113},"obj":"Q9GLE4"},{"id":"T227","span":{"begin":106,"end":113},"obj":"PR:000010474"},{"id":"T228","span":{"begin":106,"end":113},"obj":"Q10739"},{"id":"T229","span":{"begin":106,"end":113},"obj":"Q95220"},{"id":"T231","span":{"begin":106,"end":113},"obj":"P53690"},{"id":"T230","span":{"begin":106,"end":113},"obj":"D053511"},{"id":"T234","span":{"begin":110,"end":113},"obj":"GO:0004235"},{"id":"T237","span":{"begin":115,"end":118},"obj":"CHEBI:340824"},{"id":"T240","span":{"begin":115,"end":118},"obj":"CHEBI:59761"},{"id":"T241","span":{"begin":115,"end":120},"obj":"PR:P08253"},{"id":"T242","span":{"begin":115,"end":120},"obj":"Q9GLE5"},{"id":"T243","span":{"begin":115,"end":120},"obj":"P08253"},{"id":"T244","span":{"begin":115,"end":120},"obj":"Q90611"},{"id":"T246","span":{"begin":115,"end":120},"obj":"PR:Q8MPP3"},{"id":"T247","span":{"begin":115,"end":120},"obj":"P33436"},{"id":"T248","span":{"begin":115,"end":120},"obj":"PR:Q90611"},{"id":"T249","span":{"begin":115,"end":120},"obj":"PR:P33436"},{"id":"T250","span":{"begin":115,"end":120},"obj":"PR:000010479"},{"id":"T251","span":{"begin":115,"end":120},"obj":"PR:P33434"},{"id":"T252","span":{"begin":115,"end":120},"obj":"P33434"},{"id":"T253","span":{"begin":115,"end":120},"obj":"P50757"},{"id":"T245","span":{"begin":115,"end":120},"obj":"GO:0004228"},{"id":"T254","span":{"begin":119,"end":125},"obj":"PR:O04529"},{"id":"T255","span":{"begin":122,"end":125},"obj":"CHEBI:340824"},{"id":"T258","span":{"begin":122,"end":125},"obj":"CHEBI:59761"},{"id":"T259","span":{"begin":122,"end":127},"obj":"PR:P50282"},{"id":"T260","span":{"begin":122,"end":127},"obj":"PR:P14780"},{"id":"T261","span":{"begin":122,"end":127},"obj":"P14780"},{"id":"T262","span":{"begin":122,"end":127},"obj":"PR:P41245"},{"id":"T263","span":{"begin":122,"end":127},"obj":"P41246"},{"id":"T264","span":{"begin":122,"end":127},"obj":"P41245"},{"id":"T265","span":{"begin":122,"end":127},"obj":"PR:P52176"},{"id":"T266","span":{"begin":122,"end":127},"obj":"P50282"},{"id":"T268","span":{"begin":122,"end":127},"obj":"PR:000010491"},{"id":"T269","span":{"begin":122,"end":127},"obj":"P52176"},{"id":"T270","span":{"begin":122,"end":127},"obj":"O18733"},{"id":"T267","span":{"begin":122,"end":127},"obj":"GO:0004229"},{"id":"T271","span":{"begin":133,"end":139},"obj":"UBERON:0000479"},{"id":"T272","span":{"begin":140,"end":149},"obj":"CHEBI:35222"},{"id":"T273","span":{"begin":153,"end":159},"obj":"UBERON:0027368"},{"id":"T278","span":{"begin":179,"end":186},"obj":"PR:P16035"},{"id":"T279","span":{"begin":179,"end":186},"obj":"PR:000016353"},{"id":"T280","span":{"begin":179,"end":186},"obj":"O42146"},{"id":"T281","span":{"begin":179,"end":186},"obj":"Q9TRZ7"},{"id":"T282","span":{"begin":179,"end":186},"obj":"Q9TTY1"},{"id":"T283","span":{"begin":179,"end":186},"obj":"Q60453"},{"id":"T284","span":