PMC:7796052 / 13977-14753 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7796052","sourcedb":"PMC","sourceid":"7796052","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7796052","text":"3.2.1. Ligand Displacement Assays\nRaloxifene was tested for its ability to displace reference ligands on human recombinant bradykinin B1 or B2 receptors, respectively, expressed in CHO cells at 20 µM. For this purpose, first saturation isotherms were obtained with reference ligands ([3H] des-Arg10-BK (0.35 nM) in the case of B1, and [3H]-bradykinin (0.2 nM) for B2) incubated for 60 min at room temperature. Nonspecific binding was evaluated by adding a reference compound (desArg9[Leu8]-BK at 10 µM in the case of B1 and BK at 1 µM for B2). Antagonism of raloxifene was measured as a percent inhibition of specific binding of [3H]-bradykinin as control, obtained in the presence of raloxifene at 20 µM and using as reference compounds desArg10-KD for B1 and NPC-567 for B2.","divisions":[{"label":"title","span":{"begin":0,"end":33}}],"tracks":[{"project":"LitCovid-PubTator","denotations":[{"id":"356","span":{"begin":340,"end":350},"obj":"Gene"},{"id":"357","span":{"begin":634,"end":644},"obj":"Gene"},{"id":"358","span":{"begin":524,"end":526},"obj":"Gene"},{"id":"359","span":{"begin":490,"end":492},"obj":"Gene"},{"id":"360","span":{"begin":299,"end":301},"obj":"Gene"},{"id":"361","span":{"begin":105,"end":110},"obj":"Species"},{"id":"362","span":{"begin":34,"end":44},"obj":"Chemical"},{"id":"363","span":{"begin":285,"end":287},"obj":"Chemical"},{"id":"364","span":{"begin":336,"end":338},"obj":"Chemical"},{"id":"365","span":{"begin":484,"end":488},"obj":"Chemical"},{"id":"366","span":{"begin":558,"end":568},"obj":"Chemical"},{"id":"367","span":{"begin":630,"end":632},"obj":"Chemical"},{"id":"368","span":{"begin":685,"end":695},"obj":"Chemical"},{"id":"369","span":{"begin":181,"end":184},"obj":"CellLine"}],"attributes":[{"id":"A356","pred":"tao:has_database_id","subj":"356","obj":"Gene:3827"},{"id":"A357","pred":"tao:has_database_id","subj":"357","obj":"Gene:3827"},{"id":"A358","pred":"tao:has_database_id","subj":"358","obj":"Gene:3827"},{"id":"A359","pred":"tao:has_database_id","subj":"359","obj":"Gene:3827"},{"id":"A360","pred":"tao:has_database_id","subj":"360","obj":"Gene:3827"},{"id":"A361","pred":"tao:has_database_id","subj":"361","obj":"Tax:9606"},{"id":"A362","pred":"tao:has_database_id","subj":"362","obj":"MESH:D020849"},{"id":"A363","pred":"tao:has_database_id","subj":"363","obj":"MESH:D014316"},{"id":"A364","pred":"tao:has_database_id","subj":"364","obj":"MESH:D014316"},{"id":"A366","pred":"tao:has_database_id","subj":"366","obj":"MESH:D020849"},{"id":"A367","pred":"tao:has_database_id","subj":"367","obj":"MESH:D014316"},{"id":"A368","pred":"tao:has_database_id","subj":"368","obj":"MESH:D020849"},{"id":"A369","pred":"tao:has_database_id","subj":"369","obj":"CVCL:0213"},{"subj":"356","pred":"source","obj":"LitCovid-PubTator"},{"subj":"357","pred":"source","obj":"LitCovid-PubTator"},{"subj":"358","pred":"source","obj":"LitCovid-PubTator"},{"subj":"359","pred":"source","obj":"LitCovid-PubTator"},{"subj":"360","pred":"source","obj":"LitCovid-PubTator"},{"subj":"361","pred":"source","obj":"LitCovid-PubTator"},{"subj":"362","pred":"source","obj":"LitCovid-PubTator"},{"subj":"363","pred":"source","obj":"LitCovid-PubTator"},{"subj":"364","pred":"source","obj":"LitCovid-PubTator"},{"subj":"365","pred":"source","obj":"LitCovid-PubTator"},{"subj":"366","pred":"source","obj":"LitCovid-PubTator"},{"subj":"367","pred":"source","obj":"LitCovid-PubTator"},{"subj":"368","pred":"source","obj":"LitCovid-PubTator"},{"subj":"369","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T93","span":{"begin":0,"end":6},"obj":"Sentence"},{"id":"T94","span":{"begin":7,"end":33},"obj":"Sentence"},{"id":"T95","span":{"begin":34,"end":200},"obj":"Sentence"},{"id":"T96","span":{"begin":201,"end":409},"obj":"Sentence"},{"id":"T97","span":{"begin":410,"end":543},"obj":"Sentence"},{"id":"T98","span":{"begin":544,"end":776},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T93","pred":"source","obj":"LitCovid-sentences"},{"subj":"T94","pred":"source","obj":"LitCovid-sentences"},{"subj":"T95","pred":"source","obj":"LitCovid-sentences"},{"subj":"T96","pred":"source","obj":"LitCovid-sentences"},{"subj":"T97","pred":"source","obj":"LitCovid-sentences"},{"subj":"T98","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PubTator","color":"#e2ec93","default":true},{"id":"LitCovid-sentences","color":"#dc93ec"}]}]}}