PMC:7795856 / 5894-6282 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"164","span":{"begin":84,"end":87},"obj":"Gene"},{"id":"165","span":{"begin":329,"end":340},"obj":"Gene"},{"id":"167","span":{"begin":91,"end":98},"obj":"Species"}],"attributes":[{"id":"A164","pred":"tao:has_database_id","subj":"164","obj":"Gene:134864"},{"id":"A165","pred":"tao:has_database_id","subj":"165","obj":"Gene:7295"},{"id":"A167","pred":"tao:has_database_id","subj":"167","obj":"Tax:562"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":" degraded in the bacterial cytoplasm; thus, efficient one-step production of mature Tα1 in E. coli is not feasible. An alternative would be expression as part of a larger protein such as natural ProTα [26], as artificial concatamers [27], in fusion with an intein [28] or in fusion with a highly expressed bacterial protein like thioredoxin [29]. In each case, enzymatic or chemical cleav"}

{"project":"LitCovid-sentences","denotations":[{"id":"T39","span":{"begin":116,"end":346},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":" degraded in the bacterial cytoplasm; thus, efficient one-step production of mature Tα1 in E. coli is not feasible. An alternative would be expression as part of a larger protein such as natural ProTα [26], as artificial concatamers [27], in fusion with an intein [28] or in fusion with a highly expressed bacterial protein like thioredoxin [29]. In each case, enzymatic or chemical cleav"}