{"begin":179,"end":186},"obj":"P16035"},{"id":"T286","span":{"begin":179,"end":186},"obj":"P25785"},{"id":"T287","span":{"begin":179,"end":186},"obj":"P16368"},{"id":"T288","span":{"begin":179,"end":186},"obj":"O77717"},{"id":"T289","span":{"begin":179,"end":186},"obj":"PR:P25785"},{"id":"T290","span":{"begin":179,"end":186},"obj":"P30121"},{"id":"T291","span":{"begin":179,"end":186},"obj":"Q9WUC6"},{"id":"T292","span":{"begin":179,"end":186},"obj":"PR:P30121"},{"id":"T293","span":{"begin":179,"end":186},"obj":"PR:O42146"},{"id":"T285","span":{"begin":179,"end":186},"obj":"D019716"},{"id":"T294","span":{"begin":255,"end":259},"obj":"C083283"},{"id":"T295","span":{"begin":255,"end":259},"obj":"C083283"},{"id":"T296","span":{"begin":281,"end":284},"obj":"PR:Q6RIA2"},{"id":"T297","span":{"begin":281,"end":284},"obj":"PR:Q15004"},{"id":"T300","span":{"begin":281,"end":284},"obj":"PR:000012225"},{"id":"T302","span":{"begin":281,"end":284},"obj":"PR:Q9CQX4"},{"id":"T301","span":{"begin":281,"end":284},"obj":"CVCL_XY57"},{"id":"T303","span":{"begin":281,"end":295},"obj":"CHEBI:134182"},{"id":"T305","span":{"begin":296,"end":304},"obj":"C066686"},{"id":"T306","span":{"begin":296,"end":304},"obj":"C066686"},{"id":"T307","span":{"begin":312,"end":317},"obj":"P09958"},{"id":"T308","span":{"begin":312,"end":317},"obj":"P23188"},{"id":"T309","span":{"begin":312,"end":317},"obj":"PR:000003137"},{"id":"T311","span":{"begin":312,"end":317},"obj":"PR:P09958"},{"id":"T312","span":{"begin":312,"end":317},"obj":"Q28193"},{"id":"T313","span":{"begin":312,"end":317},"obj":"PR:P23188"},{"id":"T314","span":{"begin":312,"end":317},"obj":"P23377"},{"id":"T315","span":{"begin":312,"end":317},"obj":"PR:P23377"},{"id":"T310","span":{"begin":312,"end":317},"obj":"D045683"},{"id":"T316","span":{"begin":318,"end":327},"obj":"CHEBI:35222"},{"id":"T317","span":{"begin":328,"end":332},"obj":"PR:Q04047"},{"id":"T318","span":{"begin":333,"end":338},"obj":"CHEBI:32693"},{"id":"T319","span":{"begin":333,"end":338},"obj":"CHEBI:32691"},{"id":"T320","span":{"begin":333,"end":338},"obj":"CHEBI:29953"},{"id":"T322","span":{"begin":335,"end":338},"obj":"PR:000013890"},{"id":"T324","span":{"begin":335,"end":338},"obj":"PR:P42684"},{"id":"T325","span":{"begin":335,"end":338},"obj":"PR:Q9P2R6"},{"id":"T327","span":{"begin":335,"end":338},"obj":"PR:Q4JIM5"},{"id":"T331","span":{"begin":335,"end":338},"obj":"PR:000003583"},{"id":"T321","span":{"begin":335,"end":338},"obj":"SO:0001451"},{"id":"T332","span":{"begin":335,"end":343},"obj":"C069339"},{"id":"T333","span":{"begin":335,"end":343},"obj":"C069339"},{"id":"T341","span":{"begin":346,"end":361},"obj":"GO:0010467"},{"id":"T342","span":{"begin":373,"end":378},"obj":"P09958"},{"id":"T343","span":{"begin":373,"end":378},"obj":"P23188"},{"id":"T344","span":{"begin":373,"end":378},"obj":"PR:000003137