PubMed:11424320 JSONTXT

{"project":"Inflammaging","denotations":[{"id":"T1","span":{"begin":0,"end":52},"obj":"Sentence"},{"id":"T2","span":{"begin":53,"end":64},"obj":"Sentence"},{"id":"T3","span":{"begin":65,"end":232},"obj":"Sentence"},{"id":"T4","span":{"begin":233,"end":308},"obj":"Sentence"},{"id":"T5","span":{"begin":309,"end":529},"obj":"Sentence"},{"id":"T6","span":{"begin":530,"end":538},"obj":"Sentence"},{"id":"T7","span":{"begin":539,"end":719},"obj":"Sentence"},{"id":"T8","span":{"begin":720,"end":817},"obj":"Sentence"},{"id":"T9","span":{"begin":818,"end":931},"obj":"Sentence"},{"id":"T10","span":{"begin":932,"end":1051},"obj":"Sentence"},{"id":"T11","span":{"begin":1052,"end":1093},"obj":"Sentence"},{"id":"T12","span":{"begin":1094,"end":1150},"obj":"Sentence"},{"id":"T13","span":{"begin":1151,"end":1262},"obj":"Sentence"},{"id":"T14","span":{"begin":1263,"end":1352},"obj":"Sentence"},{"id":"T15","span":{"begin":1353,"end":1361},"obj":"Sentence"},{"id":"T16","span":{"begin":1362,"end":1513},"obj":"Sentence"},{"id":"T17","span":{"begin":1514,"end":1526},"obj":"Sentence"},{"id":"T18","span":{"begin":1527,"end":1645},"obj":"Sentence"},{"id":"T19","span":{"begin":1646,"end":1723},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":52},"obj":"Sentence"},{"id":"T2","span":{"begin":53,"end":64},"obj":"Sentence"},{"id":"T3","span":{"begin":65,"end":232},"obj":"Sentence"},{"id":"T4","span":{"begin":233,"end":308},"obj":"Sentence"},{"id":"T5","span":{"begin":309,"end":529},"obj":"Sentence"},{"id":"T6","span":{"begin":530,"end":538},"obj":"Sentence"},{"id":"T7","span":{"begin":539,"end":719},"obj":"Sentence"},{"id":"T8","span":{"begin":720,"end":817},"obj":"Sentence"},{"id":"T9","span":{"begin":818,"end":931},"obj":"Sentence"},{"id":"T10","span":{"begin":932,"end":1051},"obj":"Sentence"},{"id":"T11","span":{"begin":1052,"end":1093},"obj":"Sentence"},{"id":"T12","span":{"begin":1094,"end":1150},"obj":"Sentence"},{"id":"T13","span":{"begin":1151,"end":1262},"obj":"Sentence"},{"id":"T14","span":{"begin":1263,"end":1352},"obj":"Sentence"},{"id":"T15","span":{"begin":1353,"end":1361},"obj":"Sentence"},{"id":"T16","span":{"begin":1362,"end":1513},"obj":"Sentence"},{"id":"T17","span":{"begin":1514,"end":1526},"obj":"Sentence"},{"id":"T18","span":{"begin":1527,"end":1645},"obj":"Sentence"},{"id":"T19","span":{"begin":1646,"end":1723},"obj":"Sentence"}],"text":"Staphylococcus aureus in inflammatory bowel disease.\nBACKGROUND: The potential role of superantigens in inflammatory bowel disease (IBD), particularly Crohn disease, has been broached in studies of the functions of T cell receptors. Staphylococcal cells have been found in intestinal lymph follicles of IBDs. To clarify a role of staphylococcal superantigens in IBD, we attempted to determine whether Staphylococcus aureus could be detected in intestinal mucosa, including surgical specimens and lymph follicles of initial cases.\nMETHODS: One-hundred-and-six colonic and ileal specimens were obtained from 38 Crohn disease, 25 ulcerative colitis and 36 non-IBD patients through therapeutic surgery or endoscopic biopsy. In Crohn disease, 23 surgical specimens and 11 biopsy specimens from initial cases were included. DNA was extracted with phenol-chloroform after homogenization and proteinase K treatment in 73 mucosal specimens. Using an inverted microscope, lymph follicle tissue was microdissected from the remaining 33, mostly biopsy, specimens. DNA was then extracted by freeze-thawing. A coagulase gene characteristic of S. aureus was sought. A nested polymerase chain reaction was performed utilizing primers that amplify a region of the coagulase gene. Polymerase chain reaction products were analyzed with polyacrylamide gel electrophoresis.