PMC:7796052 / 13977-14753 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
356	340-350	Gene	denotes	bradykinin	Gene:3827
357	634-644	Gene	denotes	bradykinin	Gene:3827
358	524-526	Gene	denotes	BK	Gene:3827
359	490-492	Gene	denotes	BK	Gene:3827
360	299-301	Gene	denotes	BK	Gene:3827
361	105-110	Species	denotes	human	Tax:9606
362	34-44	Chemical	denotes	Raloxifene	MESH:D020849
363	285-287	Chemical	denotes	3H	MESH:D014316
364	336-338	Chemical	denotes	3H	MESH:D014316
365	484-488	Chemical	denotes	Leu8
366	558-568	Chemical	denotes	raloxifene	MESH:D020849
367	630-632	Chemical	denotes	3H	MESH:D014316
368	685-695	Chemical	denotes	raloxifene	MESH:D020849
369	181-184	CellLine	denotes	CHO	CVCL:0213

Id	Subject	Object	Predicate	Lexical cue
T93	0-6	Sentence	denotes	3.2.1.
T94	7-33	Sentence	denotes	Ligand Displacement Assays
T95	34-200	Sentence	denotes	Raloxifene was tested for its ability to displace reference ligands on human recombinant bradykinin B1 or B2 receptors, respectively, expressed in CHO cells at 20 µM.
T96	201-409	Sentence	denotes	For this purpose, first saturation isotherms were obtained with reference ligands ([3H] des-Arg10-BK (0.35 nM) in the case of B1, and [3H]-bradykinin (0.2 nM) for B2) incubated for 60 min at room temperature.
T97	410-543	Sentence	denotes	Nonspecific binding was evaluated by adding a reference compound (desArg9[Leu8]-BK at 10 µM in the case of B1 and BK at 1 µM for B2).
T98	544-776	Sentence	denotes	Antagonism of raloxifene was measured as a percent inhibition of specific binding of [3H]-bradykinin as control, obtained in the presence of raloxifene at 20 µM and using as reference compounds desArg10-KD for B1 and NPC-567 for B2.