"},{"id":"T346","span":{"begin":373,"end":378},"obj":"PR:P09958"},{"id":"T347","span":{"begin":373,"end":378},"obj":"Q28193"},{"id":"T348","span":{"begin":373,"end":378},"obj":"PR:P23188"},{"id":"T349","span":{"begin":373,"end":378},"obj":"P23377"},{"id":"T350","span":{"begin":373,"end":378},"obj":"PR:P23377"},{"id":"T345","span":{"begin":373,"end":378},"obj":"D045683"},{"id":"T351","span":{"begin":380,"end":389},"obj":"D014568"},{"id":"T352","span":{"begin":380,"end":411},"obj":"GO:0008243"},{"id":"T371","span":{"begin":390,"end":411},"obj":"P17811"},{"id":"T372","span":{"begin":390,"end":411},"obj":"D010960"},{"id":"T373","span":{"begin":413,"end":416},"obj":"CHEBI:340824"},{"id":"T376","span":{"begin":413,"end":416},"obj":"CHEBI:59761"},{"id":"T377","span":{"begin":413,"end":418},"obj":"PR:P08253"},{"id":"T378","span":{"begin":413,"end":418},"obj":"Q9GLE5"},{"id":"T379","span":{"begin":413,"end":418},"obj":"P08253"},{"id":"T380","span":{"begin":413,"end":418},"obj":"Q90611"},{"id":"T382","span":{"begin":413,"end":418},"obj":"PR:Q8MPP3"},{"id":"T383","span":{"begin":413,"end":418},"obj":"P33436"},{"id":"T384","span":{"begin":413,"end":418},"obj":"PR:Q90611"},{"id":"T385","span":{"begin":413,"end":418},"obj":"PR:P33436"},{"id":"T386","span":{"begin":413,"end":418},"obj":"PR:000010479"},{"id":"T387","span":{"begin":413,"end":418},"obj":"PR:P33434"},{"id":"T388","span":{"begin":413,"end":418},"obj":"P33434"},{"id":"T389","span":{"begin":413,"end":418},"obj":"P50757"},{"id":"T381","span":{"begin":413,"end":418},"obj":"GO:0004228"},{"id":"T390","span":{"begin":417,"end":423},"obj":"PR:O04529"},{"id":"T391","span":{"begin":420,"end":423},"obj":"CHEBI:340824"},{"id":"T394","span":{"begin":420,"end":423},"obj":"CHEBI:59761"},{"id":"T395","span":{"begin":420,"end":425},"obj":"PR:P50282"},{"id":"T396","span":{"begin":420,"end":425},"obj":"PR:P14780"},{"id":"T397","span":{"begin":420,"end":425},"obj":"P14780"},{"id":"T398","span":{"begin":420,"end":425},"obj":"PR:P41245"},{"id":"T399","span":{"begin":420,"end":425},"obj":"P41246"},{"id":"T400","span":{"begin":420,"end":425},"obj":"P41245"},{"id":"T401","span":{"begin":420,"end":425},"obj":"PR:P52176"},{"id":"T402","span":{"begin":420,"end":425},"obj":"P50282"},{"id":"T404","span":{"begin":420,"end":425},"obj":"PR:000010491"},{"id":"T405","span":{"begin":420,"end":425},"obj":"P52176"},{"id":"T406","span":{"begin":420,"end":425},"obj":"O18733"},{"id":"T403","span":{"begin":420,"end":425},"obj":"GO:0004229"},{"id":"T429","span":{"begin":427,"end":430},"obj":"CVCL_2501"},{"id":"T414","span":{"begin":427,"end":430},"obj":"GO:0047152"},{"id":"T423","span":{"begin":427,"end":430},"obj":"GO:0043791"},{"id":"T432","span":{"begin":427,"end":430},"obj":"GO:0043834"},{"id":"T448","span":{"begin":427,"end":434},"obj":"Q9XT90"},{"id":"T449","span":{"begin":427,"end":434},"obj":"P50281"},{"id":"T450","span":{"begin":427,"end":434},"obj":"Q9GLE4"},{"id":"T451","span":{"begin":427,"end":434},"obj":"PR:000010474"},{"id":"T452","span":{"begin":427,"end":434},"obj":"Q10739"},{"id":"T453","span":{"begin":427,"end":434},"obj":"Q95220"},{"id":"T455","span":{"begin":427,"end":434},"obj":"P53690"},{"id":"T454","span":{"begin":427,"end":434},"obj":"D053511"},{"id":"T458","span":{"begin":431,"end":434},"obj":"GO:0004235"},{"id":"T465","span":{"begin":440,"end":446},"obj":"PR:P16035"},{"id":"T466","span":{"begin":440,"end":446},"obj":"PR:000016353"},{"id":"T467","span":{"begin":440,"end":446},"obj":"O42146"},{"id":"T468","span":{"begin":440,"end":446},"obj":"Q9TRZ7"},{"id":"T469","span":{"begin":440,"end":446},"obj":"Q9TTY1"},{"id":"T470","span":{"begin":440,"end":446},"obj":"Q60453"},{"id":"T471","span":{"begin":440,"end":446},"obj":"P16035"},{"id":"T473","span":{"begin":440,"end":446},"obj":"P25785"},{"id":"T474","span":{"begin":440,"end":446},"obj":"P16368"},{"id":"T475","span":{"begin":440,"end":446},"obj":"O77717"},{"id":"T476","span":{"begin":440,"end":446},"obj":"PR:P25785"},{"id":"T477","span":{"begin":440,"end":446},"obj":"P30121"},{"id":"T478","span":{"begin":440,"end":446},"obj":"Q9WUC6"},{"id":"T479","span":{"begin":440,"end":446},"obj":"PR:P30121"},{"id":"T480","span":{"begin":440,"end":446},"obj":"PR:O42146"},{"id":"T472","span":{"begin":440,"end":446},"obj":"D019716"},{"id":"T481","span":{"begin":476,"end":479},"obj":"Q41249"},{"id":"T482","span":{"begin":476,"end":479},"obj":"O66148"},{"id":"T483","span":{"begin":476,"end":479},"obj":"Q59987"},{"id":"T484","span":{"begin":476,"end":479},"obj":"Q01289"},{"id":"T485","span":{"begin":476,"end":479},"obj":"O80333"},{"id":"T486","span":{"begin":476,"end":479},"obj":"P15904"},{"id":"T487","span":{"begin":476,"end":479},"obj":"Q9SDT1"},{"id":"T488","span":{"begin":476,"end":479},"obj":"Q39617"},{"id":"T489","span":{"begin":481,"end":488},"obj":"CHEBI:16541"},{"id":"T490","span":{"begin":530,"end":533},"obj":"CHEBI:340824"},{"id":"T493","span":{"begin":530,"end":533},"obj":"CHEBI:59761"},{"id":"T494","span":{"begin":530,"end":535},"obj":"PR:P08253"},{"id":"T495","span":{"begin":530,"end":535},"obj":"Q9GLE5"},{"id":"T496","span":{"begin":530,"end":535},"obj":"P08253"},{"id":"T497","span":{"begin":530,"end":535},"obj":"Q90611"},{"id":"T499","span":{"begin":530,"end":535},"obj":"PR:Q8MPP3"},{"id":"T500","span":{"begin":530,"end":535},"obj":"P33436"},{"id":"T501","span":{"begin":530,"end":535},"obj":"PR:Q90611"},{"id":"T502","span":{"begin":530,"end":535},"obj":"PR:P33436"},{"id":"T503","span":{"begin":530,"end":535},"obj":"PR:000010479"},{"id":"T504","span":{"begin":530,"end":535},"obj":"PR:P33434"},{"id":"T505","span":{"begin":530,"end":535},"obj":"P33434"},{"id":"T506","span":{"begin":530,"end":535},"obj":"P50757"},{"id":"T498","