| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-52 |
Sentence |
denotes |
Staphylococcus aureus in inflammatory bowel disease. |
| T2 |
53-64 |
Sentence |
denotes |
BACKGROUND: |
| T3 |
65-232 |
Sentence |
denotes |
The potential role of superantigens in inflammatory bowel disease (IBD), particularly Crohn disease, has been broached in studies of the functions of T cell receptors. |
| T4 |
233-308 |
Sentence |
denotes |
Staphylococcal cells have been found in intestinal lymph follicles of IBDs. |
| T5 |
309-529 |
Sentence |
denotes |
To clarify a role of staphylococcal superantigens in IBD, we attempted to determine whether Staphylococcus aureus could be detected in intestinal mucosa, including surgical specimens and lymph follicles of initial cases. |
| T6 |
530-538 |
Sentence |
denotes |
METHODS: |
| T7 |
539-719 |
Sentence |
denotes |
One-hundred-and-six colonic and ileal specimens were obtained from 38 Crohn disease, 25 ulcerative colitis and 36 non-IBD patients through therapeutic surgery or endoscopic biopsy. |
| T8 |
720-817 |
Sentence |
denotes |
In Crohn disease, 23 surgical specimens and 11 biopsy specimens from initial cases were included. |
| T9 |
818-931 |
Sentence |
denotes |
DNA was extracted with phenol-chloroform after homogenization and proteinase K treatment in 73 mucosal specimens. |
| T10 |
932-1051 |
Sentence |
denotes |
Using an inverted microscope, lymph follicle tissue was microdissected from the remaining 33, mostly biopsy, specimens. |
| T11 |
1052-1093 |
Sentence |
denotes |
DNA was then extracted by freeze-thawing. |
| T12 |
1094-1150 |
Sentence |
denotes |
A coagulase gene characteristic of S. aureus was sought. |
| T13 |
1151-1262 |
Sentence |
denotes |
A nested polymerase chain reaction was performed utilizing primers that amplify a region of the coagulase gene. |
| T14 |
1263-1352 |
Sentence |
denotes |
Polymerase chain reaction products were analyzed with polyacrylamide gel electrophoresis. |
| T15 |
1353-1361 |
Sentence |
denotes |
RESULTS: |
| T16 |
1362-1513 |
Sentence |
denotes |
Only one surgically resected colonic specimen, from a 42-year-old male ulcerative colitis patient, registered positive staphylocoagulase amplification. |
| T17 |
1514-1526 |
Sentence |
denotes |
CONCLUSIONS: |
| T18 |
1527-1645 |
Sentence |
denotes |
Staphylococcal superantigens are not involved in either the early lesions or the established lesions of Crohn disease. |
| T19 |
1646-1723 |
Sentence |
denotes |
However, S. aureus infection occasionally may occur during the course of IBD. |
| T1 |
0-52 |
Sentence |
denotes |
Staphylococcus aureus in inflammatory bowel disease. |
| T2 |
53-64 |
Sentence |
denotes |
BACKGROUND: |
| T3 |
65-232 |
Sentence |
denotes |
The potential role of superantigens in inflammatory bowel disease (IBD), particularly Crohn disease, has been broached in studies of the functions of T cell receptors. |
| T4 |
233-308 |
Sentence |
denotes |
Staphylococcal cells have been found in intestinal lymph follicles of IBDs. |
| T5 |
309-529 |
Sentence |
denotes |
To clarify a role of staphylococcal superantigens in IBD, we attempted to determine whether Staphylococcus aureus could be detected in intestinal mucosa, including surgical specimens and lymph follicles of initial cases. |
| T6 |
530-538 |
Sentence |
denotes |
METHODS: |
| T7 |
539-719 |
Sentence |
denotes |
One-hundred-and-six colonic and ileal specimens were obtained from 38 Crohn disease, 25 ulcerative colitis and 36 non-IBD patients through therapeutic surgery or endoscopic biopsy. |
| T8 |
720-817 |
Sentence |
denotes |
In Crohn disease, 23 surgical specimens and 11 biopsy specimens from initial cases were included. |
| T9 |
818-931 |
Sentence |
denotes |
DNA was extracted with phenol-chloroform after homogenization and proteinase K treatment in 73 mucosal specimens. |
| T10 |
932-1051 |
Sentence |
denotes |
Using an inverted microscope, lymph follicle tissue was microdissected from the remaining 33, mostly biopsy, specimens. |
| T11 |
1052-1093 |
Sentence |
denotes |
DNA was then extracted by freeze-thawing. |
| T12 |
1094-1150 |
Sentence |
denotes |
A coagulase gene characteristic of S. aureus was sought. |
| T13 |
1151-1262 |
Sentence |
denotes |
A nested polymerase chain reaction was performed utilizing primers that amplify a region of the coagulase gene. |
| T14 |
1263-1352 |
Sentence |
denotes |
Polymerase chain reaction products were analyzed with polyacrylamide gel electrophoresis. |
| T15 |
1353-1361 |
Sentence |
denotes |
RESULTS: |
| T16 |
1362-1513 |
Sentence |
denotes |
Only one surgically resected colonic specimen, from a 42-year-old male ulcerative colitis patient, registered positive staphylocoagulase amplification. |
| T17 |
1514-1526 |
Sentence |
denotes |
CONCLUSIONS: |
| T18 |
1527-1645 |
Sentence |
denotes |
Staphylococcal superantigens are not involved in either the early lesions or the established lesions of Crohn disease. |
| T19 |
1646-1723 |
Sentence |
denotes |
However, S. aureus infection occasionally may occur during the course of IBD. |