\nRESULTS: Only one surgically resected colonic specimen, from a 42-year-old male ulcerative colitis patient, registered positive staphylocoagulase amplification.\nCONCLUSIONS: Staphylococcal superantigens are not involved in either the early lesions or the established lesions of Crohn disease. However, S. aureus infection occasionally may occur during the course of IBD."}

{"project":"disease_gene_microbe_small","denotations":[{"id":"T0","span":{"begin":25,"end":51},"obj":"DOID:0050589"},{"id":"T1","span":{"begin":105,"end":131},"obj":"DOID:0050589"},{"id":"T2","span":{"begin":152,"end":165},"obj":"DOID:8778"},{"id":"T3","span":{"begin":610,"end":623},"obj":"DOID:8778"},{"id":"T4","span":{"begin":628,"end":646},"obj":"DOID:8577"},{"id":"T5","span":{"begin":724,"end":737},"obj":"DOID:8778"},{"id":"T6","span":{"begin":1434,"end":1452},"obj":"DOID:8577"},{"id":"T7","span":{"begin":1632,"end":1645},"obj":"DOID:8778"},{"id":"T8","span":{"begin":0,"end":21},"obj":"taxonomy:1280"},{"id":"T9","span":{"begin":234,"end":248},"obj":"taxonomy:1279"},{"id":"T10","span":{"begin":331,"end":345},"obj":"taxonomy:1279"},{"id":"T11","span":{"begin":402,"end":423},"obj":"taxonomy:1280"},{"id":"T12","span":{"begin":1130,"end":1139},"obj":"taxonomy:1280"},{"id":"T13","span":{"begin":1528,"end":1542},"obj":"taxonomy:1279"},{"id":"T14","span":{"begin":1656,"end":1665},"obj":"taxonomy:1280"}],"namespaces":[{"prefix":"DOID","uri":"http://disease-ontology.org/term/DOID:"},{"prefix":"taxonomy","uri":"https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info\u0026id="}],"text":"Staphylococcus aureus in inflammatory bowel disease.\nBACKGROUND: The potential role of superantigens in inflammatory bowel disease (IBD), particularly Crohn disease, has been broached in studies of the functions of T cell receptors. Staphylococcal cells have been found in intestinal lymph follicles of IBDs. To clarify a role of staphylococcal superantigens in IBD, we attempted to determine whether Staphylococcus aureus could be detected in intestinal mucosa, including surgical specimens and lymph follicles of initial cases.\nMETHODS: One-hundred-and-six colonic and ileal specimens were obtained from 38 Crohn disease, 25 ulcerative colitis and 36 non-IBD patients through therapeutic surgery or endoscopic biopsy. In Crohn disease, 23 surgical specimens and 11 biopsy specimens from initial cases were included. DNA was extracted with phenol-chloroform after homogenization and proteinase K treatment in 73 mucosal specimens. Using an inverted microscope, lymph follicle tissue was microdissected from the remaining 33, mostly biopsy, specimens. DNA was then extracted by freeze-thawing. A coagulase gene characteristic of S. aureus was sought. A nested polymerase chain reaction was performed utilizing primers that amplify a region of the coagulase gene. Polymerase chain reaction products were analyzed with polyacrylamide gel electrophoresis.\nRESULTS: Only one surgically resected colonic specimen, from a 42-year-old male ulcerative colitis patient, registered positive staphylocoagulase amplification.\nCONCLUSIONS: Staphylococcal superantigens are not involved in either the early lesions or the established lesions of Crohn disease. However, S. aureus infection occasionally may occur during the course of IBD."}