span":{"begin":530,"end":535},"obj":"GO:0004228"},{"id":"T507","span":{"begin":570,"end":577},"obj":"4716"},{"id":"T508","span":{"begin":570,"end":577},"obj":"D005780"},{"id":"T509","span":{"begin":570,"end":577},"obj":"CHEBI:5291"},{"id":"T532","span":{"begin":597,"end":600},"obj":"CVCL_2501"},{"id":"T517","span":{"begin":597,"end":600},"obj":"GO:0047152"},{"id":"T526","span":{"begin":597,"end":600},"obj":"GO:0043791"},{"id":"T535","span":{"begin":597,"end":600},"obj":"GO:0043834"},{"id":"T551","span":{"begin":597,"end":604},"obj":"Q9XT90"},{"id":"T552","span":{"begin":597,"end":604},"obj":"P50281"},{"id":"T553","span":{"begin":597,"end":604},"obj":"Q9GLE4"},{"id":"T554","span":{"begin":597,"end":604},"obj":"PR:000010474"},{"id":"T555","span":{"begin":597,"end":604},"obj":"Q10739"},{"id":"T556","span":{"begin":597,"end":604},"obj":"Q95220"},{"id":"T558","span":{"begin":597,"end":604},"obj":"P53690"},{"id":"T557","span":{"begin":597,"end":604},"obj":"D053511"},{"id":"T561","span":{"begin":601,"end":604},"obj":"GO:0004235"}],"text":"Rabbit corneal myofibroblasts were identified by immunodetection of alpha-smooth muscle (alpha-SM)-actin. MT1-MMP, MMP-2, MMP-9, and tissue inhibitor of matrix metalloproteinase (TIMP)-2 were detected by immunofluorescence. Cells were treated with 100 nM cPAF, with or without the PAF antagonist BN 50730 or the furin inhibitor nona-D-arg-NH(2). Gene-expression levels for furin, urokinase plasminogen activator, MMP-2, MMP-9, MT1-MMP, and TIMP-2 were determined by real-time PCR. Protein expression was assessed by Western blot. MMP-2 and -9 activity was determined by gelatin zymography. Active MT1-MMP levels were measured by ELISA."}

{"project":"UseCases_ArguminSci_Discourse","denotations":[{"id":"T4","span":{"begin":0,"end":105},"obj":"DRI_Background"},{"id":"T5","span":{"begin":106,"end":223},"obj":"DRI_Background"},{"id":"T6","span":{"begin":224,"end":345},"obj":"DRI_Approach"},{"id":"T7","span":{"begin":346,"end":480},"obj":"DRI_Background"},{"id":"T8","span":{"begin":481,"end":529},"obj":"DRI_Background"},{"id":"T9","span":{"begin":530,"end":589},"obj":"DRI_Unspecified"},{"id":"T10","span":{"begin":590,"end":635},"obj":"DRI_Background"}],"text":"Rabbit corneal myofibroblasts were identified by immunodetection of alpha-smooth muscle (alpha-SM)-actin. MT1-MMP, MMP-2, MMP-9, and tissue inhibitor of matrix metalloproteinase (TIMP)-2 were detected by immunofluorescence. Cells were treated with 100 nM cPAF, with or without the PAF antagonist BN 50730 or the furin inhibitor nona-D-arg-NH(2). Gene-expression levels for furin, urokinase plasminogen activator, MMP-2, MMP-9, MT1-MMP, and TIMP-2 were determined by real-time PCR. Protein expression was assessed by Western blot. MMP-2 and -9 activity was determined by gelatin zymography. Active MT1-MMP levels were measured by ELISA."}